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K ( 86Rb ) - bioassay 를 이용한 감귤나무의 가리영양진단법 개발
유장걸(Zang Kual U),한해룡(Hae Ryong Han),문덕영(Duk Young Moon),김창명(Chang Myung Kim),임한철(Han Cheol Lim),문두경(Do Kyung Moon),송성준(Sung Jun Song) 한국응용생명화학회 1994 Applied Biological Chemistry (Appl Biol Chem) Vol.37 No.3
Similar to the ^(42)K uptake, ^(86)Rb uptake by the roots of Hordeum distichum grown in the hydroponic culture was negatively correlated with the concentration of K supplied previously, showing that ^(86)Rb can be used for the K-bioassay. ^(86)Rb having longer half life (18.86 day) than ^(42)K (12.36 hr) allowed the use of larger number of root samples. ^(86)Rb uptake of 3 years old Citrus unshiu Marc. grown in water culture decreased drastically with the increase of K concentration of the culture solution, thus demonstrating that the nutrition status of K for citrus trees can be diagnosed by K-bioassay using ^(86)Rb tracer. ^(86)Rb uptake by the excised roots of Hordeum distichum grown in the pot with different K fertilizations was well correlated with the exchangeable K in soil. The amount of exchangeable K in soil for the optimal plant growth can be determined by its relationship. ^(42)K and ^(86)Rb-uptake by the Hmdeum distichum roots were markedly inhibited by 5×10^(-3) M KCN in the bioassay solution, indicating that uptake is energy-dependent. There was no significant relationship between K content in citrus leaves and K concentration in the water-culture medium. It is concluded that K-bioassay is a potentially useful tool for determining of K requirement in citrus trees.