http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 문관우(Kwan Woo Moon) (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
-
Escherichia coli의 지방산 대사과정 및 배양조건 조절을 통한 FAEE 생산
문관우(Kwan Woo Moon),임승락(Seung Rak Lim),박혜민(Hye Min Park),윤현식(Hyun Shik Yun),김세경(Se Kyung Kim),백종윤(Jong Youn Baik) 한국생물공학회 2020 KSBB Journal Vol.35 No.3
Global warming is a major issue of modern climate change, and caused by the greenhouse effect mainly due to carbon dioxide emitted by burning fossil fuels. To reduce the emission of carbon dioxide, scientists have focused on replacing fossil fuels with renewable energy. Biofuel, one form of renewable energy derived from the carbon sources of natural biomass, can contribute to reduced carbon dioxide emission. In this study, Escherichia coli was engineered for the production of fatty acid ethyl ester (FAEE), biodiesel derived from fatty acids. For production of FAEE, pyruvate decarboxylase (pdc), alcohol dehydrogenase II (adhB), acyl-Coenzyme A : diacylglycerol acyltransferase (atfA), and fatty acid synthesis (FAS) pathway genes were selected and cloned on to various plasmids. We designed and introduced various combinations of expression constructs in E. coli to enhance FAEE production. As a result, a 3.88-fold increase in FAEE production was achieved by modifying the expression levels of the genes involved in the initiation step and the β-oxidation step. The production of FAEE at different growth phases as well as the effect of the culture conditions such as temperature and carbon sources on FAEE productivity were studied. As a future study, scale-up studies through fermenter cultivation would contribute to mass production of biodiesel.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
