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Trichoderma inhamatum KSJ1의 반연속배양 방식에 따른 섬유소분해효소의 생산성 비교
리홍선(Hongxian Li),김성준(Seong-Jun Kim) 한국생물공학회 2009 KSBB Journal Vol.24 No.1
본 연구에서는 음식물쓰레기를 당화시키기 위해 섬유소분해효소를 효율적으로 배양하고자 먼저 refill하는 멘델배지의 농도를 0.5%로, 새로운 배지의 주입시간을 12시간으로 결정하였다. Flask 레벨에서는 fill-and-draw 방법으로 12시간 단위로 연속배양한 결과, amylase 활성은 300시간까지 1.0 U/mL 내외로 유지되었으며, FPase 활성은 156시간까지 0.40 U/mL 이상으로 유지되었다. 이때의 효소생산성은 amylase 3.49 U/Lㆍhr, FPase 1.02 U/Lㆍhr 이었다. 10 L에서는 batch, fed-batch, fill-and-draw 방법으로 효소를 생산한 결과 batch에서 가장 높은 효소생산성을 나타내었으며, 그다음은 fed-batch 이었다. Batch에서의 효소생산성은 amylase 42.30 U/Lㆍhr, FPase 5.60 U/Lㆍhr, fed-batch 에서는 각각 23.03, 2.76 U/Lㆍhr 이었다. 그리하여 T. inhamatum KSJ1을 이용한 섬유소분해효소의 연속배양에 서 10 L 생물반응기에서 fed-batch 방법이 가장 효율적이었다 For continuous culture of cellulolytic enzymes production to saccharify food wastes, refill concentration of Mandel"s medium for continuous culture was 0.5%, and refill intervals were determined to 12 hours by analysis of COD and total nitrogen concentration after 4-days batch culture in flask level. As a result, amylase and FPase productivities were 3.5 and 1.0 U/Lㆍhr, respectively. In 10 L bioreactor, the batch culture mode was compared with fed-batch, fill-and-draw for continuous production of cellulolytic enzyme. Enzyme productivities were most high at batch culture and followed by fed-batch culture. Amylase and FPase activities were 42.3 and 5.6 U/Lㆍhr at batch culture, and 23.0, 2.8 U/Lㆍhr at fed-batch culture, respectively. As a result, in continuous cultivation of cellulolytic enzymes by T. inhamatum KSJ1, the mode of fed-batch was most effective in 10 L bioreactor.
에탄올 발효방법에 따른 음식물류 폐기물의 바이오에탄올 생산성 비교
강희정(Hee-Jeong Kang),리홍선(HongXian Li),김용진(Yong-Jin Kim),김성준(Seong-Jun Kim) 한국생물공학회 2010 KSBB Journal Vol.25 No.5
In order to improve bio-ethanol productivity by various cultivation methods in this paper, the culture modes using food wastes, such as batch culture, high-cell-density fermentation, SSF (simultaneous saccharification and fermentation) by fill & draw, continuous culture by fill & draw were performed and their productivities were compared. SSFs by fill & draw were performed by continuous decompression using 1 L evaporator system, and by 10 L bioreactor without decompression. In addition, the continuous cultures by fill & draw mode using SFW (saccharafied food wastes) medium were performed by changes of 40% culture broth with intervals of 12 h (0.03 h<SUP>-1</SUP>), 6 h (0.07 h<SUP>-1</SUP>), 3 h (0.13 h<SUP>-1</SUP>). Consequently, productivities of bio-ethanol were 2.52 g/L-h and 1.30 g/L-h in batch culture and high- cell-density fermentation, respectively. The productivities of SSF by fill & draw showed 2.24 g/L-h and 2.03 g/L-h in continuous decompression with 1 L evaporator and 10 L bioreactor without decompression, respectively. Also, the productivities in continuous culture by fill & draw modes showed 2.02 g/L-h, 4.07 g/L-h and 6.25 g/L-h by medium change with intervals of 12 h, 6 h, and 3 h, respectively. In conclusion, the highest ethanol productivity was obtained in the continuous culture mode by fill & draw with dilution rate of 0.13 h<SUP>-1</SUP>.