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피브로인 미세구 첨가에 의한 3T3 섬유아 세포의 이동 촉진
권세창(Se Chang Kwon),전현상(Hyun Sang Jeon),류다영(Da Yeong Ryu),김지영(Ji Young Kim),허원(Won Hur) 한국생물공학회 2018 KSBB Journal Vol.33 No.1
Microsphere-stimulated cell migration was observed at the periphery of a confluent 3T3 cell culture even in the absence of serum. Under non-contact inhibition conditions, 3T3 cells showed a highest migratory velocity of 1.7 μm/min in a DMEM media with 0.05 mg/mL microspheres. It is 147% faster than those in DMEM with 10% serum and 194% faster than those in DMEM only. Monolayer colonies were formed from 3T3 cell spheroids in a microsphere-containing culture medium but not in a DMEM without serum. Western blot analysis showed that microsphere supplementation reduced the expression levels of focal adhesion kinase. Fibroin microsphere seems to stimulate a migratory signal cue other than focal adhesion kinase. Unlike to other nanoparticles, fibroin microspheres stimulated 3T3 cell migration, showing a potential candidate for wound healing stimulation.