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율무, 보리, 미강 유기용매 추출물의 항산화능과 포도당 및 지방산 대사에 미치는 영향
박태식 ( Tae Sik Park ),이수연 ( Su Yeon Lee ),김현진 ( Hyun Jin Kim ),김경탁 ( Kyung Tack Kim ),김영준 ( Young Jun Kim ),정인혜 ( In Hye Jeong ),도완녀 ( Wan Nyo Do ),이혜정 ( Hye Jeong Lee ) 한국식품영양학회 2009 韓國食品營養學會誌 Vol.22 No.3
Adlay, barley and rice bran were extracted using various concentrations of methanol(10% and 80%) and chloroform: methanol(2:1) to examine the biological activities of these raw grains. Extraction with 80% methanol resulted in high Vitamin C Equivalent Antioxidant Capacity(VCEAC), in the order of barley>rice bran>adlay, as determined by DPPH and ABTS assays. In addition, the extracts of adlay and rice bran showed high cellular antioxidant activity in HepG2 cells possibly due to the presence of polyphenol glycosides in these grains. We examined the expression of glucose/fatty acid metabolizing genes in differentiated 3T3-L1 adipocyte cells. Glut1 was downregulated after treatment with rice bran and no changes in the expression of Glut4 was observed. In contrast, genes involved in fatty acid metabolism, CD36 and aP2, were upregulated. Since these physiological changes were matched with peroxisome proliferator activating receptor γ(PPAR γ) agonism, we suggest that the extracts from adlay, barley and rice bran may play preventive roles against aging and diabetes via antioxidant activity and increased uptake of fatty acids by adipocytes.