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誘導 된 敗血症 모델에서 Aprotinin 과 Insulin 이 iNOS m-RNA 에 미치는 영향
유길준,백승완,노춘국 대한마취과학회 1998 Korean Journal of Anesthesiology Vol.34 No.6
Background : Nitric oxide(NO) has been known to have antimicrobial activity, and the increased NO production in case of sepsis may play as a physiologic defense mechanism. However the increased formation of NO by the inducible nitric oxide synthase(iNOS) has been known to be implicated in pathophysiology of a variety of disease, including circulatory and septic shock. We measured the iNOS activities of mouse macrophage after application of various drugs with lipopolysaccharide(LPS) and γ-interferon(IFN). The purpose of this study is to evaluate the inhibiting properties of various drugs to iNOS in case of sepsis. Method : Thirty ICR(Institue for Cancer Research) mouse weighting 30∼40 gm were anesthetized with diethyl ether, and thiogycol broth was injected into peritoneal cavity. Two days later macrophages were collected from peritoneal cavity, and incubated for 24 hours in the CO2 incubator with LPS and IFN mixture and various concentration of dexamethasone, pentoxifylline, aspirin, aprotinin, regular insun(RI) and neutral protamine hagedorn insulin(NPH). NO concentration were calculating by measuring nitrite concentration which represent the magnitude of NO production. The activities of iNOS in macrophages were measured by analysing iNOS m-RNA expression by Northern blot analysis with autoradiography using polymerase chain reaction(PCR) method. Result : The basic NO concentration was 13.0 8.0 M. With LPS and IFN, NO concentration was increased to 104.4 31.9 M. The increase in NO production by LPS and IFN was attenuated by dexamethasone(10 6 M only), pentoxifylline(above 10 10 M), aprotinin, RI, and NPH in dose dependent manner. The addition of LPS and IFN in the culture media caused increase in the iNOS m-RNA production. The aprotinin, RI, and NPH attenuated the increase in iNOS m-RNA production by LPS and IFN. Coclusion : These results suggest that the aprotinin, RI, and NPH prevent the LPS and IFN induced increase of NO production by attenuating the iNOS activity. These properties of aprotinin and insulin may be applied to the treatment of septic shock to block the enhanced formation of NO production. (Korean J Anesthesiol 1998; 34: 1086∼1096)
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권재영,백승완,김해규,정규섭,김인세,노춘국 대한마취과학회 1991 Korean Journal of Anesthesiology Vol.24 No.5
This study examined the behavioral change and memory impairment following transient forebrain ischemia in 20 male rats: ten rats were subjected to sham operation. Inchemia was induced by a combination of bilateral common carotid artery occlusion and induced hypotension. During the recovery period, behavioral change was evaluated by open-field method. Histopathological examination was performed 7 days after ischemic insult. The results were as follows 1) Hippocampus CA 1 neuron was the most vulnerable to 10-minute forebrain ischemia. 2) There was increase in locomotor activity and rearing during early post-ischemic period. 3) Ischemia resulted in suppressed preening and grooming. From the above mentioned results, authors suggested that transient forebrain ischemia produced severe neuronal damage in CA l sector of hippocampus, therefore produced behavioral change and memory impairment.
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