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우유에서 분리한 호냉성세균의 Heat-Stable Protease 생산조건
박우열,박법규,류병호,김희숙,노명훈 慶星大學校 1990 論文集 Vol.11 No.2
Pseudomonas fluorescens BH-22, which produced a heat stable protease, was isolated from the city milk. The optimum medium for heat stable protease production from the microorganism was as follows ; glucose 3, casein 5, KH₂PO₄ 0.1, MgSO₄ . 7H₂O 0.1 and CaCO₃ 0.1g100ml. The optimum pH of medium for heat stable protease production was pH 8.0, temperature was 20˚C. Proteolytic culture of Pseudomonas fluorescens BH-22 lost the protease activity ranged from 15% to 18% on heating for 30 min at 55˚C and for 20 min at 60˚C. This study was identified to excrete the protease by psychrotropic bacteria in city milk, and also investigated to the optical conditions of protease production and heat stability of the enzyme. The morphological and physiological characteristics of the strain BH-22 were studied according to the methods of Bergey's mannual, Gradwohl's clinical laboratory method and API 20NE Kit. From these results, strain BH-22 was identified as Pseudomonas fluorescens.