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재조합 인간 상피세포성장인자(DWP401)의 흰쥐에서의 in vivo와 in vitro 대사
고여욱(Yeo Wook Koh),남권호(Kouen Ho Nam),정주영(Ju Young Jung),박승국(Seung Kook Park),유영효(Young Hyo Yu),김재환(Jae Hwan Kim),한건(Kun Han),박명환(Myung Hwan Park),심창구(Chang Koo Shim) 대한약학회 1997 약학회지 Vol.41 No.3
Metabolism of DWP401, recombinant juman epidermal growth factor, was examined in vivo and in vitro in rats. When 125I-labeled DWP401 was administered at a dose of 50 mcg/kg by i.v. injection. 125I-labeled DWP401 was rapidly degraded within 30 minytes above 93%. Thin layer chromatography analysis of urine collected for 24 hr after i.v. administration of 125I-labeled DWP401 showed ohly one spot on a X-ray film which was considered as diiodo-tyrosine. This result suggests tha 125I-labeled DWP401 was completely digested into free amino acids without any specific intermediate polypeptides. About 42.1% of the administered iodine was recovered in 24 hr. For in vitro degradation study, 125I-labeled DWP401 was added to plama and tissue homogenates of rats and incubated at 37oC. Almost 98% of the added radioactivity recovered from the protein fraction of the liver, kidey, small intestine, stomach and spleen decreased rapidly. For examplem the recovery rates of 125I-labeled DWP401 were 58.6, 63.2, 39.9, 52.9 and 66.8% after 4hrs of incubation in respective organ homogenates.