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제주지역의 akabane virus에 대한 역학조사 및 원인체 분리동정
강완철 ( Wan Cheul Kang ),김은주 ( Eun Joo Kim ),현관종 ( Kwan Jong Hyun ),전창익 ( Chang Ik Cheon ),김희석 ( Hye Seck Kim ),이두식 ( Du Sik Lee ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.1
In this experiment, we studied the sero-positive rate of akabane virus in cattle from Jeju-do and analyzed the seroepidemiological features. In an analysis of 1051 samples, the positive rate for neutralizing antibody in sera collected in nine regions on Jeju-do was 56.7%. The rate varied with the region. The positive rate was 69.6% in Aewol, 63.1% in Jeju city, 54.4% in Anduck, 51.0% in Hallim, 69.8% in Jocheun, 47.6% in Pyosun, 40% in Daejeong, 30.0% in Flankyung, 71.6% in Namwon, 24.5% in Sungsan, 83.3% in Seokypo and 44.5% in Gujwa, respectively. The rate also depended on the age of the cattle. The positive rate was 67.2% in calves 0- to 12-month old, 48.3% in cattle 13- to 24-month old, 65.4% in cattle 25- to 36-month old, arid 65.4% in cattle more than 37 months old. To isolate the virus from calves with malformations including arthrogryposis and hydranencephaly, cerebral homogenates were inoculated into Vero cells, which were determined for cytopathic effect (CPE). Vero cells with CPE were examined for Akabane virus using an electron microscope (EM) and indirect immunofluorescent antibody test (WA). Typical virus particles with a width of 90-l3Onm and specific immunofluorescence in the cytoplasm of infected cells were sought for identification.