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L-ascorbic acid와 α-tocophero1이 돼지정액 성상과 체외수정란의 배발달율에 미치는 영향
박희진,김은국,위갑인,강만종,김광현,김재홍,문승주 全南大學校 農業科學技術硏究所 2001 農業科學技術硏究 Vol.36 No.-
The objective of this studys were to investigate the effect of α-tocopherol and L-ascorbic acid supplement on the boar semen characteristics and in vitro development rate of porcine oocytes. Sperm viability rate was significantly higher in α-tocopherol supplement than in control(66.6%) after 72hr of storage(P<0.05). Sperm motility was increased in treatment(80%, 93.3%, 85%) than in control(73.3%) at 12h of storage(P<0.05). Following insemination with 60h of store, morular and blastocyst on in vitro development resulted in significantly higher than control(P<0.05). After 72h of semen storage L-ascorbic addition also increased the sperm viability rate(P<0.05). Treatment as sperm motility was significantly higher than control in 72h of store(60% vs 66.6%, p<0.05). Following insemination with 60h of store treated porcine oocytes on in vitro development was increased more than control(56.8%), 59.9%, 62.8% vs 45.1%, P<0.05). Morular and blastocyst on in vitro development resulted in significantly higher than control(27.1%, 30.0%, 33.1% vs 20.0%, P<0.05). The results from this experiment indicated that α-tocopherol and L-ascorbic acid supplementation, for viability and motility in porcine semen, increased delay storing time of semen and in vitro development rate following insemination.
항산화제가 돼지난포란의 체외성숙, 체외수정 및 체외배발달에 미치는 영향
김민식,김은국,위갑인,강만종,문승주,김재홍,조미영,박지현 全南大學校 農業科學技術硏究所 2001 農業科學技術硏究 Vol.36 No.-
This study was conducted to investigate effects of L-ascorbic acid, selenium, α-tocopherol, cysteamine and glutathione on maturation, development ability of porcine oocytes in vitro. When the immature oocytes were cultured of L-ascorbic acid, selenium, α-tocopherol, cysteamine and glutathione for 40-44hr, the nuclear maturation rates were 39.4, 80.9, 82.2, 65.6, 49.1 and 87.9%, respectively. The nuclear maturation rates of treatment groups were significantly(P<0.05) higher than those of non-treat groups. After maturation, the oocytes were inseminated in vitro in medium 199 with ejaculated spermatozoa for examination of cleavage rate and development ability of porcine oocytes. After insemination, the cleavage rates at 48hr of treat groups were higher than thecontrol group(P<0.05), and the cleavage rates at 120hr of treat groups were higher than control group(P<0.05).