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신령버섯균사체 액체배양물의 자가분해에 의한 항암성 isoflavone-conjugated glycoprotein 분리
김소영(So Young Kim),김영숙(Young Suk Kim),장정순(Joung Soon Jang),김보현(Boh Hyun Kim),라키브 압두르(Abdur Md. Rakib),김곤섭(Gon Sup Kim),김정옥(Jeong Ok Kim),하영래(Yeong Lae Ha) 한국생명과학회 2014 생명과학회지 Vol.24 No.12
신령버섯균사체(Agaricus blazei mycelia: ABM)를 대두박이 함유된 액체배지에 배양하고, 이것을 자가분해(53 ℃, pH 5.5, 120 rpm, 3 hr)하여 항암성이 강한 isoflavone-conjugated glycoprotein (Gluvone 이라 명명)을 분리하였다. Gluvone 은 지금까지 알려진 당단백질과는 달리 분자량이 작고(9,400 Da), isoflavone이 결합되어 있다는 점이 다르다, Gluvone 은 60% 탄수화물(glucose, fructose, ribose), 31% 단백질 및 2% isoflavone (daidzein, genistein)으로 구성되어 있었다. 이 Gluvone은 S-180 복수암세포, MCF-7 인체유선암세포에 대한 독성이 강하였고, S-180 세포로 유발한 mouse 복수암을 강하게 억제하였다. Most beta-glucans obtained from various fruit bodies of mushrooms and mushroom mycelial cultures have high-molecular weight glycoproteins, conjugated with beta-glucans. We report that isoflavone- conjugated glycolproteins (designated as gluvone) were isolated and exhibited stronger anticarcinogenic activities. Agaricus blazei mycelia (ABM) was cultured in a liquid medium containing soybean flakes for 14 days. The liquid culture was autolyzed by incubating at 53℃ (pH 5.5) for 3 h. A crude glycoprotein (CGP) fraction with a cytotoxic effect on a mouse ascite cancer cell line (S-180) and a human breast cancer cell line (MCF-7) was isolated from the autolyzed ABM cultures by 80% ethanol treatment. Gluvone was isolated from the CGP with Sephadex G-75 column chromatography. It exhibited a stronger anticancer effect than CGP against the S-180 cell-induced female ICR mouse ascites carcinogenesis. Gluvone with 9,400 daltons was identified as a glycoprotein conjugated with isoflavone. According to HPLC and GC analysis, in conjunction with ¹H-NMR spectral analysis, it contained 60% carbohydrates (glucose, fructose, and ribose), 31% protein, and 2% isoflavone (daidzein and genistein), which is a novel material. These results indicate that a strong anticarcinogenic gluvone was isolated from the autolyzed product of a submerged liquid culture of ABM, suggesting that autolysis could be a useful tool to produce antitumor agents.
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자연스러운 여성 합성음을 위한 한국어의 피치 변화 법칙
김중원,박대덕,김보현,권철홍,Kim, Chung-Won,Park, Dae-Duck,Kim, Boh-Hyun,Kwon, Cheol-Hong 한국음향학회 1996 韓國音響學會誌 Vol.15 No.6
본 논문은 자연스러운 여성 합성음을 위한 피치 변화 법칙을 세웠다. 피치 변화 법칙이 적용되는 기본 단위, 즉 억양구는 주로 어절(들)로 이것의 첫번째, 두번째, 마지막 음절의 피치값을 연결해 피치 변화 곡선을 형성하였는데, 첫번째, 두번째 음절의 피치값은 각 음절의 초성에 따라, 마지막 음절의 피치값은 기능어의 종류에 따라 결정되었다. 억양구 사이에는 '쉼(pause)이 있는 경계' 또는 '쉼이 없는 경계'가 오며, 쉼이 있는 경계에는 relaxation이 있다. 이러한 억양구의 피치 변화 곡선, 경계 현상들이 모여 한 문장의 피치 턴을 만들었다. In this paper we make a rule of pitch variation for a natural synthetic female voice. Intonation phrase, which is the basic unit the rule is applied to, mostly consists of a syllable or syllables. The pitch values of the first, second, and final syllables make up the pitch contour of the intonation phrase. Those of the first and second syllable are determined by the initial consonants of the respective syllables, and that of the final syllable by the type of the function word. There are two kinds of boundaries between intonation phrases. One is a boundary with pause, and the other is a boundary without pause. The pitch contour of the intonation phrase with the boundary phenomena determines the pitch pattern of a sentence.
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Anti-proliferative Effects of β-ionone on Human Lung Cancer A-549 Cells
Sun Min Lee(이선민),Young Sook Kim(김영숙),Wook Jin Jang(장욱진),Abdur Md. Rakib(압두르 라키브),Tae Woo Oh(오태우),Boh Hyun Kim(김보현),So Young Kim(김소영),Jeong Ok Kim(김정옥),Yeong Lae Ha(하영래) 한국생명과학회 2013 생명과학회지 Vol.23 No.11
β-Ionone의 인체 비소폐암세포 A-549 cells (human non-small lung cancer A-549 cell)에 대한 anti-proliferative effect에 관한 연구를 수행하였다. A-549 cell에 다양한 농도의 β-ionone (1, 5, 10, and 15 μM)을 2, 4, 6일 간 처리 하고, 배양 2일에 A-549 cell의 생육억제와 관련되는 biochemical marker를 측정하였다. β-Ionone은 A-549 cell 생육을 dose와 time 의존적으로 저해하였다. β-Ionone 배양 2일의 IC50은 5.0 μg/ml이었다. β-Ionone은 농도 의존적으로 apoptosis를 유도하였다. β-Ionone은 p53, p21 및 Bax protein 수준을 증가시켰으나, Bcl-2 protein 발현은 억제시켰다. β-ionone은 cytosol cytochrome c 함량을 증가시켰고, caspase-9과 caspase-3 효소 활성증가를 유도하였다. 또한, β-ionone은 cPLA₂와 COX-2 protein level을 감소시켰다. 이와 같은 결과는 β-ionone의 A-549 cell에 대한 생육억제효과는 Bax와 Bcl-2 gene 발현을 reciprocal regulation하여 유도한 apoptosis와 cPLA₂ 및 COX-2 protein 발현 억제에 기인함을 의미한다. The anti-proliferative activity of β-ionone was investigated on human non-small lung cancer A-549 cells (designated A-549 cells). A-549 cells were treated with various concentrations of β-ionone (1, 5, 10, and 15 μM) for two, four, and six days. Biochemical markers related to the growth inhibition of A-549 cells by β-ionone were measured at the second day of incubation. β-Ionone inhibited the growth of A-549 cells by dose-and time-dependent manners, resulting in an IC50 of 5.0 μg/ml at the second day of incubation. β-Ionone induced apoptosis by a dose-dependent manner. β-Ionone increased levels of p53, p21, and Bax proteins, but suppressed expression of the Bcl-2 protein. Similarly, β-ionone enhanced cytochrome c release from the mitochondria to the cytosol, and induced activation of caspase-9 and -3. Additionally, β-ion-one reduced cPLA₂ and COX-2 protein levels. These results suggest that the β-ionone inhibits the proliferation of A-549 cells through reciprocal regulation of Bax and Bcl-2 gene expression and suppression of cPLA₂ and COX-2 protein expressions.
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조을제,김보현,장석주 大韓成形外科學會 1994 Archives of Plastic Surgery Vol.21 No.2
Reconstruction of soft tissue defects with exposure of bone or tendons on the hands can be a difficult problem. Local subcutaneous tissue flaps with immediate skin graft were successfully used to reconstruct the soft tissue defect of hand in 4 cases of 3 patients. All flaps survive completley with no major problems. Among various technique the benefits of this flap are that it is and easy and rapid one-Stage procedure, it requires limited immobilization of the involved joint, it leaves an inconspicuous donor site scar and it preserving the shape of the limb. Thus this technique is very useful inrepairing soft tissue defect in the hand and can replace the classical fasciocutaneous flap, local muscle flap and even free flap.
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