괄루인 렉틴의 특성

김경순,최근호,박종옥,Kim, Kyung-Soon,Choi, Gun-Ho,Park, Jong-Ok 생화학분자생물학회 1994 한국생화학회지 Vol.27 No.5

Trichosanthis fructus Lectin (TFL) was partially purified by ammonium sulfate fractionation, heat treatment, DEAE-Sephadex ion exchange chromatography and Sephadex G-200 gel chromatography. The molecular weight of TFL was estimated by gel filtration and SDS-PAGE. The Molecular size of the lectin was determined to be 67,000 dalton, which was composed of two subunits having molecular weights of 34,000 and 35,000 dalton, respectively. The effect of sugars and metal ions on the hemagglutinating activity of lectin was also examined. The hemagglutinating activity of the lectin was inhibited by some sugars (${\beta}$-Lactose, ${\alpha}$-Lactose, D-(+)-Galactose) and metal ions ($Hg^{2+}$, $Cu^{2+}$, $Mg^{2+}$, $Ca^{2+}$, $Pb^{2+}$). 한국 중남부에 서식하는 다년생 박과 덩쿨식물인 하눌타리(Trichosanthes kirilowii) 씨앗인 괄루인(Trichosanthis fructus)으로부터 열처리, 황산 암모늄 분별침전, DEAE Sephadex 이온교환 크로마토그래피, Sephadex G-200 겔 크로마토그래피 등으로 렉틴을 정제하여 성질을 조사하였다. 렉턴은 2.3%의 수율로 60.4배 정제되었다. 겔 크로마토그래피로 측정한 분자량은 약 67,000 dalton, SDS-PAGE로 측정한 분자량은 34,000과 35,000 dalton의 두가지로 나타났으므로 이 렉틴은 dimer로 판단된다. Trichosanthis fructus 렉턴(TFL)은 galactose, ${\beta}$-lactose, ${\alpha}$-lactose 등의 당과 $Hg^{2+}$, $Cu^{2+}$, $Mg^{2+}$, $Pb^{2+}$ 등의 금속이온에 의하여 활성이 저해되었고 $40^{\circ}C$에서 15분간 유지시킨 결과 활성의 80%가 남았다.

닭 간의 Ornithine Aminotransferase 에 관한 연구

김경순,박종옥,박광숙 ( Gyung Soon Kim,Jong Ok Park,Kwang Sook Park ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.2

Ornithine aminotransferase was purified from chicken liver and several properties of the enzyme were studied. The purified enzyme appeared to be homogeneous on polyacrylamide gel electrophoresis with and without SDS. The molecular weight of the enzyme was estimated as 62,000 by SDS-polyacrylamide gel electrophoresis, and as 63,000 by gel filtration. The K_m values for ornithine and α-ketoglutarate were found to be 6.31 mM and 1.03 mM, respectively. The enzyme was inhibited either by high substrate concentrations or by p-chloromercuriphenylsulfonic acid. Glyoxylate, oxaloacetate and pyruvate could act as amino group acceptors, but they were much less effective than α-ketoglutarate. Optimum pH of this enzyme was found to be 7.3.

Bakers' Yeast를 이용한 m-Bromonitrobenzene 및 Nitrosobenzene의 환원반응

김경순,백운필,오성환,Kim, Kyungsoon,Baik, Woonphil,Oh, Sunghwan 대한화학회 1995 대한화학회지 Vol.39 No.10

방향족 니트로 화합물을 선택적으로 환원시켜 치환체가 있는 아닐린을 생성하는 것은 산업적 측면에서 볼때 의미있는 반응이다. 본 연구에서는 Bakers' Yeast-NaOH의 환원작용에 의하여 m-Bromonitrobenzene 의 니트로기가 선택적으로 빠르게 환원되어 치환체가 있는 아닐린 화합물이 형성됨을 확인하였고 특히 Nitrosobenzene이 수산화 나트륨이 없는 조건에서 Bakers' Yeast만의 작용에 의해 환원되어 아닐린을 생성함을 확인하였다. 또한 Nitrosobenzene의 Bakers' Yeast 환원에 미치는 저해제들의 효과 및 온도, pH의 영향 등을 조사하였다. Rapid and selective reduction of aromatic nitro compounds is of important for the preparation of amino derivertives in organic synthesis, particularly when a molecule has other reducible substituents. While Bakers' Yeast has been used for the enantioselective reduction of carbonyl compounds, little attention has been paid to the reduction of aromatic nitro compounds with Bakers' Yeast. Nitro group of m-bromonitrobenzene was selectively and rapidly reduced to corresponding amino derivative in good yield by Bakers' Yeast in basic solution. Furthermore, nitrosobenzene was rapidly reduced to aniline in good yield by Bakers' Yeast under neutral condition. In this paper, we wish to report a rapid and simple reduction of m-bromonitrobenzene and nitrosobenzene to the corresponding amino derivatives using Bakers' Yeast. And the effects of various agents, temperature and pH on the reduction will be discussed.

비유를 사용한 수업에서 학생들의 인지적.정의적 특성과 대응 이해 및 대응 오류 유형과의 관계

김경순,황선영,노태희,Kim, Kyung-Sun,Hwang, Sun-Young,Noh, Tae-Hee 대한화학회 2010 대한화학회지 Vol.54 No.1

이 연구에서는 비유를 사용한 '농도와 반응속도'에 대한 학습에서 학생들의 인지적 정의적 변인들의 상 하위 수준에 따른 대응 관계 이해도와 대응 오류 유형의 차이를 비교하고, 이 변인들과 대응 관계 이해도와의 관계를 조사하였다. 사전검사로 논리적 사고력, 시각적 심상화 능력, 비유 추론 능력, 자아 효능감, 인지욕구 검사를 실시하였다. 비유를 사용한 수업을 진행한 후, 친숙도 검사와 대응 관계 이해도 검사를 실시하였다. 연구 결과, 시각적 심상화 능력과 친숙도를 제외한 모든 인지적 정의적 변인의 수준이 상위인 학생들의 대응 관계 이해도 점수가 하위 학생들보다 통계적으로 유의미하게 높았다. 또한, 과잉 대응, 대응 불이행, 불가능한 대응, 인위적 대응, 부적절한 대응, 무분별한 대응, 비유물 속성 보유 등의 대응 오류 유형의 빈도가 학생들의 인지적 정의적 변인의 수준에 따라 다른 양상을 보였다. 학생들의 대응 관계 이해도 점수는 모든 인지적 정의적 변인들과 정적인 상관이 있었다. 다중 회귀 분석 결과, 과학 성취도, 논리적 사고력, 친숙도가 대응 관계 이해도에 대한 유의미한 예언 변인으로 나타났다. 이에 대한 교육적 함의를 논의하였다. In this study, we investigated the differences of mapping understanding and the types of mapping errors by the levels of students' cognitive/affective variables and the relationships between mapping understanding and these variables in learning 'concentration and reaction rate' with analogy. After administering the tests regarding logical thinking ability, visual imagery ability, analogical reasoning ability, self efficacy, and need for cognition as pretests, students learned with analogy. Then, students' familiarity and mapping understanding were examined. Analyses of the results revealed that the scores of the mapping understanding for the students with higher levels of all cognitive/affective variables except visual imagery ability and familiarity were significantly higher than those for the students with lower levels. The differences in the types of the mapping errors such as overmapping, failure to map, impossible mapping, artificial mapping, mismapping, rash mapping, and retention of a base feature were also found by the levels of students' cognitive and affective variables. The scores of students' mapping understanding were positively correlated with those of all cognitive and affective variables. The results of multiple regression analysis indicated that students' science achievement, logical thinking ability, and familiarity were significant predictors of mapping understanding. Educational implications of these findings are discussed.

모유수유와 분유수유에 따른 영아 장내 미생물 군집의 특징

김경순,신정,심지수,연수지,이평안,정문규,Kim, Kyeong Soon,Shin, Jung,Sim, JiSoo,Yeon, SuJi,Lee, Pyeong An,Chung, Moon Gyu 한국미생물학회 2019 미생물학회지 Vol.55 No.3

장 내에 존재하는 microbiome은 생활환경, 나이와 섭취하는 음식물에 따라 변하게 된다. 본논문에서는 모유를 섭취한 영아와 분유를 섭취한 영아들에 대한 장내미생물 군집의 변화를 비교하였다. 미생물의 군집변화를 차세대 염기서열 분석기법을 이용하여 분석하였으며 총 80개의 영아 분변을 통해 미생물 군집변화를 확인하였다. 모유그룹(BIG)과 분유그룹(FIG-A, FIG-B, FIG-C)간의 장내세균의 군집을 비교한 결과 BIG에서는 Actinobacteria 문이 전체 군집의 $74.22{\pm}3.48%$로 우점을 차지하였다. 분유그룹의 Actinobacteria 문을 비교하였을 때 FIG-A는 $73.46{\pm}4.12%$였지만 FIG-B와 FIG-C는 $66.52{\pm}5.80%$와 $68.88{\pm}4.33%$로 BIG와 FIG-A에 비해 낮은 비율을 보였다. 속(genus) 수준에서 살펴보면 Bifidobacterium이 전체 미생물군집에서 가장 높은 비율로 분포하고 있었으며 BIG가 $73.09{\pm}2.31%$로 가장 높았고 FIG-A, FIG-B 및 FIG-C는 각각 $72.25{\pm}4.93%$, $63.81{\pm}6.05%$ 및 $67.42{\pm}5.36%$를 차지하였다. Bifidobacterium 종의 경우 모든 그룹에서 Bifidobacterium longum이 우점을 차지하고 있었으며 BIG와 FIG-A가 전체 군집의 $68.77{\pm}6.07%$와 $66.85{\pm}5.80%$를 차지하였다. 이에 반해 FIG-B와 FIG-C는 각각 $58.94{\pm}6.20%$와 $61.86{\pm}5.31%$로 BIG와 FIG-A보다 낮은 비율로 분포하는 것을 분석하였다. FIG-A가 섭취한 분유는 Bifidobacterium의 선택적 증식이 우수하다고 알려진 갈락토올리고당, 갈락토실락토스, 시너지올리고당, 비피도올리고 및 장내균총개선소재를 혼합한 5-Bifidus factor 복합물이 포함되어 있다. 이와 같은 5-Bifidus factor라는 유산균에 대한 증식능이 우수한 프리바이오틱스를 혼합하였기 때문에 모유를 섭취한 영아의 장내에 존재하는 Bifidobacterium 속의 군집과 유사하게 나타난 것으로 판단된다. The intestinal microbiomes vary according to the factors such environment, age and diet. The purpose of this study was to compare the gut microbial diversity between Korean infants receiving breast-fed milk and formula-fed milk. We analyzed microbial communities in stool samples collected from 80 Korean infants using next generation sequencing. Phylum level analysis revealed that microbial communities in both breast-fed infants group (BIG) was dominated by Actinobacteria ($74.22{\pm}3.48%$). Interestingly, the phylum Actinobacteria was dominant in formula-fed infants group A (FIG-A) at $73.46{\pm}4.12%$, but the proportions of phylum Actinobacteria were lower in formulafed infants group B and C (FIG-B and FIG-C) at $66.52{\pm}5.80%$ and $68.88{\pm}4.33%$. The most abundant genus in the BIG, FIG-A, FIG-B, and FIG-C was Bifidobacterium, comprising $73.09{\pm}2.31%$, $72.25{\pm}4.93%$, $63.81{\pm}6.05%$, and $67.42{\pm}5.36%$ of the total bacteria. Furthermore, the dominant bifidobacterial species detected in BIG and FIG-A was Bifidobacterium longum at $68.77{\pm}6.07%$ and $66.85{\pm}4.99%$ of the total bacteria. In contrast, the proportions of B. longum of FIG-B and FIG-C were $58.94{\pm}6.20%$ and $61.86{\pm}5.31%$ of the total bacteria. FIG-A showed a community similar to BIG, which may be due to the inclusion of galactooligosaccharide, galactosyllactose, synergy-oligosaccharide, bifidooligo and improvement material of gut microbiota contained in formula-milk. We conclude that 5-Bifidus factor contained in milk powder promotes the growth of Bifidobacterium genus in the intestines.

Studies on the Ornithine Aminotransferase from Chicken Liver

김경순,박종옥,박광숙,Kim, Gyung-Soon,Park, Jong-Ok,Park, Kwang-Sook 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.2

닭 간에서 ornitine aminotransferase는 주로 mitochondrial fraction에 존재하였다. 이 효소를 열처리, ammonium sulfate fractionation, DEAE-cellulose ion exchange chromatography, Sephadex G-200 filtration, CM-Sephadex column chromatography를 이용하여 정제해서 disc polyacrylamide gel electrophoresis를 하였더니 하나의 단백질 띠가 얻어졌다. 이 정제된 효소를 이용해서 몇가지 성질을 조사해본 결과는 다음과 갈다. SDS-polyacrylamide gel electrophoresis에 의해 측정된 분자량은 62,000이었고 gel filtration에 의한 값은 63,000이었으므로 이 효소는 monomer로 존재한다고 생각되었다. L-ornithine과 $\alpha$-ketoglutarate에 대한 $K_m$ 값은 각각 6.31 mM, 1, 03 mM 이였으며, 이 효소는 기질의 농도가 높을 때 저해되었고 또한 3 mM p-chloromercuriphenylsulfonic acid를 가해주면 80% 저해되었다. glyoxylate, oxaloacetate, pyruvate도 L-ornithine보다는 못하지만 amino group acceptor로서 작용하였으며, 효소활성에 대한 최적 pH는 7.3으로 측정되었다. Ornithine aminotransferase was purified from chicken liver and several properties of the enzyme were studied. The purified enzyme appeared to be homogeneous on polyacrylamide gel electrophoresis with and without SDS. The molecular weight of the enzyme was estimated as 62,000 by SDS-polyacrylamide gel electrophoresis, and as 63,000 by gel filtration. The $K_m$ values for ornithine and $\alpha$-ketoglutarate were found to be 6.31 mM and 1.03 mM, respectively. The enzyme was inhibited either by high substrate concentrations or by p-chloromercuriphenylsulfonic acid. Glyoxylate, oxaloacetate and pyruvate could act as amino group acceptors, but they were much less effective than $\alpha$-ketoglutarate. Optimum pH of this enzyme was found to be 7.3.

난소암 치료에 대한 한의 임상 가이드라인

김경순,최홍식,김승모,유화승,Kim, Kyung-Soon,Choi, Hong-Sik,Kim, Seung-Mo,Yoo, Hwa-Seung 대한암한의학회 2016 大韓癌韓醫學會誌 Vol.21 No.2

Ovarian cancer is the tenth most common cancer in Korean female, and the third most common cancer of female reproductive organ after breast cancer and uterine cervical cancer. In spite of develop of conventional treatment, high modality of ovarian cancer comes from difficulty of an early diagnosis. Recent studies revealed that combining conventional and integrative medical treatment can reduce the adverse effect of surgical operation, chemotherapy and radiotherapy. Also it can improve survival rate, quality of life. However there isn't still a systemic clinical protocol for ovarian cancer in Korean medicine. This study will be helpful to establish clinical practice guidelines of Korean Medicine for ovarian cancer. And further studies on integrative ovarian cancer treatment are needed to build the clinical practice guidelines of ovarian cancer.

Adsorption Behaviors of Indomethacin on Cholestyramine Resin

김경순,Kim, Kyung-Soon 한국약제학회 1988 Journal of Pharmaceutical Investigation Vol.18 No.3

In vitro studies were performed on the interaction of indomethacin with cholestyramine, a hypocholesterolemic substance. Cholestyramine showed a marked affinity for indomethacin among tested acidic drugs and the intensity of adsorption was dependent on pH, temperature and sodium chloride. Moreover, the combination of indomethacin with some acidic drugs that formed complexes with cholestyramine, considerably inhibited the adsorption of indomethacin on the resin.

괄루인 렉틴의 특성

김경순,최근호,박종옥 ( Kyungsoon Kim,Gunho Choi Jong Ok Park ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.5

Trichosanthis fructus Lectin (TFL) was partially purified by ammonium sulfate fractionation, heat treatment, DEAF-Sephadex ion exchange chromatography and Sephadex G-200 gel chromatography. The molecular weight of TFL was estimated by gel filtration and SDS-PAGE. The Molecular size of the lectin was determined to be 67,000 dalton, which was composed of two subunits having molecular weights of 34,000 and 35,000 dalton, respectively. The effect of sugars and metal ions on the hemagglutinating activity of lectin was also examined. The hemagglutinating activity of the lectin was inhibited by some sugars (β-Lactose, α-Lactose, D-(+)-Galactose) and metal ions (Hg^(2+), Cu^(2+), Mg^(2+), Ca^(2+), pb^(2+)).

항암단 투여로 호전된 진행성 또는 전이성 담낭암 연속환자증례

김경순,유화승,정태영,이연월,조종관,Kim, Kyung-Soon,Yoo, Hwa-Seung,Jung, Tae-Young,Lee, Yeon-Weol,Cho, Chong-Kwan 대한한방내과학회 2010 大韓韓方內科學會誌 Vol.31 No.3

Objective : To investigate the therapeutic effects of Hang-Am-Dan(HAD) on gallbladder cancer patients. Method : We prescribed HAD three times a day(3,000-6,000 mg/day) during a treatment period of 8 and 34 months respectively. Computed tomography(CT) was taken to evaluate the therapeutic efficacy. Results : Two patients diagnosed with gallbladder cancer were recommended to go through chemotherapy. However, they refused the standard regimen due to their old age and the fear of side effects, and chose to go with Oriental medicine treatments instead. The patients were treated for 8 and 34 months respectively. Each patient showed stable disease(SD) state during the treatment period. Conclusion : According to these cases, it could be concluded that HAD may offer potential benefits in treating gallbladder cancer patients.