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신장기능영상용 방사성의약품 Bz - MAG3 ( Benzoylmercaptoacetylglycylglycylglycine ) 의 키트화 및 체내분포
고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),이동수(Dong Soo Lee),김영주(Young Ju Kim),정재민(Jae Min Jeong),장영수(Young Soo Chang),조정혁(Jung Hyuk Cho) 대한핵의학회 1996 핵의학 분자영상 Vol.30 No.3
N/A The MAG3 is a tubular excreting radiopharmaceutical for renal image. We synthe-sized benzoyl MAG3 (Bz-MAG3) and made a kit for labeling with Tc-99m. We checked the labeling effeciency of Tc-99m labeled MAG3 and biodistribution. Labeling efficiency was checked by TLC- SG (acetonitrile/H2O=2/1). After injecting of 1 mCi of Tc-99M-MAG3 to ICR-mice, Tmax(min), T1/2(min) were obtained in the renogram. Sequencial images (30sec, 2min, 5min, 10min, 15min, 20min) of TC-99m-MAG3 were compared with those of commercial Tc-99m-DTPA (Du Pont Merck Pharmaceutical Co.) kit. 1) The Rf value of synthesized Tc-99m-MAG3 was 0.78 and labeling efficiency was 97.5±1.9% (n=10). 2) The dynamic images of the Tc-99m-MAG3 were better than those of the Tc-99m-DTPA. 3) The Tmax(min.) and T1/2(min.) of Tc-99m-MAG3 (n=10) were 1.5±0.5 (left), 1.4±0.4(right), and 4.3±1.4 (left), 4.8±2.0 (right), respectivel. The Tmax(min.) and T1/2(min.) of Tc-99m-DTPA (n=7) were 2.7±1.6 (left), 2.7±1.6 right), and 3.8±1.7 (left), 4.5±2.7 (right), respectively. The quaility of image and labeling efficiency of the synthesized Bz-MAG3 kit were excellent, that it was supposed to be used in routine clinical work.
Iodin-131I OIH와 99mTc - MAG3의 약물동력학 및 체내 분포에 관한 비교 연구
고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),조보연(Bo Youn Cho),이동수(Dong Soo Lee),이범우(Bum Woo Lee),정재민(Jae Min Jeong),염미경(Mi Kyoung Yeom),김문혜(Mun Hye Kim),박경호(Kyung Ho Park),송세흠(Sae Heum 대한핵의학회 1990 핵의학 분자영상 Vol.24 No.1
N/A 99mTc-MAG, was synthesized, and biodistribution and blood clearance rate were compared with those of 131I-OIH in mice and rats respective]y. Finally renal image was obtained from a normal male volunteer before and after prescription of probenecid. The results obtained were as follows: 1) The uptalce of Tc-MAG, by kindey was higher than that of 131I-OIH in mice 10 mins after injection (n=6, p<0.05), but slightly lower uptakes were found in all organs (kindney, blood, stomach, mtestinge and liver) 2 hrs after injection. 2) For ' Tc-MAG, t1/2 a=2.4-+0.0 min, t1/2B=44.3+7.4 min, and blood clearance=3.4+0.5ml/min, and for 131I-OIH t1/2=1.8=0.2 min, t1/2=69.1+9.5 min, and blood clearance=1.3+O.l min were found in rats. 3) From the renogram of normal male volunteer, we could find that tmax=130 sec and t1/2=430sec before probenecid prescriptiori, and tmax 150-170sec and t1/2=810-1,170sec after probenecid prescriprion. From these results we concluded that Tc-MAG, can be used instead of 131I-OIH for obtaining renal irnage.
I-123 표지 지방산의 체내 분포 및 대사 ( I ) - < I-123 > 15-( p-iodophenyl ) pentadecanoic acid ( IPPA )
고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),이동수(Dong Soo Lee),정재민(Jae Min Jeong),장영수(Young Soo Chang),서용섭(Yong Sup Suh) 대한핵의학회 1998 핵의학 분자영상 Vol.32 No.1
N/A I-123 labelled fatty acids are suitable for investigation of regional myocardial metabolism, so they are on the clinical trial. However, the precise properties of these materials are not characterized yet. We have synthesized phenylpentadecanoic acid and labeled this compound with I-123. The purpose of this study was to examine the stability, biodistribution, metabolism and SPECT imaging of [I-123]15-(p-iodophenyl)pentadecanoic acid(I-123-IPPA) that we made. The stability test of I-123-IPPA in serum of rat, mouse and human showed no free I-123 after 1 hour. In biodistribution study in mice for various time intervals after injection(5, 10, 15, 30, 60 minutes), uptake in myocardium was 14.5%ID/g(5 min), and 1.9%ID/heart(5 min), while uptake in muscles was 2.6%ID/g(5 min). Myocardium to blood ratio and myocardium to lung ratio increased for 5 min after injection and then decreased rapidly. Chromatographic data of rat blood and urine showed that little PPA was found in blood and urine 15-20 min after injection. The myocardial I-123-IPPA SPECT images of a dog with myocardial infarction showed defects similar to those of Tc-99m-MIBI and F-18-FDG. These data suggest that I-123-IPPA is quite stable in vitro and shows favorable biodistribution in mice. SPECT imaging with I-123-IPPA demonstrated infarct zone as photon defect in dog model of myocardial infarction. I-123-IPPA may be used for the evaluation of fatty acid metabolism in clinical trials in Korea.
뇌혈류 영상용 방사성의약품 PRODD의 99mTc 표지 및 생체내분포
고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),이동수(Dong Soo Lee),정재민(Jae Min Jeong),조정혁(Jung Hyuck Cho),오승준(Seung Joon Oh),정수욱(Soo Wook Chung) 대한핵의학회 1995 핵의학 분자영상 Vol.29 No.3
N/A Tc-99m labeled PnAO(propylene amine oxime) derivatives have been widely studied as brain perfusion agents. We synthesized and characterized a PnAO derivative, (RR/SS/meso)-4,8-diaza- 3,9,dimethylundecane-2,l0-dione bisoxime(PRODD). Proton-NMR spectroscopy and thin layer chromatography(silence gel) were performed for its characterization PRODD was 1abeled with Tc- 99m using stannous chloride as reducing agent. The labeling efficiency was determined to be about 85%. Brain uptakes of 4.16±0.42 %ID/g and 3.24±0.13 %ID/g were found after l0min and 30min after intravenous injection. Brain-to-blood ratios were 1.17 and 0.75 at 10 and 30 minutes, which were lower than 1.3 and 1.9 of the ratios with commercial Tc-99m-HMPAO. Autoradiographs of rat brain sections showed the gray matter to white matter ratios of 1.13±0.10 at 30 min after intravenous injection, which was lower than 1.94±0.19 of commercial Tc-99m-HMPAO. With the above findings, we concluded that the lipophilic Tc-99m-PRODD complex was able to cross the blood-brain barrier, however the complex showed lower uptake than Tc-99m-HMPAO in mouse or rat brains. We Could suggest possibility that PRODD could be used as another Tc-99m chelator.
사람 대장암 세포주의 [ 18F ] fluorodeoxyglucose 섭취의 특징
고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),정재민(Jae Min Jeong),김채균(Chae Kyun Kim) 대한핵의학회 1997 핵의학 분자영상 Vol.31 No.3
N/A Cancer tissues are characterized by increased glucose uptake. 18F-fluorodeoxyglucose(FDG), a glucose analogue is used for the diagnosis of cancer in PET studies. This study was aimed to compare the glucose uptake and glucose transporter l(GLUT1) expression in various human colon cancer cells. We measured FDG uptake by cell retention study and expression of GLUTI using Western blotting. Human colon cancer cells, SNU-C2A, SNU-C4 and SNU-C5, were used. The cells were incubated with 1μCi/ml of FDG in HEPES-buffered saline for one hour. The FDG uptake of SNU-C2A,SNU-C4 and SNU-C5 were 16.8±1.36, 12.3±5.55 and 61.0±2.17cpm/μg of protein, respectively. Dose-response and time-course studies represent that FDG uptake of cancer cells were dose dependent and time dependent. The rate of FDG uptake of SNU-C2A, SNU-C4 and SNU-C5 were 0.29±0.03, 0.21±0.09 and 1.07±0.07cpm/min/μg of protein, respectively. Western blot analysis showed that the GLUT1 expression of SNU-C5 was significantly higher than those of SNU-C2A and SNU-C4. These results represent that FDG uptake into human colon cancer cells are different from each other. In addition, FDG uptake and expression of CLUT1 are closely related in human colon cancer cells.
F - 18 - FDG 감쇠보정 전신 PET을 이용한 표준섭취계수 추정과 매개변수 영상의 구성
고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),이동수(Dong Soo Lee),김영진(Yong Jin Kim),김경민(Kyeong Min Kim),정재민(Jae Min Jeong),곽철은(Cheol Eun Kwark) 대한핵의학회 1996 핵의학 분자영상 Vol.30 No.4
N/A Background and Purpose: Standardized uptake value(SUV) has been used as a quantitative index for differentiating benign and malignant tumors with F-18-FDG PET. In this study, we produced whole body parametric images of SUV(WBPIS) by body weight normalization, and validated the values by comparison with SUV's calculated with regional scans. Subjects and Methods: Whole body scans were followed by regional scans sequentiallly on 23 patients. In whole body study, transmission and emission scans were acquired for 2 minutes and 6 minutes for each bed position, respectively. In regional study, transmission and emission scan were acquired for 20 minutes. Measured and segmented/whole body studies. The effects of attenuation correction on SUVs were evaluated quantitatively using F-18 filled cylindrical phantom. The mean and peak SUVs obtained from WBPIS were compared with SUVs of the regional scans. Results: In phantom studies, with any method of attenuation correction using regional or whole body studies of phantom, SUVs were nearly consistent. In whole body scan, SUV obtained using measured attenuation correction method was a little higher than SUV of regional scan. SUV obtained using segmented/smoothed attenuation correction method as a little lower. In patient studies, WBPIS using segmented/smoothed attenuation correction method was much smoother and more readable. SUVs of WBPIS obtained with both methods of attenuation correction were well correlated with SUVs of regional scans(r=0.9). SUVs of WBPIS with measured attenuation correction method well 5% lower than SUVs of regional scans. SUVs of WBPIS with segmented/smoothed attenuation correction method were 10% lower than SUVs of regional scans. The differences of SUVs of WBPIS by the two attenuation correction methods were relatively small compared with the possible differences derived from biological characteristics of tumors. Conclusion: We concluded that WBPIS could be useful in the quantification of tumor as well as in localization of whole body lesions, which w
국산 항 백혈병 항체 ( 항 CALLA , 항 JL - 1 ) 의 동위원소 표지 및 면역학적 특성에 관한 연구
고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),이동수(Dong Soo Lee),박성회(Seong Hoe Park),정재민(Jae Min Jeong),소영(Young So) 대한핵의학회 1995 핵의학 분자영상 Vol.29 No.1
N/A Recently murine monoclonal antibodies have studied actively for radioimmuno-scintigraphy and radioinamunotherapy, especially on patients with leukemia and lymphoma. In this research, we studied radiolabeling and immunologic characteristics of two in-house anti-leukemic monoclonal antibodies(anti-CALLA & anti-J1-l antibodies) to make the basis tor their clinical application. Each antibody was radiolabeled successfully with 99mTc by pretargeting transchelation method and with 125I by Iodogen method. We also studied cell binding assay, Scatchard analysis and modulation phenomenon. 125I showed 90% labeling efficiency for each anti-body which was satisfactory, but 99mTc showed labeling efficiency below 70%, for which we need better labeling method, In cell binding assay, the immunoreactivity(IR) was low for 99mTc-labeled antibodies. Scatchard analysis showed satisfactory data for both binding affinity. The affinity constant and antibody binding sites per cell are around 109M-1 and 104, respectively. There was no modulation phenomenon in cases of 125I or 99mTc labeled antibodies. We expect that two anti-leukemic monoclonal antibodies may be useful in diagnosis and therapy for leukemia and lymphoma patients.
수술 후 암 재발 판정에 있어서 전신 F - 18 FDG - PET의 유용성
고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),이동수(Dong Soo Lee),강원준(Won Jun Kang),정재민(Jae Min Jeong),소영(Young So),곽철은(Cheol Eun Kwark),강순범(Soon Beum Kang),정희원(Hee Won Jung),김광현(Kwang Hyu 대한핵의학회 1997 핵의학 분자영상 Vol.31 No.3
N/A he purpose of this study was to evaluate the usefulness of whole body F-18 FDG PET scan for deceting postoperative recurrence of cancer. One hundred four cancer patients after operation were enrolled(l4 brain tumor, 15 head and neck cancer, 23 gynecologic cancer). Besides conventional images(CI) including CT and MRI, F-18 FDG PET scan was obtained on ECAT EXACT 47 scanner(Siemens-CTI), beginning 60 minutes after injection of 370MBq(10mCi) of F-18 FDG. Regional scan was also obtained with emission image. Transmission images using Ge-68 were carried out for attenuation correction in both whole body and regional images. Findings of PET and CI were confirmed by pathology or clinical follow up. The sensitivity and specificity of PET for detecting recurrence were 94% and 92%, respectively. Contrarily, the sensitivity and specific it of CI were 78% and 68% CI results were negative and PET results were positive in 11 cases. The biopsy or clinical follow-up of those cases confirmed recurrence of tumor. False negative cases of CI were frequent in patients with gynecologic cancers. Also we measured the Serum concentration of tumor markers in patients with gynecologic cancer(CA125), thyroid cancer(thyroglobulin), and colorectal cancer(CEA). The sensitivity and specificity of tumor markers were 71% and 84%, respectively. We conclude that F-18 FDG PET can be used valuably in detecting recurrent foci of a wide variety of malignancy compared to conventional diagnositic methods.
Miniaturized Chromatography에 의한 99mTc - 표지 방사성의약품의 정도 관리
고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),정재민(Jae Min Jeong),염미경(Mi Kyoung Yeom),진광호(Kwang Ho Jin),조규진(Kyu Jin Cho) 대한핵의학회 1990 핵의학 분자영상 Vol.24 No.1
N/A The general use of a number of Tc-99m-labelled compounds makes the need for good routine quality control procedures, especially the labelling efficiency measurements. The purpose of these study were to measure the labelling efficiency of Tc-99m-labelled MDP, DTPA, Tin colloid, and Antimony sulfide colloid using miniaturized paper and instant thin layer chromatography. The chromatographic systems include whatman 3 MM paper and acetone, Gelman ITLC-SG and 0.9% sodium chloride. The chromatographic strips are miniaturized (1×10 cm), marked and numbered. Labelling efficiencies of Tc-99m-labelled DTPA and Tin colloid were above 98.0%. Tc-99m- labelled Antimony sulfide colloid was 90.0%. And labelling efficiency of Tc-99m-labelled MDP was 89.0% Coefficient variance of Tc-99m-labelled MDP, DTPA, Tin colloid, and Antimony sulfide colloid were 5.14%, 2.06%, 1.82% and 4.90%, respectively. We found that this miniaturized chromatographic quality control was simple and reliable.
항 NCA - 95 단일클론항체의 99mTc 표지 키트 제조 및 특성 연구
고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),이동수(Dong Soo Lee),정재민(Jae Min Jeong),김채균(Chae Kyun Kim),홍미경(Mee Kyoung Hong),최석례(Seok Rye Choi),이용진(Yong Jin Lee) 대한핵의학회 1996 핵의학 분자영상 Vol.30 No.4
N/A The previous monoclonal antibody labeling method for bone marrow immunoscintigrapy was complicated and laborious for clinical application. Also it showed a relatively low labeling efficiency. To improve this procedure, we compared several direct labeling methods of Tc-99m. 1) The labeling efficiency in the method using gluconate as a transchelator was low (40-70%), but immunoscintigraphy using this radiotracer produced a clear image. 2) To improve labeling efficiency, β-mercaptoethanol was removed after reduction. The labeling efficiency was improved up to 70-80%, but the radioactivity of the blood pool was high. 3) The higest labeling efficiency (〉90%) and best quality images could be obtained by using MDP as a transchelating agent. It did not require additional procedures for separation of labeled antibodies. The immunoreactivity of this antibody was 60%. Residual MDP which can be taken up by the bone could be removed by PD-10 column. The reduced antibodies were stable with a high labeling efficiency (〉90%) for up to 47 days by deep freezing. We concluded that the improved procedure for Tc-99m labeling of anti-NCA-95 monoclonal antibody using MDP as a transchelating agent will be a simple and useful method for clinical application.