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Alkaline protease 분비세균의 분리동정 및 생성조건
陳翼烈,李貞淑 慶北大學校 1990 論文集 Vol.49 No.-
An alkalophilic soil isolate from the suburbs of Taegu city was identified as a faculatively anaerobic rod Aeromonas sp. JS-66, which has secreted an alkaline protease in the alkaline medium containing Na_2CO_3. The optimum culture condition for production of the alkaline protease by this strain JS-66 was as follows; 1.0% sucrose, 0.5% yeast extract, 0.5% polypeptone, 0.1% K_2HPO_4, 0.02% MgSO_4·7H_2O, 0.7% Na_2CO_3, pH 9, 30℃ and 20 hr.
호알카리성 Aeromonas 속 균주가 생성하는 Alkaline Protease의 정제 및 특성
陳翼烈,李貞淑 慶北大學校 1990 論文集 Vol.49 No.-
The alkaline protease from the best-cultured broth of Aeromonas sp. JS-66 was purified about 71 folds with the yield of 1.7% by ammonium sulfate precipitation, dialysis, DEAE-cellulose ion exchange chromatography and repeated gel filtration on Sephadex G-150. Optimal temperature and pH for activity of the purified enzyme were 50℃ and pH9.5. This enzyme was stable to 60℃ and within pH7 to 11. This enzyme was inactivated by Cu^2+, Zn^2+, Fe^2+ and EDTA, but activated by Mn^2+. The molecular weight of the enzyme was estimated about 24,000 by the SDS PAGE analysis.