한국산차수(韓國産茶樹)의 동해방제(凍害防除)에 관한 연구 : 약제산포법(藥劑散布法)과 피복법(被覆法)을 중심으로 Chiefly by Spraying Chemicals on the Leaves and Covering the Plants

김재생 경상대학교 경남문화연구소 1984 慶南文化硏究 Vol.7 No.1

In order to investigate effective method of the prevention of Frost-damage in the korean tea-plant, We sprayed plant-growth-regulators, NAA and Kinetin and MH and Gibberellin etc. on the leaves according to opportunity and density and established covering-materials according to material, and month, and so we could see the following results. 1. The districts were MH 1,000ppm and MH 2,000ppm were sprayed were to on extent of 24% more effective in reducing the Frost-damage than the districts where no chemicals were sprayed. 2. The most effective method of spraying chemicals was by doing at the beginning of passing the winter. 3. The district where Gibberellin 200ppm was sprayed had a reverse effect of 20% compared with that not sprayed. 4. The spraying of MH in October was more effective in promoting the persistency and preventing the defoliation of the seed leaves. than that of NAA. 5. In case Kinetin and MH were together sprayed, we could see some effect in the less densely sprayed district but not in the district where the highly densed Kinetin 500ppm and MH 2,000ppm were compoundely sprayed. 6. In the repeated spraying of chemicals in the sprouting-season, we could see the more effect in preventing Frost-damage caused by retarded-sprouting in proportion to their density and the breadth of district where chemicals were sprayed. 7. The district where Vinyl and Bamboo-blind-screen were together covered in september was to an extant of 20% more effective in preventing Frost-damage than that where only one covering material was covered.

조직배양에 의한 국산다 (國産茶) ( 다수 ) (茶樹) 의 증식에 관한 연구

김재생 ( Jai Saing Kim ) 한국산림과학회 1986 한국산림과학회지 Vol.75 No.1

In order to contribute to the Korean tea-plant culture and tea industry by means of increasing the production of tea-plants, I have performed the tissue culture of the organs of the anther, leaf and stem. As for the culture-material, I have used the anther of tea (Thea sinensis) at the tetrad uninucleate microspore stage and used medium of modified Murashige and Skoog as the basal medium supplemented with the growth regulators of NAA and 2, 4-D, yeast, kinetin and others at various concentrations. As for the handling of material, I have followed the common methods of sterilization and microtoming and paraffine imbedding method and observed systematically periodic changes of the microspores in culture. I have divided the leaf, stem and root into segments and sterilized them and used the modified Murashige and Skoog as the basal medium and observed the differentiation of roots and callus and the results are as follows. 1. In case of anther, I have found 2n callus was found in 30 out of 100 segments in M2 medium. 2, The differentiation of roots appeared in 24.5% of total leaf segments cultured and in 50.5% of stem and in 43.9% of root. 3. When the differentiation of stem in different parts was observed, the most frequent differentiation was found in the second part of all the 4 parts. 4. The most frequent formation of callus was noticed from the anther-walls in case of anther culture and from the veins in case of leaf culture. It is concluded that the seedlings of tea-plant could be multiplied most by means of tissue culture of the second part of the tea-plant stem and reduction in the expenditures of tea-plant propagation was possible through tissue culture.

한국산 다수의 (茶樹) 엽수병방제에 (葉銹病防除) 관한 연구

김재생,최재식 ( Jai Saing Kim,Jai Sik Choi ) 한국산림과학회 1987 한국산림과학회지 Vol.76 No.4

1. The hair cells on adaxial surface turned out to be penetration way of Gleosporium Thea sinensis in tea plant, 2. The most parts of Gleosporium Thea sinensis generated in tea plant were young leaves with first-fifth leaves from tip of shoot. 3. The proper temperature for spore germination on leaf is 25-27℃, soaked by water for 12 hours. 4. For prevention of Gleosporium Thea sinensis the drug-spay in tea plant would be the most effective when sprayed at this temperature range, and the control of Gleosporium Thea sinensis would be possible through selection of tea plant with few hairs. 5. The treatment of bordeaux mixture to prevent growth of conidiospore was 48.3% more effective than in control plot which were not sprayed. 6. The effect of sprayed bordeaux mixture decreased to about 28.5% after one week of spray.

지리산 죽류의 (竹類) 유관속초에 (維管束초) 의한 형태학적 연구

김재생 ( Jai Saing Kim ) 한국산림과학회 1977 한국산림과학회지 Vol.34 No.1

I have investigated and compared the morphology of vascular bundle shown in the section of culm wall of bamboo trees growing on Mt. Jiri which were classified by Grosser and Liese with their methods of morphological classification. The results obtained were as follows: 1. It was shown that there are no b.g.i. types of bamboo classified by Grosser and Liese among the bamboo trees on Mt. Jiri (Phyllostachys and Sasa). 2. As for the thickness of the culm wall in the culm, it was shown that the culm wall of the Phyllostachys becomes thinner in proportion to its nearness to the upper part of the tree, but no distinctive difference appeared in the Sasa. 3. The c, d, and e types of Sasa were the same as those of the Phyllostachys, but there was a vascular bundle type of the a` type, which was quite different from that of the Phyllostachys. 4. It was shown that the a`, d, and e types of Sasa were distributed in a zone less than 500m above sea level, but no a` type was distributed in the high mountain area except for the c,d and e types which ranged from 600m to l000m above sea level. Such facts mean that the vascular bundle sheath has changed in quantity because of the height of mountain. 5. In general, as compared with the Phyllostachys, the Sasa (types a, c, d and e which included a new type a) have fewer vascular bundles. 6. Considering the above results, it is thought that not by the current Sasa classification method based on observation of the the study of Sasa form the outside, but by a new method of classification based on the aspect of the physiological construction as seen from the inside wall is advanced. I believe this new method of classification to be a first step towards an epoch-making methodological advance and encourage the further study of it.

한국산 석이의 (石耳) 생육환경과 조직배양에 관한 연구

김재생 ( Jai Saing Kim ) 한국산림과학회 1989 한국산림과학회지 Vol.78 No.4

The objectives of this study were to investigate the growth of Gyrophora esculanta and to establish a method of tissue culture of the plant. The results obtained were as follows : 1. The Gyrophora esculanla was found growing mostly on the rock slopes of 722 m to 1915 min elevation on mountains in Korea. 2. Trees growing in the vicinity of the G. esculanta scere mainly Qzrerczrs spp., Pinus thzenbezgii, Acer spp. and Leapedeza spp, Especially Quercus spp. was found growing in all of the study site. 3. The average Length of the rock slopes with G, e.cculartta growing on was 14 m and their aspects were mostly south. 4. The G. esculanta were found growing on rocks of Crystalline Schist, Quartz, Liparite, Granite, ete. Particularly they were mostly found on granites. The gradient of the rock slopes was in the range of 22-90 degrees. 5. The mean number of individuals of G. escaclanta per one rock slope ranged from 14 at Mt . Buk.han to 70 at Mt. Jrri. Their mean diameter ranged from 1.8cm at Mt. Munsu to 4.6cm at Mt, Sokri. 6. The average percentage of G. esczrlanta with fruit body was 17.6%. The highest value was found at Mt. Cheonhwang (24.0%) . 7. When the 100 segments of rhizoid of Gyruphora escaclarrta cultured in Detmer`s medium supplemented with kinetine 5㎎/ℓ and 2, 4-D 3㎎/ℓ, n callus of microspore origins were induced from about 20% of the segments. As the induced n callus was transplanted on the six different types of rocks, it was observed that the juvenile G. esculanta grew best on granite and the development rate of G. esczdanta on the granite was about 55%.

대나무류의 유관속초에 (維管束초) 의한 형태학적 연구

김재생 ( Jai Saing Kim ) 한국산림과학회 1975 한국산림과학회지 Vol.25 No.1

Among the many species of bamboo, it is well known that the dwarf-type is widely distributed in the tropical regions, and the slender type in temperated zone, In the temperated zone the trees have extensively differentiated into one hundred species in 50 genera. In many oriental countries, the bamboo wood is being used as a material for construction and for the manufacture of technical instruments. The bamboo shoot is also regarded as a good and delicious edible resource. Moreover, recent medical investigation verifies that the sap of certain species of the bamboo is an antibiotic effect against cancer. Fortunately, it is very easy to propagate the bamboo trees by using cutting from southeastern Asian countries. This important resource can further be used as a significant source of pulp,, which is becoming increasingly important. The classification system of this significant resource has not been completely established to date, even though its importance has been emphasized. Initiated by Canlevon Linne in the 18th century, a classification method concerning the morphological characteristics of flowers was the first step in developing a classification. But it was not an easy task to accomplish, because this type of classification system is based on the sexual organs in bamboo trees. Because the bamboo has a long life cycle of 60-120 years and classification according to this method was very difficult as the materials for the classification are not abundant and some species have changed, even though many references related to the morphological classification of bamboo trees are available nowadays. So, the certification of bamboo trees according to the morphological classification system is not reasonable for us. Consequently, the classification system of bamboo trees on the basis of endomorphological characteristics was initiated by Chinese-born Liese. And classification method based on the morphological characteristics of the vascular bundle was developed by Grosser. These classification methods are fundamentally related to Holltum`s classification method, which stressed the morphology of the ovary. The author investigated to re-establish a new classification method based on the vascular sheath. Twenty-six species in 11 genera which originated from Formosa where used in the study. The results obtained from the investigation were somewhat coordinated with those of Crosser. Many difficulties were found in distinguishing the species of Bambusa and Dendrocalamas. These two species were critically differentiated under the new classification system, which is based on the existence of a separated vascular bundle sheath in the bamboo. According to these results, it is recommended that Babusa divided into two groups by placing it into either subspecies or the lower categories. This recommendation is supported by the observation that the evolutional pattern of the bamboo thunk which is from outward to inward. It is also supported by the viewpoint that the fundamental hypothesis in evolution is from simple to complex. There remained many problems to be solved through more critical examination by comparing the results to those of the classification based on the sexual organs method. The author observed the figure of the cross-sectional area of vascular trunk of bamboo tree and compared the results with those of Grosser and Liese, i.e. A, B₁, B₂, C, and D groups in classification. Group A and B₂ were in accordance with the results of those scholars, while group D showed many differences, Grosser and Liese divided bamboo into $quot;g$quot; type and $quot;h$quot; type according to the vascular bundle type; and they included Dendrocalamus and Bambusa in Group D without considering the type of vascular bundle sheath. However, the results obtained by the author showed that Dendrocalamus and Bambusa are differentiated from each other. By considering another group, $quot;i$quot; identified according to the existence of separated vascular bu

Sasa 와 Bambusa 속의 유관속초형에 (維管束초型) 의한 비교연구

김재생 ( Jai Saing Kim ) 한국산림과학회 1978 한국산림과학회지 Vol.39 No.1

The morphological characteristics of vascular bundle sheath occurring on the culm wall were investigated by using many species of Bambusa arid Sasa in order to test new classification method for endomorphological charateristics of Bamboos. The results obtained were as follows. 1. As for the thickness of the culm wall in the culm, it was shown that the culm wall of the Bambusa becomes thinner in propertion to its nearness to the upper part of the tree, but no distinctive difference appeared in the Sasa. 2. It was shown that many species of Bambusa has a,b types but the Sasa had a` type and had a,b types. 3. It was shown that many species of Bambusa had e,h, and i types but the Sasa had not them and both of species had not f type. 4. It was shown that many species of Bambusa had c,d,e, and g types, but the Sasa had c,d, and e types and had not g type. 5. In the classification of Bambusa and Sasa, The method of the physiological classification was more effective than test of external observation, and it will encourage further study.

한국산다수의 (茶樹) 내한성에 (耐寒性) 관한 연구 특히 지역별 엽형태와 내한성을 중심으로

김재생,김창호 ( Jai Saing Kim,Chang Ho Kim ) 한국산림과학회 1981 한국산림과학회지 Vol.53 No.1

This study was aimed at expanding the tea plant culture in Korea to further north, and the leaf-form and the cold resistance of the tea plants selected form 7 districts(around Korea) were investigated. 1. The length of tea leaf is 5.1cm to 8.4cm, the width of the leaf is 2.3cm to 3.6cm, but the area of tea leaf in Mt. Mudung is 26.88㎠ and that is the widest of all. 2. The tooth number of the tea leaf in 43 to 73 but the number of Mt. Borim is remarkably number of all. The tooth number is increased or decreased in proportion to the leaf width and to the leaf length. 3. The lateral vein number is generally 13 to 19. The vein number of Mt. Borim is especially number of all. That is also increased or decreased in proportion to the leaf width and to the leaf length. 4. In general, the number of leaf formation index is 2 to 3, and ablong. 5. In general, those which come upper lands are remarkably small in length and width of the tea leaf and those which come from level lands are large. 6. All kinds of tea plant which is growing in Korea, area, are the same as those imported from China : Thea sinensis Linne var. Bohea. 7. I supposed that the external form of tea plant has a little changed by geography or climate for many a long day, since the tea plant had been transplanted in Mt. Samsin. 8. In the treatment of low temperature and duration of vernalization of their plants, those selected from Mt. Mudung and Mt. Hwaun were the coldest resistance, those from Mt. Samsin and Mt. Borim were medium and those from Mt. Joge and Nursery were codl sensitive. 9. The critical temperature of the tea plant from Mt. Mudung, Mt, Hwaum, Mt, Samsin and Mt, Borim was about -10℃, and that from Mt. Joga and Nursery was about -5℃. The critical temperature of frost injury of all tea plants in this experiments was -15℃ 10. In spite of increasing the vernalization time, the critical temperature was not effected, but the treatments over critical temperature were increased their frost injuries. Based on these results, the coldest resistance, Mt. Mudung tea plant, was considered expanding their culture to further north improvement yields of the tea plants in Korea

Prunus mume S . et Z . 외 삼종의 약배양에 관한 연구

김재생 ( Jai,Saing Kim ) 한국산림과학회 1976 한국산림과학회지 Vol.31 No.1

When haploid plant would be appeared by the anther culture, the large quantity of young plant multiplied maternal inheritance and the same pure line rapidly in the short length of time, which will be effected to cut down much expences efforts and time for the production of seeds or seedlings. Therefore, the development of the technique for this would be much profited in the country industry. In the late of a few years studies were early attempted in this field, but up this time there were a few success of plants only and none of perennial plant In this status of the country condition required earnestly for the development of the green industry, this researcher attempted to culture the anther of late uninucleate microspore or early binucleate microspore of the Prunus mume and other three psecies, economic trees estimated specially economic, on the place of Modified Murashige and Sloog`s medium supliment with Kinetine, 2.4-D, and N.A.A for inducing haploid plants. The obtained results were as follows: 1. 2,000 nathers were cultured and there were shown that 2N callus in Prunus mume had 82 as 4.1%, 2N callus in Prunus tomentosa 15 as 0.8%, 2 N callus in Prunus salisina 75 as 4% 2. N callus had shown 40 ad 2% from Prunus armeniaca var. ansu only, and the other trees showed all 2N callus. 3. Callus had appeared in every tree but 2N callus appeared was all filaments and there showed from only connective tissue N callus appeared was all from anther locule inside. 4. Then Prunus armeniaca var. ansu only was not callus of somatic anther tissue origin, but as there was callus origined from microspore which was changed in to swollen microspores or polynucleate microspores, it was certained to need haploid plan

일본의 다업사정과 (茶業事情) 연구체제 조사보고

김재생 ( Jai Saing Kim ) 한국산림과학회 1972 한국산림과학회지 Vol.16 No.1