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거대세포바이러스 조기항원 단백질에 대한 단세포군 항체 제조에 관한 연구
박지호,손영모,박규현,이원영,Park, Ji Ho,Sohn, Young Mo,Park, Kyu Hyun,Lee, Won Young 대한소아감염학회 1999 Pediatric Infection and Vaccine Vol.6 No.2
목 적 : human CMV가 생성하는 분자량 72,000 dalton의 immediate early protein(p72)과 반응하는 단세포군 항체를 국내에서 분리된 CMV 야생주로 직접 제작하여 진단에 사용해 보기 위한 것이다. 방 법 : 정상 인체 섬유아세포(Foreskin 유래)를 10% 우태아혈청이 함유된 Eagle' s minimal essential medium(MEM)에서 배양하여 사용하였으며 마우스 골수종세포는 P3X63 Ag8.653(ATCC, Mary land USA)을 $5{\times}10^5/ml$ 세포수를 유지하도록 배양하였다. 사용된 항원은 선천성 CMV 감염아의 소변으로부터 검출한 CMV(KJHJ90)를 대량 증식시켜 사용하였다. 생후 6~8주된 Balb/c마우스의 복강내에 CMV 항원을 1주일 간격으로 4회 주사한 후 면역시킨 마우스 비장에서 얻은 임파구와 형질세포종세포로 최적발육기의 P3X63-Ag8.653을 사용하여 융합하였다. 융합세포를 배양한 후 간접면역형광항체법을 이용하여 항체가가 높은 것을 골랐다. 결 과 : 생산된 28종의 단세포군항체 중 그림 1과 같이 LPC12와 LPC23클론이 AD169에 감염된 세포의 핵에 특히 강하게 반응하였다. 정제된 AD169를 SDS-PAGE한 후 Western blotting을 하여 생산된 단세포군항체가 반응하는 항원의 분자량을 확인하였으며 그 중 LPC12와 LPC23클론에서 생성된 단세포군항체는 약 72KDA의 항원과 특이적으로 가장 강하게 반응하였다. 결 론 : 본 연구에서 제작된 LPC12와 LPC23 클론에서 생성된 단세포군 항체를 이용하여 선천성 CMV감염으로 확인된 소아의 소변을 이용하여 CMV AD 169 주 유래 항체(P63-27)과 비교한 DEAFF 검사상 동일한 결과를 확인한 바 LPC12와 LPC 23 클론 항체는 AD 169(P63-27)과 같이 CMV 감염의 조기진단에 사용할 수 있을 것으로 사료된다. Purpose : The purpose of this study is to make and use monoclonal Ab which reacts with CMV major immediate early(${\alpha}$) protein(p72). Methods : Normal human fibroblast(Foreskin derived) was cultured in Eagle's minimal essential medium(MEM) containing 10% cowfetus serum and mouse chondroblast was cultured in P3X63 Ag8.653(ATCC. Maryland USA) to maintain $5{\times}10^5/ml$ cell counts. CMV(KJHJ90) from congenital CMV infected infant's urine was multiplied and used for Ab making. CMV Ag was injected 4 times, 1 week interval into the peritoneal space of 6~8 weeks old mice. And then lymphocyles and fibroblasts taken from spleen were obtained and conjugated. After the conjugated cell cultured, we chose the cell that had high Ab titer using indirect immunofluerescent method. Results : Among the 28 monoclonal antibodies obtained LPC12 and LPC23 reacted highly with nucleus of AD169 infected cell. Purified AD169 after SDS-PAGE, molecular weight of Ag, which reacted with purified monoclonal Ab, was obtained using Western blotting. Monoclonal Ab of LPC12 and LPC23 clone reacted most highly with 72 kd Ag. Conclusion : LPC12 and LPC23 clonal Ab with AD 169(P63-27) is useful on early diagnosis of CMV infection.
FlexRay 네트워크 시스템을 위한 FIBEX 자동 생성 알고리즘에 관한 연구
박지호,이석,이경창,Park, Ji-Ho,Lee, Suk,Lee, Kyung-Chang 대한임베디드공학회 2013 대한임베디드공학회논문지 Vol.8 No.2
As vehicles become more intelligent for safety and convenience of drivers, in-vehicle networking systems such as controller are network (CAN) have been widely used due to increasing number of electronic control unit (ECU). Recently, FlexRay was developed to replace CAN protocol in chassis networking systems, to remedy the shortage of transmission capacity and unsatisfactory real-time transmission delay of conventional CAN. However, it is difficult for vehicle network designers to calculate platform configuration registers (PCR) and determine a base cycle or slot length of FlexRay. To assist vehicle network designers for designing FlexRay cluster, this paper presents automatic field bus exchange format (FIBEX) generation algorithm from CANdb information, which is de-facto standard database format for CAN. To design this program, structures of FIBEX, CANdb and relationship among PCR variables are analyzed.
Sn-1.7Bi-0.7Cu-0.6In 솔더의 특성 연구
박지호,이희열,전지헌,전주선,정재필,Park, Ji-Ho,Lee, Hee-Yul,Jhun, Ji-Heon,Cheon, Chu-Seon,Jung, Jae-Pil 대한용접접합학회 2008 대한용접·접합학회지 Vol.26 No.5
Characteristics of Sn-1.7%Bi-0.7%Cu-0.6%In (hereafter, SBIC) lead-free solder was investigated in this study. The results from SBIC were compared to other lead-free solders such as Sn-3.5%Ag-0.7%Cu (hereafter, SAC), Sn-0.7%Cu (hereafter, SC), and lead-bearing Sn-37%Pb (hereafter, SP) alloy. Tensile properties of bulk solder, wettability, spreading index, bridge and dross were evaluated. As experimental results, tensile strength and elongation of SBIC was 62.5MPa and 21.5%, respectively. The tensile strength was comparable to that of SP solder. The wetting time of SBIC was 1.2 sec at $250^{\circ}C$, and its wetting properties including wetting force were as good as the SAC alloy. However, wettability of the SC was not so good as the SBIC and SAC. The spreading index of SBIC at $250^{\circ}C$ was 71 %, and it was similar level to those of SAC and SC solders. Bridging was not found for all solders of SBIC, SAC and SC in the range from 240 to $260^{\circ}C$. In dross test at $250^{\circ}C$ for an hour, the amount of dross produced from SBIC was about 57% compared to that from SAC.
예측 기법을 이용한 drive-by-wire 시스템의 결함 허용 시스템 개발
박지호(Ji Ho Park),김만호(Man Ho Kim),이석(Suk Lee),이경창(Kyung Chang Lee) 한국자동차공학회 2011 한국자동차공학회 지부 학술대회 논문집 Vol.2011 No.10-2
As many systems depend on electronics, concern for fault tolerance is growing rapidly. For example, a car with its steering controlled by electronics and no mechanical linkage from steering wheel to front tires (steer-by-wire) should be fault tolerant because a failure can come without any warning and its effect is devastating. In order to make system fault tolerant, there has been a body of research mainly from aerospace field. This paper presents the prediction based fault tolerant structure that can remove most erroneous values. In addition, several numerical simulation results are given where the suggested system outperforms well-known average and median voters.
박지호(Ji-Ho Park),임상길(Sang-Gil Lim),정대원(Dae-Won Jeong),이상훈(Sang-Hoon Lee),박성준(Sung-Jun Park) 전력전자학회 2011 전력전자학술대회 논문집 Vol.2011 No.7
유도가열의 경우 고효율, 자동화, 에너지절약, 적정온도제어 등을 하기 때문에 작업환경의 개선 및 접근성에 대한 많은 이점이 있어 널리 이용되고 있다. 최근 공진특성을 이용한 고효율화를 이룬 전력변환기에 대한 연구가 활발히 이루어지고 있다. 공진을 이용한 유도가열의 경우 인덕턴스와 커패시턴스의 공진점을 추출해야하는데 작업자의 환경이나 코일의 재질 등이 균일하지 않으므로 매번 공진점을 추종해야하는 단점이 있다. 따라서 본 연구에서는 자동으로 공진점을 추적하는 알고리즘을 제안하였고 실험을 통하여 이를 검증하였다.
박지호(Ji-Ho Park),이상덕(Sang-Duk Lee),정태영(Tae-Young Jyung),정기석(Ki-Seok Jeong),백영식(Young-Sik Baek),서규석(Gyu-Seok Seo) 대한전기학회 2011 전기학회논문지 Vol.60 No.5
This paper presents a new method of local voltage control to achieve coordinative control among UPFC(Unified Power Flow Controller) and conventional reactive compensation equipments , such as switched-shunt and ULTC(Under-Load Tap Changing) transformer. Reactive power control has various difficult aspects to control because of difficulty of system analysis. Recently, the progress of power electronics technologies has lead to commercial availability of several FACTS(Flexible AC Transmission System) devices. The UPFC(Unified Power Flow Controller) simultaneously allows the independent control of active and reactive power flows as well as control of the voltage profile. When conventional reactive power sources and UPFC are used to control system voltage , the UPFC reacts to the voltage deviation faster than the conventional reactive power sources. Keeping reactive power reserve in an UPFC during steady-state operation is always needed to provide reactive power requirements during emergencies. Therefore, coordination control among UPFC and conventional reactive power sources is needed. This paper describe the method to keep or control the voltage of power system of local area and to manege reactive power reserve using PSS/E with Python. The result of simulation shows that the proposed method can control the local bus voltage within the given voltage limit and manege reactive power reserve.