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우기종저(牛氣腫疽)의 개량보체결합반응(改良補體結合反應)에 관한 연구(硏究)
서부갑,Seo, Boo Kap 대한수의학회 1972 大韓獸醫學會誌 Vol.12 No.1
Studies on modified complement fixation (MCF) test of antiblackleg bovine serum were made and the results obtained were summarized as followings. 1. The most satisfactory antigen for the MCF test among various materials studied was found to be the vegetative cells of Cl, chauvoei grown in cooked meat medium (CMM) containing 0.5mM l-(alpha) alanine and 0.1mM manganese. The antigen was prepared by inoculating the spores of Cl. chauvoei, heated at $70^{\circ}C$. for 30 minutes, into the CMM followed by incubation at $37^{\circ}C$. for 15 hours. 2. An active component contained in the factor serum of fresh normal rabbit serum was found to be C'4 fraction. It was also shown that, furthermore, DEAE cellulose sieved C'4 fraction of the factor serum enhanced antibody titer and the highest antibody titer was resulted by the addition of 0.03 ml, of the factor serum to each tube. 3. More than four fold increases of antibody titer, in antiblbckleg bovine serum-antigen system, was made with the MCF test than that with the direct complement fixation test. 4. The MCF antibody titer of cattle vaccinated against blackleg was 128 until seven month and 64 for five months thereafter.
"어형 돈단독균의 분리상황"과 가축에 대한 그의 병원성과의 관계
서부갑,seo Bu gap 대한수의사회 1964 대한수의사회지 Vol.8 No.2
For the purpose of researching the Erysipelothrix rhusiopathiae(E.r) organisms isolated from fish body surface and pathogenicity of domestic animals, I made practical isolation tests of the E. r. organisms for market fishes(Sea-fishes) at the market, and