조직배양에 의한 반하〔Pinellia ternata(Thunb.) Briet〕의 대량번식에 관한 연구

崔定植,羅義植 韓國作物學會 1986 Korean journal of crop science Vol.31 No.1

반하〔Pinellia ternata(Thunb.) Briet〕의 조직을 기내배양하여 callus 유기, 기관분화 및 자구를 증식시키는데 적합한 배지와 배양부위를 선정코저 Mur-ushige & Skoog 배지에 2,4-D와 kinetin의 첨가량을 달리하여 검토하였고 또한 배양온도 및 배지의 pH 영향을 조사하였던 바 그 결과를 요약하면 다음과 같다. 1. Callus 유기는 배양 5~7일 후에 엽조직 및생장점조직의 절단면에서 관찰할 수 있었으며 2,4-D 2.0mg/ +kinetin 0.2mg/ 을 첨가한 구에서 callus의 형성 및 생장이 가장 양호하였다. 2. 자구의 형성은 엽조직 및 생장점조직을 2,4 -D 2.0mg/ +kinetin 0.2mg/ 또는 2.4-D 2.0g/ +kinetin 2.0mg/ 을 첨가한 배지에서 양호하였고 근 및 괴경조직에서는 자구가 전혀 ?성되지 않았다. 3. 엽의 부위별 자구형성능력은 Petiole이 자구형성수, 크기, 엽면적 및 생체중에 있어서 marginal meristem, minor vein area 및 intercostal area 보다 양호하였다. 4. 경의 부위별 자구형성능력은 상부위 조직일수록 자구의 형성수 크기, 엽면적 및 생체중이 양호하였으나 하부위일수록 불량하였다. 5. 배양온도는 25℃ 에서 callus 유기, 크기 및 생체중이 가장 양호하였다. 6. 배지의 pH는 4.5~7.5에서 모두 callus가 유기되었으나 그중 pH 6.0 배지에서 가장 양호하였다. 7. 재생되는 식물체 일부가 광엽화되는 경향이었으며 소수의 albino 식물체와 변이체도 관찰할 수 있었다. In order to find out the best media, explants and environmental conditions for induction of calluses and organogeneses of Pinellia ternata (Thunb.) Breit in vitro, various parts of adult have been cultured on Murashige & Skoog's medium containing various levels of 2,4-dichlorophenoxy acetic acid(2,4-D) and kinetin. The results obtained were as follows: Calluses were induced from the surface of apical meristem and leaf tissue. Formation and growth of calluses in petiole ex plants were best on the MS medium complemented with 2,4-D 2.0 mg/l and kinetin 0.2mg/l. But callus formation in stem ex plants of the nearest tuber was not induced at all kinds of media. Plantlets occured at all treatment except absence of growth regulator. Their numbers, size, leaf and fresh weight were promoted by 2,4-D 2.0mg/l and kinetin 0.2mg/l. Root growth was increased on the medium containing higher 2,4-D concentrations. Size and fresh weight of callus were increased at 25~circC compared with 10, 20 and 30~circC , respectively. Optimal pH value was at 6.0 for growth of callus. Morphological aberrations were observed in plantlets, especially in regenerated leaves. The separation of the broad leaved plantlets and albino were observed in some cultures. Growth of plantlets after transplantation was best in pots with the sterilized vermiculte. But abnormal variants withered up.

Usefulness of Percutaneous Puncture in Insertion of Totally Implantable Venous Access Devices in Pediatric Patients

최정식,박근명,정성택,홍기천,전용선,조순구,최윤미 대한혈관외과학회 2017 Vascular Specialist International Vol.33 No.3

Purpose: Totally implantable venous access devices (TIVADs) are commonly used in pediatrics for the administration of chemotherapy, antibiotics, or parenteral nutrition. TIVADs can be implanted using various techniques, including surgical cutdown (SC) and percutaneous puncture (PP). Recently, percutaneous TIVAD became popular in adults, but studies comparing between PP and SC group in pediatric patients are rare. Materials and Methods: Data were collected and analyzed retrospectively from 23 patients who underwent TIVAD at a single institution between January 2013 and December 2015. We examined the clinical characteristics, insertion techniques, and clinical outcome. We divided the patients into 2 groups and compared PP with ultrasonography and SC using the insertion technique. We compared success rate, procedural time, and the patency rate between the 2 groups. Results: Eleven TIVADS were inserted using PP, and 12 TIVADs were inserted using SC. No statistically significant difference in characteristics was found between the 2 groups. The procedural time in the PP group was shorter than that in the SC group, but the difference was not statistically significant (P=0.685). During followup, 1 patient in the SC group had an occlusion, and 1 patient in the PP group had an infection. Conclusion: PP of the internal jugular vein with ultrasonography appears to be the method of choice for TIVAD insertion owing to its similar success rate in terms of implantation and complication rate to that in SC, with shorter procedural times in pediatric patients.

[LAW & LIFE] 공동주택의 층간 소음분쟁

최정식 전력문화사 2015 Electric Power Vol.9 No.10

[LAW & LIFE] 불효자 방지법 제정 논란을 보면서

최정식 전력문화사 2015 Electric Power Vol.9 No.9

해외연수기 : 공무원의 채취가 잘 스며든 복지의 낙원 스위스

최정식 대한지방행정공제회 2000 地方行政 Vol.49 No.560

[LAW & LIFE] 한센병 환자에 대한 국가의 강제수술 위법성

최정식 전력문화사 2017 Electric Power Vol.11 No.4

[LAW & LIFE] 경업금지위반에 따른 영업행위금지 및 손해배상청구

최정식 전력문화사 2018 Electric Power Vol.12 No.3

선내 제조된 혼합연료 품질 개선을 위한 초음파 적용 가능성에 관한 연구

최정식,주해지,한원희 해양환경안전학회 2017 해양환경안전학회지 Vol.23 No.1

이 연구는 초음파 에너지의 공동현상(Cavitation)을 이용하여 선내 제조된 혼합연료유의 문제점을 개선하고 혼합연료유의 안정적인 사용이 가능하도록 하여 선박운용비의 상당부분을 차지하는 연료비를 절감하고자 한다. 실험은 선내 혼합연료유 제조 방식을 모사하여 선박용 연료유 M.G.O(Marine Gas Oil)와 MF-180(Marine Fuel-oil 180)를 각각 부피비 기준으로 0.25:0.75 및 0.75:0.25 비율로 혼합하였으며 초음파 처리장치를 이용하여 혼합연료유에 초음파 에너지를 직접 조사하여 초음파 에너지가 혼합연료유에 미치는 영향에 관해 고찰하였다. 실험결과, 선내 혼합유 제조시 보고되었던 문제점을 확인하였으며, 혼합시료유의 초음파 조사 후 잔류탄소량은 최대 28.4 % 감소하였다. 또한, 잔류탄소량 감소 및 분산 안정성 분석결과를 토대로 초음파 에너지에 의한 캐비티의 붕괴압이 연료입자 미립화에 효과가 있고, 중질연료유가 많이 함유된 혼합연료유의 일시적인 가용성을 높일 수 있을 것으로 판단되었다. In this study, contributes to improving the state of this problem using cavitation by ultrasonic energy to reduce fuel costs, which take up a considerable part of ship operation costs, by making the use of on-board blended fuel oil more stable. An experiment simulating on-board blending methods was completed. Fuel (M.G.O & MF-180) was mixed at a volume ratio of 0.25:0.75 and, 0.75:0.25, and the effect of ultrasonic energy on blended fuel oil was examined after applying ultrasonic energy to blended fuel oil using an ultrasonic treatment unit. With the results, we confirmed the blending problem reported by vessels and residual carbon was reduced by up to 28.4%. In addition, based on the results for reduction of residual carbon content and dispersion stability, it was confirmed that the collapse pressure of the cavity due to the ultrasonic energy was effective to atomization of fuel particle and the temporary availability of mixed fuel containing a heavy fuel increased.

어머니

崔正植 법조협회 1996 法曹 Vol.45 No.7

증권집단소송에서 부실표시의 중요성의 소송허가요건해당성에 관한 한국과 미국 법제의 비교연구 ― 대법원 2016.11.4. 선고 2015마4027 결정을 중심으로 ―

최정식 한국증권법학회 2018 증권법연구 Vol.19 No.1

It is a controversial case about whether the substantive content of a claim for damages should be heard at the stage of making a decision to permit a class action. According to the view that the class action should be granted only when the importance of misrepresentation is recognized, it is necessary for the defendant to examine the case of the preliminary hearing at the permission stage because the class action suffers a lot of losses from the disadvantage. In particular, a small number of US Supreme Court's view that trust is estimated by market fraud theory in securities fraud lawsuits, If the estimate is broken, it should not be a decision to grant a lawsuit permission. In addition, SECURITIES-RELATED CLASS ACTION ACT provides for the separation of the litigation permission procedure and the trial of the main litigation, and in the litigation permitting stage, a legal claim or a de facto important issue is applied to all the members as a claim for damages under Article 3 (1). The Supreme Court has the opinion that the importance of misrepresentation is not a litigation permission requirement, since it is sufficient to prove that it is common, and that the importance of misrepresentation is examined at the substantive stage of the case. Korea Supreme Court adopted the view of the majority opinion of the US Supreme Court. In the efficient securities market which is disclosed by accepting the market fraud theory, defendant's misrepresentation or omission is reflected in the stock price, It is assumed that the buyer bought the stock that the price is fair price, because the buyer relied on the misrepresentation or omission. However, at the stage of granting a securities class action, it is not necessary to prove the importance of the misstatement, but it is sufficient to prove the commonality of legal or real issues. Considering that it is not easy to approve a lawsuit in securities class action, it is reasonable for the Supreme Court to consider that the importance of misrepresentation should not be examined during the permission process. 집단소송 허가를 결정하는 단계에서 부실표시의 중요성을 심리하여야 하는지에 대하여 견해가 엇갈리고 있다. 미국 연방대법원의 다수의견은 부실표시의 중요성에 대한 입증은 시장사기이론에 기초한 증권사기사건의 집단소송허가단계에서 심리하여야 할 필수 요건이 아니며, 부실표시의 중요성은 압도성 요건을 판단하는데 필요한 집단구성원의 공통 쟁점이라고 한다. 그래서 부실표시의 중요성은 소송허가단계에서 심리할 필요가 없으며 사실심리의 단계에서 증명하면 된다고 하였다. 우리 증권집단소송법이 소송허가절차와 본안심리절차를 분리하고 있는바, 소송허가단계에서는 실체적 심리에 해당되는 중요성을 심리할 필요가 없으며, 다만 집단의 사실적 또는 법률적 쟁점이 공통된다는 점만 증명하면 된다고 대법원이 판시하고 있는데, 이는 미국 연방대법원은 다수 의견과 맥을 같이 하고 있다. 우리 대법원이 증권신고서 등의 허위기재로 인한 증권집단소송에서 허위기재의 중요성의 심리는 소송허가요건이 아니라 본안심리 사항이라고 판단한 것은 소송허가가 어렵도록 법이 규정하고 있다는 점을 고려할 때 타당하다고 생각된다.