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RISS 인기검색어
- 검색키워드
- 저자 : 孫奎元(Kyu Won Sohn) (검색결과 3 건)
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孫奎元 中央醫學社 1963 中央醫學 Vol.4 No.5
Tissue factors extracted from various normal organs which inhibit significantly the growth of animal cancers were reported by Chin and Kim (Report of 1961, " supported by the Damon-Runyon Fund; J. Korean Surgical Society, Vol. 3, No. 4,1961). In 1960, certain substance from animal bone marrow which inhibit cancer growth were reported too by Rosenstein. The following experiments were conducted to investigate further precisely the effect of tissue factors extracted from rabbit bone marrow on the transplanted cancers and normal tissues of mice. Materials and Methods 1) As animal cancers, Ehrlich ascites cancer and N-F sarcoma(Nakahara-Fukuoka) were used. As to the method of tumor transplantation, Hwang' s method was applied. Tissue factors were prepared from bone marrow of adult and young rabbits in the following manner. Homogenates of the bone marrow (I step substance) were centrifuged in a model PR1 international centrifuge at 15, 000/rpm. Ethyl alcohol was added to the supernatant and the sediment (Ⅱ step substance) was placed under the electrophoresis in sodium barbital and barbital buffer. The slow moving fraction was treated with trypsin (]1 step substance) and the material was dialized against distilled water in a cellophane tube. The material in the cellophane tube was removed and precipitated by acetone. The sediment was ready for injection as 10% solution (Ⅳ step substance). The injections were carried out after tumor transplantation in mice. A single dosis of 0.1 ml, 0.3 ml and 0.5 ml of the tissue factor was injected in individual animal groups once daily. On the 18th day after tumor transplantation, animals were sacrificed and tumors removed and weighed. The growth of the tumors were measured by average length of 2 diameters of the tumors and these were compared with those of control group. 2) Tissue factors were prepared, as mentioned above, from bone marrow and liver of young rabbits. Experiments were carried out on the normal mice. A single dosis of 0.3 ml of 10% of each tissue factors were injected intraperitoneally daily. In every 3 days, animals were sacrificed, the liver and bone marrow of the femur removed, and then fixed in Carnoy' s solution. After dehydration and decalcification, the tissues were embedded in paraffin, cut in sections 5 micron thick, and. stained with hematoxylin and eosin. Mitosis was counted in 100 fields on each slide with the help of 0.65 mm objective lens and 12.5x ocular. These counts were compared with those of the control group in which 0.3 ml of saline solution was, injected instead of tissue factor. Results 1) The toxic effect of this tissue factor was studied by administration in normal mice. As shown in Table 13, the toxic reaction was negligible normal animals in this experiments. As shown in Table 1, 2 and Figure 1, in the animals injected with bone marrow homogenate (I step substance), the growth of Ehrlich ascites cancer(solid form) was significantly promoted, and the degree of promotion being apparently more marked in the group injected with young bone marrow homogenate than in the group injected- with adult bone marrow homogenate. As the relation of doses, in animals injected with 0, 1 ml of tissue factor, the tumor growth was more markedly accelerated than in the groups injected with 0.3 ml and 0, 5ml of tissue factor. The degree of tumor growth promotion was directly proportional to tissue factor doses. In the animals injected with Ⅱ step substance, the growth was slightly inhibited (Table 3; 4. Fig. 2). The degree of inhibition was more. marked in the group injetced with young bone marrow extracts than in the animals injected with adult bone marrow factor. The degree of inhibition was 0.5 ml, 0.3 ml and 0.1 ml. In the animals injected with Ⅲ step substance, the growth was considerably inhibited(Table 5, 6. Fig. 3). The tendency of inhibition was the same as in the animals injected with Ⅱ step substance. As shown in Table 7-10, and Figure 4, in animis injected with Ⅳ step substance, the inhibition of growth was marked and regression was observed thereafter, but the mortality rate increased. The tumor removed on the 12th day showed a marked decrease in tumor mass weight. 2) Mitosis counts of bone marrow and liver after the intraperitoneal injection of 0.3 ml of 10% solution of tissue factor in mice once daily for 3 days, showed that tissue factors generally inhibited cellular mitosis (Table 11, 12. Fig. 5, 6). The liver factor markedly inhibited liver tissue and the bone marrow factor markedly' inhibited bone marrow tissue. Mitosis count, thereafter, showed that the tissue factors markedly promoted cellular mitosis. The liver factor markedly promoted that of liver tissue and the bone marrow factor markedly promoted that of bone marrow tissue. It was noted that mitosis count decreased significantly with the application of small amount of the tissue factor, but was increased with a great quantity of tissue factor. At the same time, it is speculated that the tissue factor has tissue specificity.
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