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RISS 인기검색어
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- 저자 : 呂邑東 (검색결과 2 건)
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野生담배(Nicotiana glauca) Callus의 生長과 葉綠素形成에 미치는 Pnytohormone, Sucrose 및 光度의 影響
呂邑東,蘇祥燮,金必榮 全北大學校 基礎科學硏究所 1989 基礎科學 Vol.12 No.1
To establish a basis of a photoautotrophic culture, effects of phytohormones, sucrose, and light intensity on growth and chlorophyll foundation of callus were investigated. The callus was induced from the leaf explants of a wild tobacco(Nicotiana glauca Graham) on the MS medium supplemented with 1.0 ㎎/ℓof 2,4-D. Growth of them was arrived at logistic phase for 16 days of culsure which exhibited growth rate of 187.5 mg per day, and arrived at early stationary phase for 28 days. Both NAA in auxins and kinetin in cytokinins known to be effective chlorophyll formation were used as phytohormone. Callus growth and chlorphyll formation were the most effective at the combination of 0.1 ㎎/ℓof NAA and 1.0 ㎎/ℓof kinetin. And chlorophyll a/b was increased according to the increase of the concentration of kinetin. Callus growth was enhanced according to the increase of the concentration of sucrose in the cases supplemented with 0.0, 0.5, 1.0, 2.0 % sucrose respectively to the above medium, but did not make a difference in the cases supplemented with 3.0% and 4.0% sucrose respectively to it. However, chlorophyll formation was not affected by the sucrose concentration in the medium but associated with growth rates of callus in media with various sucrose levels(r=0.89**). On the other hand, the increase in light intensity from 2,000 through 5,000 to 10,000ux stimulated callus growth and chlorophyll formation. Chlorophyll a/b was also enhanced according to the increase of light intensity. However, soluble protein contents of callus were decreased according to growth phase of callus.
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담배 綠色과 白色 Callus로부터 分離된 原形質體의 融合 및 培養
呂邑東,蘇雄永 全北大學校 基礎科學硏究所 1983 基礎科學 Vol.6 No.1
To investigate the colony formation activities of the fused protoplasts, the protoplasts isolated from green and white calli of tobacco (Nicotiana glauca) were fused by the PEG treatments and cultured in MS medium. The satisfactory yields (90%) of isolated protoplasts in white and green calli were taken 4 and 5 hours, respectively. The rates were depended upon the 40% PEG (MW,4,000) volumes added to 100 μ1 protoplats (about 4×10^5/ml). The most effective volume, 100 μ1 PEG exhibited the high fusion rate (37%). The fused protoplasts suspended in MS medium supplemented with 2,4-D 3mg/1, kinetin 0.5mg/1 and 0.4M glucose as osmoticum were cultured. Their first and second cell divisions were observed on the 2nd and 4th day, respectively. And their colonies were observed on the 7th day.
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