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Serap Duraklı-Velioğlu,İsmail Hakkı Boyacı,Osman Şimşek,Tuncay Gümüş 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.6
The combined effect of temperature, agitationspeed, and light on red pigment production by Monascuspurpureus (M. purpureus) Went DSM 1604 using bugdamaged wheat was studied using an artificial neuralnetwork (ANN). Information retrieved from the ANN wasused to determine the optimal operating conditions forpigment production by M. purpureus using bug damagedwheat meal. The developed ANN had R2 values fortraining, validation, and testing data sets of 0.993, 0.961,and 0.944, respectively. According to the model, thehighest pigment production of 1.874 absorbance units at510 nm (A510 nm) would be achieved at 29oC and 150 rpmunder light conditions. The mean value of the experimentalresults obtained under these optimum conditions was1.787±0.072 A510 nm, corresponding to a pigment yield of35.740 A510 nm/g. The study showed that bug damagedwheat can be used as a substrate for red pigment productionby M. purpureus.
Mineral content analysis of root canal dentin using laser-induced breakdown spectroscopy
Selen Küçükkaya Eren,Emel Uzunoğlu,Banu Sezer,Zeliha Yılmaz,İsmail Hakkı Boyacı 대한치과보존학회 2018 Restorative Dentistry & Endodontics Vol.43 No.1
Objectives This study aimed to introduce the use of laser-induced breakdown spectroscopy (LIBS) for evaluation of the mineral content of root canal dentin, and to assess whether a correlation exists between LIBS and scanning electron microscopy/energy dispersive spectroscopy (SEM/EDS) methods by comparing the effects of irrigation solutions on the mineral content change of root canal dentin. Materials and Methods Forty teeth with a single root canal were decoronated and longitudinally sectioned to expose the canals. The root halves were divided into 4 groups (n = 10) according to the solution applied: group NaOCl, 5.25% sodium hypochlorite (NaOCl) for 1 hour; group EDTA, 17% ethylenediaminetetraacetic acid (EDTA) for 2 minutes; group NaOCl+EDTA, 5.25% NaOCl for 1 hour and 17% EDTA for 2 minutes; a control group. Each root half belonging to the same root was evaluated for mineral content with either LIBS or SEM/EDS methods. The data were analyzed statistically. Results In groups NaOCl and NaOCl+EDTA, the calcium (Ca)/phosphorus (P) ratio decreased while the sodium (Na) level increased compared with the other groups (p < 0.05). The magnesium (Mg) level changes were not significant among the groups. A significant positive correlation was found between the results of LIBS and SEM/EDS analyses (r = 0.84, p < 0.001). Conclusions Treatment with NaOCl for 1 hour altered the mineral content of dentin, while EDTA application for 2 minutes had no effect on the elemental composition. The LIBS method proved to be reliable while providing data for the elemental composition of root canal dentin.