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여영근,최병국,임아영,김효정,김수민,김대곤 한국유가공기술과학회 1998 Journal of Dairy Science and Biotechnology (JMSB) Vol.16 No.2
The lipids of milk provide energy and many essential nutrients for the newborn animal. They also have distinctive physical properties that affect the processing of dairy products. Milk fat globules mainly consist of neutral lipids like triacylglycerols, whereas the globule membranes contain the complex lipids mostly. Phospholipids are a small but important fraction of the milk lipids and are found mainly in the milk fat globule membrane and other membranous material in the skim-milk phase. The milk fats of ruminant animals are characterized by the presence of relatively high concentrations of short-chain fatty acids, especially butyric and hexanoic acids, which are rarely found in milks of non-ruminants. The fatty acids of milk lipids arise from de novo synthesis in the mammary gland and uptake from the circulating blood. The fatty acid compositions of milks are usually complex and distinctive, depending on the nature of the fatty acids synthesized de novo in the mammary gland and those received from the diet in each species. The content and composition of milks from different species vary widely: presumably, these are evolutionary adaptations to differing environments. The actual process by which these globules are formed is unkonwn, but there are indications that triglyceride-containing vesicles which bleb from endoplasmic reticulum may serve as nucleation sites for globules. Recent studies on milk have centred on the manipulation of milk lipids to increase specific fatty acids, i.e. 20-carbon omega-3 fatty acids (eicosapentaenoic acid 20:5n3, decosahexaenoic acid 22:6n3) from marine sources because the fatty acids are closely associated with a decreased risk of coronary heart disease.
Shin, M.J.,Kim, D.W.,Jo, H.S.,Cho, S.B.,Park, J.H.,Lee, C.H.,Yeo, E.J.,Choi, Y.J.,Kim, J.A.,Hwang, J.S.,Sohn, E.J.,Jeong, J.H.,Kim, D.S.,Kwon, H.Y.,Cho, Y.J.,Lee, K.,Han, K.H.,Park, J.,Eum, W.S.,Choi, Pergamon ; Elsevier Science Ltd 2016 FREE RADICAL BIOLOGY AND MEDICINE Vol.97 No.-
Proline rich Akt substrate (PRAS40) is a component of mammalian target of rapamycin complex 1 (mTORC1) and is known to play an important role against reactive oxygen species-induced cell death. However, the precise function of PRAS40 in ischemia remains unclear. Thus, we investigated whether Tat-PRAS40, a cell-permeable fusion protein, has a protective function against oxidative stress-induced hippocampal neuronal (HT-22) cell death in an animal model of ischemia. We showed that Tat-PRAS40 transduced into HT-22 cells, and significantly protected against cell death by reducing the levels of H<SUB>2</SUB>O<SUB>2</SUB> and derived reactive species, and DNA fragmentation as well as via the regulation of Bcl-2, Bax, and caspase 3 expression levels in H<SUB>2</SUB>O<SUB>2</SUB> treated cells. Also, we showed that transduced Tat-PARS40 protein markedly increased phosphorylated RRAS40 expression levels and 14-3-3σ complex via the Akt signaling pathway. In an animal ischemia model, Tat-PRAS40 effectively transduced into the hippocampus in animal brain and significantly protected against neuronal cell death in the CA1 region. We showed that Tat-PRAS40 protein effectively transduced into hippocampal neuronal cells and markedly protected against neuronal cell damage. Therefore, we suggest that Tat-PRAS40 protein may be used as a therapeutic protein for ischemia and oxidative stress-induced brain disorders.
Measurement of the refractive index of the LAB-based liquid scintillator and acrylic at RENO
Yeo, I S,Jang, J H,Kim, M S,Ahn, J K,Baik, S R,Choi, E I,Choi, Seonho,Choi, Y,Jang, H I,Jang, J S,Jeon, E J,Jeong, I S,Joo, K K,Kim, B C,Kim, H S,Kim, J Y,Kim, S B,Kim, S H,Kim, W,Kim, Y D,Lee, J,Lim, Royal Swedish Academy of Sciences 2010 Physica scripta Vol.82 No.6
<P>In this paper, we describe the first measurement of the refractive index of the linear alkyl benzene (LAB)-based liquid scintillator and acrylic used at the Reactor Experiment for Neutrino Oscillation (RENO). The refractive index of a material is an important optical property and the prism spectrometer is the most common device for measuring the refractive index. Using the minimum deviation technique, which requires an exact measurement of the apex angle, we determined the refractive indices at six different wavelengths and then fitted them. We also compared the refractive index of the LAB-based liquid scintillator with the refractive indices of other liquid scintillator solvents commonly used in reactor neutrino experiments.</P>
여동준(D. J. Yeo),최명수(M.S. Choi),변정환(J. H. Byun),서정주(J. J. Suh),양정규(J. K. Yang),문덕홍(D. H. Moon) 한국동력기계공학회 2006 한국동력기계공학회 학술대회 논문집 Vol.- No.-
This paper deals with the free vibrations of folded plates by the transfer influence coefficient method. The governing equations of a non-circular cylindrical shell including a plate as special case are written in a coupled set of first order matrix differential equations using the transfer matrix. The Runge-Kutta-Gill Integration method are used to solve the governing differential equation. The natural frequencies and corresponding mode shapes are calculated numerically for the folded plate of various crank angles. And all boundary conditions and the intermediate supports between folded plate and foundation could be treated only by adequately varying the values of the spring constants.
여동준(D. J. Yeo),최명수(M. S. Choi),변정환(J. H. Byun),양정규(J. K. Yang),서정주(J. J. Suh) 한국동력기계공학회 2007 한국동력기계공학회 학술대회 논문집 Vol.- No.-
In this paper, the authors formulated analytical algorithm for the in-plane free vibration of a annular plate with intermediate support part by the transfer influence coefficient method (TICM) which is based on the successive transmission of influence coefficients on nodal circumferences. To confirm the effectiveness of the suggested algorithm, we analyzed the free vibration of a annular plate with three typical intermediate support parts by the TICM and the Transfer matrix method (TMM) on a personal computer. The computational result was that the TICM was superior to the TMM in the in-plane free vibration analysis of a annular plate with rigid intermediate supports.
Yeo, I.A.,Yoon, S.H.,Yee, J.J. Applied Science Publishers 2013 APPLIED ENERGY Vol.104 No.-
This study proposes a method to create an urban planning support model, applying the Environment and energy Geographical Information System Database (E-GIS DB) through the urban life cycle to reduce energy use for environmentally friendly urban planning. The results deduced from this study are organized as follows. The proposed E-GIS construction model is composed of (a) an urban GIS integration model, (b) an E-GIS DB model, and (c) a visualization model. The urban GIS integration model has the ability to integrate urban GIS constructs as well as to connect and visualize urban planning and the environment and energy DB in 3D space. The E-GIS DB model includes a function to visualize the 2D and 3D information, which is used in the environment and energy planning of a city. To validate the proposed E-GIS construction model, a Korean city undergoing the urban planning process is selected as a case study. An E-GIS DB section with an 8kmx12km area for the research subject area was constructed in 2D and 3D GIS, and the urban space, climate elements, and energy distribution characteristics are compared.
Yeo, J.S.,Seong, D.W.,Hwang, S.H. Korean Society of Industrial and Engineering Chemi 2015 Journal of industrial and engineering chemistry Vol.31 No.-
The pristine lignin was chemically modified by hydrolysis condensation reaction using (3-aminopropyl) triethoxysilane and then was subsequently treated with polypropylene-graft-maleic anhydride to give MAPP anchored lignin (MAPP-a-Lignin). The modified lignin particles prepared at each steps were characterized by FT-IR, SEM-EDX and XPS measurements. Polypropylene composites with MAPP-a-Lignin were prepared through melt-blending method. In this work, the effects of content of MAPP-a-Lignin on the properties of the polypropylene composites were investigated. From the results of mechanical properties, melting/crystallization behaviors and the morphologies of the composites, it was found that there was a stronger interfacial interaction between MAPP-a-Lignin and polypropylene in the composites.
Yeo, C.D.,Kim, J.W.,Kim, S.C.,Kim, Y.K.,Kim, K.H.,Kim, H.J.,Lee, S.,Rhee, C.K. Grune Stratton 2012 Journal of critical care Vol.27 No.6
Objective: Despite an improvement in the prognosis of patients with hematologic malignancies, the mortality of such patients transferred to the intensive care unit (ICU) is high. This study determined the predictors of mortality in a cohort of critically ill patients with hematologic malignancies admitted to the ICU. Methods: We studied 227 critically ill patients with hematologic malignancies who were admitted to the ICU between April 2009 and December 2011. A cohort of consecutive patients with hematologic malignancies was reviewed retrospectively to identify clinically useful prognostic factors. Results: The ICU mortality rate was 84.1%, and the in-hospital mortality rate was 89.9%. The ICU mortality was significantly higher in patients with acute leukemia than in those with other malignancies. A significant difference between survivors and nonsurvivors was found in neutropenia and its recovery during the ICU stay, presence of cardiac dysfunction, the need for an invasive mechanical ventilator, use of inotropic/vasopressor agents, platelet count, aspartate transaminase level, pH, and Acute Physiology And Chronic Health Evaluation II score. In the multivariate analysis, acute leukemia, need for invasive mechanical ventilator, use of inotropic/vasopressor agents, and Acute Physiology And Chronic Health Evaluation II scores were independently associated with a worse outcome in patients with hematologic malignancies admitted to the ICU. Conclusion: Higher mortality in patients with hematologic malignancies admitted to the ICU is associated with more severe illness, as reflected by higher organ failure scores or respiratory or hemodynamic instability. Mortality is higher in patients with acute leukemia as compared with other hematologic malignancies.
Characterization of the sensor domain of QseE histidine kinase from Escherichia coli
Yeo, K.J.,Park, J.W.,Kim, E.H.,Jeon, Y.H.,Hwang, K.Y.,Cheong, H.K. Academic Press 2016 Protein expression and purification Vol.126 No.-
In enterohemorrhagic Escherichia coli (EHEC), the QseEF two-component system causes attaching and effacing (AE) lesion on epithelial cells. QseE histidine kinase senses the host hormone epinephrine, sulfate, and phosphate; it also regulates QseF response regulator, which activates LEE gene that encodes AE lesion. In order to understand the recognition of ligand molecules and signal transfer mechanism in pathogenic bacteria, structural studies of the sensor domain of QseE of Escherichia coli should be conducted. In this study, we describe the overexpression, purification, and structural and biophysical properties of the sensor domain of QseE. The fusion protein had a 6xHis tag at its N-terminus; this protein was overexpressed as inclusion bodies in E. coli BL21 (DE3). The protein was denatured in 7M guanidine hydrochloride and refolded by dialysis. The purification of the refolded protein was carried out using Ni-NTA affinity column and size-exclusion chromatography. Thereafter, the characteristics of the refolded protein were determined from NMR, CD, and MALS spectroscopies. In a pH range of 7.4-5.0, the folded protein existed in a monomeric form with a predominantly helical structure. <SUP>1</SUP>H-<SUP>15</SUP>N HSQC NMR spectra shows that approximately 93% backbone amide peaks are detected at pH 5.0, suggesting that the number of backbone signals is sufficient for NMR studies. These data might provide an opportunity for structural and functional studies of the sensor domain of QseE.