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        A Methanol Wxtract of Adansonia digitata L. Leaves Inhibits Pro-Iflammatory iNos Possibly via the Inhibition of NF-kB Activation

        ( Yihunie Ayele ),( Jung Ah Kim ),( Eun Hee Park ),( Ye Jung Kim ),( Negussie Retta ),( Gulelat Dessie ),( Sang Ki Rhee ),( Kwangoh Koh ),( Kung Woo Nam ),( Hee Seon Kim ) 한국응용약물학회 2013 Biomolecules & Therapeutics(구 응용약물학회지) Vol.21 No.2

        This study examined the eoeal polyphenol content of eight wild edible from Ethiopia and their effect on NO production in Raw264. 7 cells. Owing to its relatively high polyphenol con and inhibition of NO production, the methanol extract of Ad-ansonia digitata L. leaf (MEAD) was subjected to detailed evaluation of its antioxidant and anti-inflammatoryeffcts. Antioxidant effects were assessed by measuring free-radical-scavenging activity using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and oxygen-radical-absorbance capacity (ORAC) assays, while anti-inflammatory effects were assessed by measuring inducible nitric oxide synthase (iNOS) expression in lipopolysaccharide (LPS)-stimulated RAW264. 7 cells. ln the ORAC assay, Mead was 10. 2 times more potent than vitamin C at eliminatinf peroxyl radicals. ln DPPH assay, MEAD inhibited lkBa degradation and NF-kB trans-location from the cytosol to the nucleus in LPS-inflammatory iNOS expression, which might be related to the elimination of peroxyl radicals amd thus the inhibition of lkBa-mediated NF-kB signal transduction.

      • Removal of methylene blue from textile waste water using kaolin and zeolite-x synthesized from Ethiopian kaolin

        Zemedkun Mulushewa,Wendimagegn Tagesse Dinbore,Yihunie Ayele 환경독성보건학회 2021 환경독성보건학회지 Vol.36 No.1

        The wastewater generated from textile factories is linked to one of the main water pollution problems; therefore, it is important to reduce the pollutants in industrial effluents before their discharge into environment. The present study was to investigate the appropriateness zeolite-x and kaolin as effective adsorbents for removal of methylene blue from the textile wastewater. Batch adsorption experiments were carried out to assess parameters that influence the adsorption process. The prepared zeolite-x and kaolin were characterized by Fourier Transform Infrared and X-ray diffraction techniques. The results of this study showed that the particle size is 40.77 nm and 0.45 nm kaolin and zeolite-x respectively. The performance of zeolite-x adsorbent is best at the optimum pH 4 with removal efficiency of 97.77% and kaolin adsorbent at pH 6 with removal efficiency of 86.86%. The optimum contact time was obtained at 60 and 80 minutes for zeolite–x and kaolin respectively. While optimum adsorbent dosage was obtained at 0.4 and 0.6 grams with removal efficiency of 97.12% and 87.75% for the zeolite-x and kaolin adsorption experiment respectively. The confirmed square sum errors values are 1.0×10<SUP>-4</SUP> and 1.0×10<SUP>-3</SUP> for zeolite-x and kaolin, respectively. The Adsorption isotherms results have well fitted to Freundlich isotherm than Langmuir isotherm. The adsorption kinetics results were best fitted the pseudo second order model. The result shows that the zeolite-x has high removal efficiency than kaolin at the same operating conditions. Application of this method can be economically, environmentally, and socially feasible to address wastewater problems. Further research has to be carried out on the removal capacity of this adsorbent for organic dyes not only from the textile industry but also from leather industries and soap industries.

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