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- 저자 : ( Yanni Han ) (검색결과 4 건)
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Effects of sheep slaughter age on myogenic characteristics in skeletal muscle satellite cells
Han Yunfei,Guo Wenrui,Su Rina,Zhang Yanni,Yang Le,Borjigin Gerelt,Duan Yan 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.4
Objective: The objective of this study was to investigate the effects of sheep slaughter age on myogenic characteristics in skeletal muscle satellite cells (SMSCs).Methods: Primary SMSCs were isolated from hind leg biceps femoris muscles of Wurank lambs (slaughtered at three months, Mth-3) and adults (slaughtered at fifteen months, Mth-15). SMSCs were selected by morphological observation and fluorescence staining. Myogenic regulatory factors (MRF) and myosin heavy chain (MyHC) expressions of SMSCs were analyzed on days 1, 3, 4, and 5.Results: The expressions of myogenic factor 5 (Myf5), myogenic differentiation (MyoD), Myf6, and myogenin (MyoG) in Mth-15 were significantly higher in Mth-15 than in Mth-3 on days 1, 3, and 4 (p<0.05). However, MyoG expression in Mth-15 was significantly lower than in Mth-3 on day 5 (p<0.05). The expressions of MyHC I, MyHC IIa, and MyHC IIx in Mth-15 were significantly higher than in Mth-3 on days 1 and 3 (p<0.05), and MyHC IIb were significantly lower than in Mth-3 on days 3 and 4 (p<0.05). In contrast, the expression of MyHC IIx in Mth-15 was significantly lower and MyHC IIb was significantly higher than in Mth-3 on days 5 (p<0.05).Conclusion: The slaughter age altered the expression of MRFs and MyHCs in SMSCs while differentiation, which caused the variation of myogenic characteristics, and thus may affect the meat quality of Wurank sheep. Objective: The objective of this study was to investigate the effects of sheep slaughter age on myogenic characteristics in skeletal muscle satellite cells (SMSCs). Methods: Primary SMSCs were isolated from hind leg biceps femoris muscles of Wurank lambs (slaughtered at three months, Mth-3) and adults (slaughtered at fifteen months, Mth-15). SMSCs were selected by morphological observation and fluorescence staining. Myogenic regulatory factors (MRF) and myosin heavy chain (MyHC) expressions of SMSCs were analyzed on days 1, 3, 4, and 5. Results: The expressions of myogenic factor 5 (Myf5), myogenic differentiation (MyoD), Myf6, and myogenin (MyoG) in Mth-15 were significantly higher in Mth-15 than in Mth-3 on days 1, 3, and 4 (p<0.05). However, MyoG expression in Mth-15 was significantly lower than in Mth-3 on day 5 (p<0.05). The expressions of MyHC I, MyHC IIa, and MyHC IIx in Mth-15 were significantly higher than in Mth-3 on days 1 and 3 (p<0.05), and MyHC IIb were significantly lower than in Mth-3 on days 3 and 4 (p<0.05). In contrast, the expression of MyHC IIx in Mth-15 was significantly lower and MyHC IIb was significantly higher than in Mth-3 on days 5 (p<0.05). Conclusion: The slaughter age altered the expression of MRFs and MyHCs in SMSCs while differentiation, which caused the variation of myogenic characteristics, and thus may affect the meat quality of Wurank sheep.
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The effect of constitutive spins on finite inelastic strain simulations
Cho, Han Wook,Dafalias, Yannis F. Techno-Press 1997 Structural Engineering and Mechanics, An Int'l Jou Vol.5 No.6
Within the framework of anisotropic combined viscoplastic hardening formulation, accounting macroscopically for residual stress as well as texture development at finite deformations of metals, simple shear analyses for the simulation of fixed-end torsion experiments for ${\alpha}$-Fe, Al and Cu at different strain rates are reviewed with an emphasis on the role of constitutive spins. Complicated responses of the axial stresses with monotonically increasing shear deformations can be successfully described by the capacity of orthotropic hardening part, featuring tensile axial stresses either smooth or oscillatory. Temperature effect on the responses of axial stresses for Cu is investigated in relation to the distortion and orientation of yield surface. The flexibility of this combined hardening model in the simulation of finite inelastic strains is discussed with reference to the variations of constitutive spins depending upon strain rates and temperatures.
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An Energy Harvesting Aware Routing Algorithm for Hierarchical Clustering Wireless Sensor Networks
( Chaowei Tang ),( Qian Tan ),( Yanni Han ),( Wei An ),( Haibo Li ),( Hui Tang ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.2
Recently, energy harvesting technology has been integrated into wireless sensor networks to ameliorate the nodes` energy limitation problem. In theory, the wireless sensor node equipped with an energy harvesting module can work permanently until hardware failures happen. However, due to the change of power supply, the traditional hierarchical network routing protocol can not be effectively adopted in energy harvesting wireless sensor networks. In this paper, we improve the Low-Energy Adaptive Clustering Hierarchy (LEACH) protocol to make it suitable for the energy harvesting wireless sensor networks. Specifically, the cluster heads are selected according to the estimation of nodes` harvested energy and consumed energy. Preference is given to the nodes with high harvested energy while taking the energy consumption rate into account. The utilization of harvested energy is mathematically formulated as a max-min optimization problem which maximizes the minimum energy conservation of each node. We have proved that maximizing the minimum energy conservation is an NP-hard problem theoretically. Thus, a polynomial time algorithm has been proposed to derive the near-optimal performance. Extensive simulation results show that our proposed routing scheme outperforms previous works in terms of energy conservation and balanced distribution.
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Ge Tian,Li Xiong,Wenhua Lin,Jinghao Han,Xiang-Yan Chen,Thomas Wai Hong Leung,Yannie Oi Yan Soo,Lawrence Ka Sing Wong 대한신경과학회 2016 Journal of Clinical Neurology Vol.12 No.3
Background and PurposezzExternal counterpulsation (ECP) is a noninvasive method used to enhance cerebral perfusion by elevating the blood pressure in ischemic stroke. However, the response of the beat-to-beat blood pressure variability (BPV) in ischemic stroke patients during ECP remains unknown. MethodszzWe enrolled recent ischemic stroke patients and healthy controls. Changes in the blood fow velocities in bilateral middle cerebral arteries and the continuous beat-to-beat blood pressure before, during, and afer ECP were monitored. Power spectral analysis revealed that the BPV included oscillations at very low frequency (VLF; <0.04 Hz), low frequency (LF; 0.04– 0.15 Hz), and high frequency (HF; 0.15–0.40 Hz), and the total power spectral density (TP; <0.40 Hz) and LF/HF ratio were calculated. ResultszzWe found that ECP signifcantly increased the systolic and diastolic blood pressures in both stroke patients and controls. ECP decreased markedly the systolic and diastolic BPVs at VLF and LF and the TP, and the diastolic BPV at HF when compared with baseline. Te decreases in diastolic and systolic BPV reached 37.56% and 23.20%, respectively, at VLF, 21.15% and 12.19% at LF, 8.76% and 16.59% at HF, and 31.92% and 23.62% for the total TP in stroke patients, which did not difer from those in healthy controls. Te change in fow velocity on the contralateral side was positively correlated with the total TP systolic BPV change induced by ECP (r=0.312, p=0.035). ConclusionszzECP reduces the beat-to-beat BPV when increasing the blood pressure and cerebral blood fow velocity in ischemic stroke patients. ECP might be able to improve the clinical outcome by decreasing the beat-to-beat BPV in stroke patients, and this should be explored further in future studies.
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