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( Min Wu Jie Tang ),( Xuerui Zhou ),( Dan Lei ),( Chaoyi Zeng ),( Hong Ye Ting Cai ),( Qing Zhang ) 한국미생물 · 생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.2
Continued fenvalerate use has caused serious environmental pollution and requires large-scale remediation. Dibutyl phthalate (DBP) was discovered in fenvalerate metabolites degraded by Citrobacter freundii CD-9. Coculturing is an effective method for bioremediation, but few studies have analyzed the degradation pathways and potential mechanisms of cocultures. Here, a DBPdegrading strain (BDBP 071) was isolated from soil contaminated with pyrethroid pesticides (PPs) and identified as Stenotrophomonas acidaminiphila. The optimum conditions for DBP degradation were determined by response surface methodology (RSM) analysis to be 30.9 mg/l DBP concentration, pH 7.5, at a culture temperature of 37.2℃. Under the optimized conditions, approximately 88% of DBP was degraded within 48 h and five metabolites were detected. Coculturing C. freundii CD-9 and S. acidaminiphila BDBP 071 promoted fenvalerate degradation. When CD-9 was cultured for 16 h before adding BDBP 071, the strain inoculation ratio was 5:5 (v/v), fenvalerate concentration was 75.0 mg/l, fenvalerate was degraded to 84.37 ± 1.25%, and DBP level was reduced by 5.21 mg/l. In addition, 12 fenvalerate metabolites were identified and a pathway for fenvalerate degradation by the cocultured strains was proposed. These results provide theoretical data for further exploration of the mechanisms used by this coculture system to degrade fenvalerate and DBP, and also offer a promising method for effective bioremediation of PPs and their related metabolites in polluted environments.
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The establishment of IB-SEM numerical method and verification of fluid-solid interaction
Wang, Jing,Li, Shu-cai,Mao, Xuerui,Li, Li-ping,Shi, Shao-shuai,Zhou, Zong-qing Techno-Press 2018 Geomechanics & engineering Vol.15 No.6
The interaction between particles and fluid was investigated by IB-SEM numerical method which is a combination of combing the spectral/hp element method and the rigid immersed boundary method. The accuracy of this numerical method was verified based on the computed results with the traditional body-fitted mesh in numerical simulation of the flow through the cylinder. Then the governing equations of particles motion and contact in fluid are constructed. The movement of the particles and the interaction between the fluid and the particles are investigated. This method avoided the problem of low computational efficiency and error caused by the re-division of the grid when the solids moved. Finally, the movement simulation of multi particles in the fluid was carried out, which can provide a completely new numerical simulation method.
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