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Sivaprasad, V.,Nataraju, B.,Renu, S.,Datta, R.K. Korean Society of Sericultural Science 2003 International Journal of Industrial Entomology Vol.6 No.1
Infectious flacherie of silkworm Bombyx mori is caused by B. mori infectious flacherie virus (BmIFV) and causes severe crop loss to sericulturists. In the present study, a colloidal textile dye-based dipstick immunoassay is developed for the detection of infectious flacherie in silkworms. Colloidal textile dye (blue D2R) with Aλ$_{max}$ at 620 nm was sensitised with 500 $\mu\textrm{g}$/ml of purified anti-BmIFV IgG. The dye-antibody reagent detects purified antigen up to 10 ng/ml and BmIFV infection in diseased larval extracts $(up to a dilution of {10^-5})$ and faecal matter extracts $(up to a dilution of {10^-2})$ by forming clear blue dot within 30 min. It was observed to be stable for three months period at $4^{\circ}C$. The efficacy of textile dye-based dipstick immunoassay was on pay with HRP-based dipstick immunoassay and fluorescent antibody test, and better than latex agglutination and ouchterlony tests in the detection of BmIFV The dye-based dipstick immunoassay method provides a simple, sensitive and less expensive test for the detection of BmIFV infection in silkworms.s.
$F(ab)_2$-ELISA for the Detection of Nuclear Polyhedrosis Virus of Silk-worm, Bombyx mori L.
Sivaprasad, V.,Nataraju, B.,Baig, M.,Samson, M.V.,Datta, R.K. Korean Society of Sericultural Science 2003 International Journal of Industrial Entomology Vol.6 No.2
$F(ab`)_2$-ELISA and direct antigen coating-ELISA (DAC-ELISA) were evaluated in the detection of purified Bombyx mori nuclear polyhedrosis virus (BmNPV) and nuclear polyhedrosis virus infection in silkworm larvae inoculated with BmNPV polyhedra. Although nanogram levels of BmNPV was detected in both DAC- and $F(ab`)_2$-ELISA, similar concentrations of antigen was detected in case of F(ab’)$_2$-ELISA even at higher dilution of antibody (up to 1 : 20 K). One hundred percent nuclear polyhedrosis infection was detected 6 hrs after inoculation in BmNPV infected silkworm larvae by $F(ab`)_2$-ELISA. On the other hand, detection of 100% infection was observed only three days after inoculation in DAC-ELISA. In this study, it was observed $F(ab`)_2$-ELISA was more sensitive than DAC-ELISA in the detection of purified BmNPV as well as nuclear polyhedrosis infection in silkworm larvae.
JANOWSKI STARLIKENESS AND CONVEXITY
KANIKA KHATTER,V. Ravichandran,S. SIVAPRASAD KUMAR 장전수학회 2017 Proceedings of the Jangjeon mathematical society Vol.20 No.4
Certain necessary and sufficient conditions are determined for the functions f(z) = z - P1 n=2 anzn, an ≥ 0, defined on the open unit disk, to belong to various subclasses of starlike and convex func- tions. Also discussed are certain sufficient conditions for the normalised analytic functions f of the form (z=f(z))μ = 1 + P1 n=1 bnzn, μ ∈ C to belong to the class of Janowski starlike functions.
KUMAR, S. SIVAPRASAD,TANEJA, H. C.,RAVICHANDRAN, V. 대한수학회 2006 Kyungpook mathematical journal Vol.46 No.1
In this paper, the authors introduce new classes of p-valent functions defined by Dziok-Srivastava linear operator and the multiplier transformation and study their properties by using certain first order differential subordination and superordination. Also certain inclusion relations are established and an integral transform is discussed.
Kumar, S. Sivaprasad,Taneja, H.C.,Ravichandran, V. Department of Mathematics 2006 Kyungpook mathematical journal Vol.46 No.1
In this paper, the authors introduce new classes of p-valent functions defined by Dziok-Srivastava linear operator and the multiplier transformation and study their properties by using certain first order differential subordination and superordination. Also certain inclusion relations are established and an integral transform is discussed.