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( G. Lokesh ),( P. K. Kar ),( A. K. Srivastava ),( Saloni Swaroopa ),( M. K. Sinha ) 한국잠사학회 2012 International Journal of Industrial Entomology Vol.24 No.2
Tropical tasar silkworm Antheraea mylitta Drury experiences extreme temperature stress conditions during its life cycle particularly during diapauses and first crop. The present study witnessed the impact of high temperature on some biochemical profiles and egg production (fecundity) of semi-domesticated Daba and Shorea robusta (Sal) based wild ecorave Laria during seed cocoon (pupa) preservation. Cocoons of Daba and Laria were treated with high temperature at 40℃ for 10 days in a Bod incubator. The protein profile and carbohydrate content in the hemolymph and fat body and total haemocyte count (THC) in the hemolymph of pupa were investigated. Further, the fecundity and fertility of egg was assessed. Significant increase in the protein concentration was observed in the hemolymph with reduction in the fat body (p<0.05). The difference in protein concentration was highly significant between the semi-domesticated Daba and wild ecorace Laria (p<0.05). High pupal mortality(20%) and reduced fecundity(10-15%) in Daba was noticed compared to wild Laria. Also an increased THC(>2800) was recorded in Laria. The study infers the potentials of wild ecoraces in sustaining the extreme temperature conditions and need of adopting suitable package of practices for the preservation of diapause seed cocoons during extreme summer conditions. There is possibility to introgression thermal stress resistant traits in the semi-domesticated races of tasar.
Lokesh, G.,Kar, P.K.,Srivastava, A.K.,Swaroopa, Saloni,Sinha, M.K. Korean Society of Sericultural Science 2012 International Journal of Industrial Entomology Vol.24 No.2
Tropical tasar silkworm Antheraea mylitta Drury experiences extreme temperature stress conditions during its life cycle particularly during diapauses and first crop. The present study witnessed the impact of high temperature on some biochemical profiles and egg production (fecundity) of semi-domesticated Daba and Shorea robusta (Sal) based wild ecorace Laria during seed cocoon (pupa) preservation. Cocoons of Daba and Laria were treated with high temperature at $40^{\circ}C$ for 10 days in a BOD incubator. The protein profile and carbohydrate content in the hemolymph and fat body and total haemocyte count (THC) in the hemolymph of pupa were investigated. Further, the fecundity and fertility of egg was assessed. Significant increase in the protein concentration was observed in the hemolymph with reduction in the fat body (p<0.05). The difference in protein concentration was highly significant between the semidomesticated Daba and wild ecorace Laria (p<0.05). High pupal mortality (20%) and reduced fecundity (10-15%) in Daba was noticed compared to wild Laria. Also an increased THC (>28000) was recorded in Laria. The study infers the potentials of wild ecoraces in sustaining the extreme temperature conditions and need of adopting suitable package of practices for the preservation of diapause seed cocoons during extreme summer conditions. There is possibility to introgression thermal stress resistant traits in the semi-domesticated races of tasar silkworm by resorting to conventional breeding plans with wild races and keeping the thermal stress induced response as markers.
K. Vijayan,C. V. Nair,P. K. Kar,T. P. Mohandas,B. Saratchandra,S. Raje Urs 한국잠사학회 2005 International Journal of Industrial Entomology Vol.10 No.1
Genetic diversity within and between populations of Antheraea mylitta Drury was studied using thirty individuals from three ecoraces using 12 ISSR and 10 RAPD primers. Raily, Daba and Modal ecoraces were collected from Chattisgarh, Jharkhand and Orissa states of India respectively. The ISSR and RAPD primers generated 94.7% and 95.6% polymorphism among the 30 individuals. The cluster analysis grouped these individuals according to their ecorace. The intra-ecoracial heterozygosity estimated with ISSR markers were 0.123 ± 0.18, 0.169 ± 0.17 and 0.214 ± 0.17 respectively for Modal, Raily and Daba ecoraces. Like wise, with RAPD markers the intraecoracial heterozygosity was 0.17 ± 0.22 in Modal, 0.229 ± 0.17 in Raily and 0.23 ± 0.19 in Daba ecoraces.However, the significantly low genetic differentiation (GST) (0.182 for ISSR and 0.161 for RAPD) and the high gene flow (Nm) (2.249 for ISSR and 2.60 for RAPD markers) among the ecoraces revealed that the amount of genetic diversity present among the ecoraces is not significant enough to make drastic genetic drifts among these ecoraces in the near future.
Jena K.,Kumari Ruchi,Pandey J.P.,Kar P.K.,Akhtar J.,Singh A.K.,Gupta V.P.,A. K. Sinha 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3
Sericin were isolated and characterized from tasar cocoons raised in three different food plants i.e. Terminalia arjuna, T. tomentosa and Shorea robusta for its applications. Their molecular composition, structure and physical nature were determined by elemental analysis, amino acid analysis, Fourier Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC) analysis. These results show that tasar food plants influence the physical and chemical properties of sericin. Further, sericin isolated from cocoons of S. robusta food plant shows better antioxidant potential and inhibition of tyrosinase, elastase and glutathione-S-transferase activity than other food plants. This may be attributed to its amino acid variations and associated phenolic content. The present study appears to be useful in utilizing tasar sericin as a potential bio-molecule for its prospective utili zation in pharmaceuticals and its associated fields.
Vijayan K.,Nair C. V.,Kar P. K.,Mohandas T. P.,Saratchandra B.,Urs S. Raje Korean Society of Sericultural Science 2005 International Journal of Industrial Entomology Vol.10 No.1
Genetic diversity within and between populations of Antheraea mylitta Drury was studied using thirty individuals from three ecoraces using 12 ISSR and 10 RAPD primers. Rally, Daba and Modal ecoraces were collected from Chattisgarh, Jharkhand and Orissa states of India respectively. The ISSR and RAPD primers generated $94.7\%$ and $95.6\%$ polymorphism among the 30 individuals. The cluster analysis grouped these individuals according to their ecorace. The intra-ecoracial heterozygosity estimated with ISSR markers were $0.123{\pm}0.18,\;0.169{\pm}0.17\;and\;0.214{\pm}0.17$ respectively for Modal, Raily and Daba ecoraces. Like wise, with RAPD markers the intraecoracial heterozygosity was $0.17{\pm}0.22$ in Modal, $0.229{\pm}0.17$ in Raily and $0.23{\pm}0.19$ in Daba ecoraces. However, the significantly low genetic differentiation (GST) (0.182 for ISSR and 0.161 for RAPD) and the high gene flow (Nm) (2.249 for ISSR and 2.60 for RAPD markers) among the ecoraces revealed that the amount of genetic diversity present among the ecoraces is not significant enough to make drastic genetic drifts among these ecoraces in the near future.
Saini, K.K.,Sharma, S.D.,Chanderkant,Kar, M.,Singh, D.,Sharma, C.P. North-Holland 2007 Journal of non-crystalline solids Vol.353 No.24
Nanocrystalline thin films of titanium dioxide have been fabricated on glass and silica substrates from partially hydrolyzed precursor solution. These films were subjected to heat treatment for 1h at temperatures 100, 200, 300, 400, 500, 600, 700, 800 and 900<SUP>o</SUP>C and characterized by XRD, SEM, XPS and optical techniques. As deposited films are found to be amorphous and also contain hydroxyl and organic functional groups. Films heat treated above 100<SUP>o</SUP>C do not contain hydroxyl and organic functional groups. Microcrystalline behavior is observed in the films heat treated above 300<SUP>o</SUP>C. Crystallite size increases from ∼5 to 50nm as sintering temperature is increased from 300 to 700<SUP>o</SUP>C. Formation of anatase phase with c-axis length 7.03A is observed in the films annealed up to 700<SUP>o</SUP>C. These films peel off from the substrate beyond 700<SUP>o</SUP>C annealing temperature. Density as well as refractive index of the films increases with increase in annealing temperature up to 700<SUP>o</SUP>C. Refractive index is found to show Cauchys behavior. Transmission better than 70% is observed in the visible range. There is a strong absorption around 370nm, which is attributed to band gap absorption of the material.
( T. P. Mohandas ),( K. Vijayan ),( P. K. Kar ),( A. K. Awasthi ),( B. Saratchandra ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.8 No.2
Genetic diversity within the natural populations of Daba ecorace of Antheraea mylitta Drury was studied using individual silkworms collected from the South Singhbhum district of Jharkhand state of India with 21 inter simple sequence repeat (ISSR) primers. A total of 148 bands were produced, of which 79% was polymorphic. The pair wise genetic distance among the individuals varied from 0.186 to 0.329. The dendrogram grouped the individuals into 3 major clusters. Nei`s heterozygosity analysis revealed 0.265±0.18 variability within the population. The high genetic variability present within the natural population of Daba ecorace of A. mylitta is indicative of their adaptational strategy in nature and have much importance for in situ conservation as well as utilization in breeding programs.
T. P. Mohandas,K. Vijayan,P. K. Kar,A. K. Awasthi,B. Saratchandra 한국잠사학회 2004 International Journal of Industrial Entomology Vol.8 No.2
Genetic diversity within the natural populations of Daba ecorace of Antheraea mylitta Drury was studied using individual silkworms collected from the South Singhbhum district of Jharkhand state of India with 21 inter simple sequence repeat (ISSR) primers. A total of 148 bands were produced, of which 79% was polymorphic. The pair wise genetic distance among the individuals varied from 0.186 to 0.329. The dendrogram grouped the individuals into 3 major clusters. Nei’s heterozygosity analysis revealed 0.265 ± 0.18 variability within the population. The high genetic variability present within the natural population of Daba ecorace of A. mylitta is indicative of their adaptational strategy in nature and have much importance for in situ conservation as well as utilization in breeding programs.