Personalized Skin Care Solution from Facial Analysis to Treatment

( Jeong-gun Lee ),( Shin Hee Cho ),( Bo Young Shin ),( Minseok S. Kim ),( Jung-hwan Park ) 한국피부장벽학회 2017 한국피부장벽학회지 Vol.19 No.1

Global home beauty market is booming at an average annual rate of 17% over US 4.1 Billion dollars. Because Steady and professional skin care is very important to maintain good skin condition. S-Skin`s goal is to prepare for the smart beauty era by combining traditional beauty technologies with IT technologies. S-Skin`s solution consists of 3 components. First, S-Skin beauty device that can measure skin condition and LED therapy automatically, General beauty devices on the market have only measurement function or care function, no both, Whereas S-Skin beauty device is an all-in-one type. Our beauty device is based on acquiring accurate skin information from skin measurement and providing skin care based on it. Second, micro needle patches that deliver the active ingredient deep into the skin, Also by using the patch material that changes color with body temperature makes it easy to visually check whether or not it is attached correctly. Further, with the smartphone, when tagging the NFC sticker on the patch or product package. the tagged date is displayed in the app`s calendar to easily manage the patches` frequency of usage. Finally, it consists of an app that recommends skin care method. It then recommends a suitable microneedle patch and LED light care for better skin. Microneedle patches, portable skin measurement and treatment device and smartphone application all working together to create professional-quality skin care solutions.

Microfabricated embryonic stem cell divider for large-scale propagation of human embryonic stem cells

Kim, Minseok S.,Kim, Janghwan,Han, Hyo-Won,Cho, Yee Sook,Han, Yong-Mahn,Park, Je-Kyun Royal Society of Chemistry 2007 Lab on a chip Vol.7 No.4

<P>We have developed a novel method for fabricating an embryonic stem cell divider (ESCD) constructed from a poly(dimethylsiloxane) (PDMS) replica with a square or hexagonal pattern, and have proposed a new dissociation method for human embryonic stem cells (ESCs). An aspect ratio of the device as high as 2 was perfectly replicated in the cutting line. Using the ESCD, human ESC colonies can be easily and efficiently dissociated into regular-sized ESC clumps without enzymatic treatment. The regularity of the ESC clumps dissociated by the ESCD was compared to that dissociated by a conventional mechanical method. Its quality and reliability were confirmed by maintaining undifferentiated ESCs up to the 15th passage. The ESCD will contribute to the advance quality control of <I>in vitro</I> ESC cultures and allow large-scale production of qualified ESCs with tremendous time- and work-saving.</P> <P>Graphic Abstract</P><P>This paper describes a novel method for fabricating an embryonic stem cell divider (ESCD) constructed from a poly(dimethylsiloxane) replica with a square or hexagonal pattern capable of dissociating human embryonic stem cell (ESC) colonies into regular-sized ESC clumps. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b617760n'> </P>

A microfluidic in vitro cultivation system for mechanical stimulation of bovine embryos

Kim, Minseok S.,Bae, Chae Yun,Wee, Gabbine,Han, Yong-Mahn,Park, Je-Kyun WILEY-VCH Verlag 2009 Electrophoresis Vol.30 No.18

<P>This work demonstrates a novel microfluidic in vitro cultivation system for embryos that improves their development using a partially constricted channel that mimics peristaltic muscle contraction. Conventional photolithography and a PDMS replica molding process were used to make straight or constricted microchannels. To investigate the effects of constriction geometry on embryonic development, different constriction widths of the channel were designed. Bovine embryos were loaded and incubated by simply placing them on a tilting machine to provide embryo movement via gravity. The fertilized embryos were cultivated on the microfluidic in vitro cultivation system until the blastocyst, hatching, or hatched blastocyst stages. To confirm the quality of blastocysts in the microfluidic channel, double staining was performed and compared with bovine embryos cultivated by the conventional droplet method. The proportion of eight-cell development among total embryos in the constricted channel (56.7±13.7%; mean±SD) was superior to that in the straight channel (23.9±11.0%). This suggests that the effect of constriction is vital for the early development of bovine embryos in assisted-reproduction research.</P>

A Microfluidic In Vitro Cultivation System for Bovine Embryos

Minseok S. Kim,Gabbine Wee,Chaeyoon Bae,Young-Mahn Han,Je-Kyun Park 한국생물공학회 2007 한국생물공학회 학술대회 Vol.2007 No.10

A Biomimetic Cell Culture Platform for Microfluidic Cell-based Assays

Minseok S. Kim(김민석),Ju Hun Yeon(연주헌),Je-Kyun Park(박제균) 한국생물공학회 2005 한국생물공학회 학술대회 Vol.2005 No.10

Functional fusion proteins and prevention of electrode fouling for a sensitive electrochemical immunosensor

Kim, A-Ram,Park, Tae Jung,Kim, Minseok S.,Kim, In-Ho,Kim, Ki-Suk,Chung, Kwang Hoe,Ko, Sungho Elsevier 2017 Analytica chimica acta Vol.967 No.-

<P><B>Abstract</B></P> <P>A highly sensitive electrochemical immunosensor was developed by preventing electrode fouling and using a novel fusion protein of silica binding polypeptides (SBP)-protein G (ProG) created by recombinant DNA technology as a functional crosslinker for rapid and self-oriented immobilization of antibodies onto silica nanoparticles (SiNPs). Antibody immobilization onto the SiNPs by the SBP-ProG could rapidly be achieved without any chemical treatment. The immunosensor was fabricated through bonding of a partially gold-deposited cyclic olefin copolymer (COC) (top substrate) and gold patterned interdigitated array COC electrode (bottom substrate). To prevent electrode fouling, human immunoglobulin G (hIgG) was immobilized onto the ceiling inside the microchannel, instead of the bottom electrode. Alkaline phosphatase (AP)-labeled anti-hIgG was allowed to immunoreact with hIgG on the ceiling, followed by addition of an enzyme to generate an oxidative peak current. A three-fold increase in current was observed from the immunosensor without any electrode fouling compared with a control with the protein functionalized electrode. Also, the SiNPs facilely coated with AP-anti-hIgG via the SBP-ProG could increase the electrochemical signal up to 20% larger than that of the AP-anti-hIgG alone. Furthermore, this immunosensor was ultrasensitive with a detection limit of 0.68 pg/mL of a biomarker associated with prostate cancer.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel fusion protein was developed as crosslinker for rapid self-oriented immobilization of antibody on SiNPs. </LI> <LI> This crosslinker contributed to easy formation of SiNPs/Ab complexes, resulting in electrochemical signal enhancement. </LI> <LI> Novel prevention method of electrode fouling was developed to enhance sensitivity of the electrochemical immunosensor. </LI> <LI> This immunosensor exhibited excellent sensitivity with the simple fabrication methods. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

합성곱 신경망을 이용한 실시간 상추 생육 센싱 연구

강민석 ( Minseok Gang ),김학진 ( Hak-jin Kim ),김동욱 ( Dong-wook Kim ) 한국농업기계학회 2021 한국농업기계학회 학술발표논문집 Vol.26 No.2

원예 작물의 생육 지표로 사용되는 생체중, 건물중, 높이, 엽면적, 직경은 증산 및 광합성과 밀접한 관계가 있고, 이러한 생육 지표는 작물 생산성을 정량화하여 최적의 환경, 양분, 관수 제어 전략을 수립하는데 활용될 수 있다. 따라서 정확한 생육 지표 측정은 매우 중요하지만, 생육 지표를 측정하기 위한 전통적인 방법은 파괴적이고 인적 자원을 많이 필요로 하여 시간과 비용 소모가 클 뿐만 아니라, 시료의 채취 방법이나 시료 상태에 따라 신뢰성이 떨어질 수 있다. 생육 지표 측정에는 정확성 뿐만 아니라 작물 성장 단계별 양액의 변량 공급을 위해 실시간으로 작물의 생육 정도를 측정하는 기술 또한 필요하다. 최근 딥러닝 기술의 발전으로 3차원 영상 데이터를 이용한 합성곱 신경망과 같은 기술이 도입되고 있어 생육 센싱을 비롯한 분야에서 농업 적용성이 높아지고 있다. 본 연구에서는 RGB 채널과 깊이 정보를 이용하여 4개 품종 상추의 생체중, 건물중, 높이, 엽면적, 직경을 예측할 수 있는 합성곱 신경망 기반 모델을 개발하고, 예측 성능을 구명하였다. 사용된 재료는 2021년 6월 바게닝긴대학교 주최 온라인 인공지능 경진대회에서 제공한 상추 데이터이며, 해당 데이터는 인텔 Realsense D435 카메라를 이용하여 수집된 상추의 RGB와 깊이 정보이다. 선행 연구에서 개발한 자동 3D 영상 데이터 수집 시스템 및 모니터링 시스템을 개선하여 합성곱 신경망 모델 적용이 가능하도록 하였으며, 측정 항목별 합성곱 신경망 예측 모델의 측정 성능을 분석한 결과 예측 모델은 결정계수 0.9 이상의 정확도를 나타냈으며 모니터링 시스템에서 이미지 하나당 평균 150 ms의 처리 속도를 나타내어 실시간 생육 지표 예측 가능성을 보였다.

Voxel-Based Dosimetry of Iron Oxide Nanoparticle-Conjugated ¹⁷⁷Lu-Labeled Folic Acid Using SPECT/CT Imaging of Mice

Gupta, Arun,Shin, Jae H.,Lee, Min S.,Park, Ji Y.,Kim, Kyuwan,Kim, Joong H.,Suh, Minseok,Park, Cho R.,Kim, Young J.,Song, Myung G.,Jeong, Jae M.,Lee, Dong S.,Lee, Yun-Sang,Lee, Jae S. American Chemical Society 2019 Molecular pharmaceutics Vol.16 No.4

<P>Several radiolabeled folic acid conjugates have been developed for targeted imaging and therapy. However, the therapeutic concept with radiolabeled folate conjugates has not yet been applied to clinical applications owing to the high renal absorbed dose. The effectiveness of targeted radionuclide therapy (TRT) depends primarily on the absorbed dose rate and on the total absorbed dose delivered to the tumor and to normal tissue. Owing to various limitations associated with organ level dosimetry, voxel-based dosimetry has become essential for the assessment of a more accurate absorbed dose during TRT. In this study, we synthesized iron oxide nanoparticle (IONP)-conjugated radiolabeled folate (<SUP>177</SUP>Lu-IONP-Folate) and performed voxel-based dosimetry using SPECT/CT images of normal mice through direct Geant4 application for emission tomography (GATE) Monte Carlo (MC) simulation. We also prepared <SUP>177</SUP>Lu-Folate and <SUP>177</SUP>Lu-IONPs for the comparison of absorbed doses with that of <SUP>177</SUP>Lu-IONP-Folate. In addition, we calculated the mean absorbed dose at the organ-level using the medical internal radiation dose (MIRD) schema. The radioactivities of all three radiotracers were mainly accumulated in the liver and kidneys immediately after injection. For the kidneys, the voxel-based absorbed doses obtained with <SUP>177</SUP>Lu-IONP-Folate, <SUP>177</SUP>Lu-Folate, and <SUP>177</SUP>Lu-IONPs were 1.01 ± 0.17, 2.46 ± 0.50, and 0.52 ± 0.08 Gy/MBq, respectively. The renal absorbed dose decreased significantly (∼half) when <SUP>177</SUP>Lu-IONP-Folate was used compared with when the <SUP>177</SUP>Lu-Folate only was used. The mean absorbed dose values obtained at organ-level using the MIRD schema were comparable to voxel-based absorbed doses estimated with GATE MC. The voxel-based absorbed dose values obtained in this study of individualized activity show that the renal absorbed dose could be reduced to almost half with <SUP>177</SUP>Lu-IONP-Folate. Therefore, <SUP>177</SUP>Lu-IONP-Folate could be clinically applicable in the TRT of folate receptor-positive cancers in a personalized manner when using the voxel-based dosimetry method.</P> [FIG OMISSION]</BR>

Solenoid Driven Pressure Valve System: Toward Versatile Fluidic Control in Paper Microfluidics

Kim, Taehoon H.,Hahn, Young Ki,Lee, Jungmin,van Noort, Danny,Kim, Minseok S. American Chemical Society 2018 ANALYTICAL CHEMISTRY - Vol.90 No.4

<P>As paper-based diagnostics has become predominantly driven by more advanced microfluidic technology, many of the research efforts are still focused on developing reliable and versatile fluidic control devices, apart from improving sensitivity and reproducibility. In this work, we introduce a novel and robust paper fluidic control system enabling versatile fluidic control. The system comprises a linear push–pull solenoid and an Arduino Uno microcontroller. The precisely controlled pressure exerted on the paper stops the flow. We first determined the stroke distance of the solenoid to obtain a constant pressure while examining the fluidic time delay as a function of the pressure. Results showed that strips of grade 1 chromatography paper had superior reproducibility in fluid transport. Next, we characterized the reproducibility of the fluidic velocity which depends on the type and grade of paper used. As such, we were able to control the flow velocity on the paper and also achieve a complete stop of flow with a pressure over 2.0 MPa. Notably, after the actuation of the pressure driven valve (PDV), the previously pressed area regained its original flow properties. This means that, even on a previously pressed area, multiple valve operations can be successfully conducted. To the best of our knowledge, this is the first demonstration of an active and repetitive valve operation in paper microfluidics. As a proof of concept, we have chosen to perform a multistep detection system in the form of an enzyme-linked immunosorbent assay with mouse IgG as the target analyte.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2018/ancham.2018.90.issue-4/acs.analchem.7b03791/production/images/medium/ac-2017-03791a_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac7b03791'>ACS Electronic Supporting Info</A></P>

A quantum dot-based microfluidic multi-window platform for quantifying the biomarkers of breast cancer cells

Kwon, Seyong,Kim, Minseok S.,Lee, Eun Sook,Sohn, Jang Sihn,Park, Je-Kyun Oxford University Press 2014 Integrative biology Vol.6 No.4

Conventional molecular profiling methods using immunochemical assays have limits in terms of multiplexity and the quantification of biomarkers in investigation of cancer cells. In this paper, we demonstrate a quantum dot (QD)-based microfluidic multiple biomarker quantification (QD-MMBQ) method that enables labeling of more than eight proteins immunochemically on cell blocks within 1 h, in a quantitative manner. An internal reference, beta-actin, was used as a loading control to compensate for differences in not only the cell number but also in staining quality among specimens. Furthermore, the microfluidic blocking method exhibited less nonspecific binding of QDs than the conventional static blocking method.