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Saravanan, Manoharan,Nam, Sang-Eun,Eom, Hye-Jin,Lee, Do-Hee,Rhee, Jae-Sung Elsevier 2019 COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLO Vol.216 No.-
<P><B>Abstract</B></P> <P>Low concentrations of nonylphenol (NP) in aquatic environment can induce drastic effects on the endocrine system in animals. In this study, we examined the modulatory effects of NP on reproductive and physiological parameters in juveniles of the red seabream and black rockfish following waterborne NP exposure (0, 1, 10, and 50 μg L<SUP>−1</SUP>) for 60 days. In red seabream exposed to 50 μg L<SUP>−1</SUP> NP, plasma levels of 17β-estradiol (E2) and 11-ketotestosterone (11-KT) were significantly lower at 30 and 60 days, while E2 levels were slightly higher in 10 μg L<SUP>−1</SUP>-exposed individuals at day 30. Similarly, significantly lower levels of E2 and 11-KT were observed in 10 and 50 μg L<SUP>−1</SUP>-exposed black rockfish at 60 days, whereas the E2 level was higher in 1 μg L<SUP>−1</SUP>-exposed individuals at day 30. After exposure to NP, plasma and mRNA levels of vitellogenin (VTG) were significantly higher in both species at 30 and 60 days, similar to the inducible effects from synthetic estrogen. Plasma cortisol levels were significantly elevated by relatively higher concentrations of NP (10 and 50 μg L<SUP>−1</SUP>) at 30 and 60 days. Finally, 60 days of exposure of 50 μg L<SUP>−1</SUP> NP significantly decreased the gonadosomatic index (GSI) and increased the hepatosomatic index (HSI) in both species. The results obtained from this study provide an evidence of the endocrine disrupting potential of waterborne NP on early stages of economically important marine fish. The NP-triggered endocrine modulation can induce effects on the development of reproductive and metabolic organs in fish species.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Direct evidence on accumulation of waterborne NP in marine fish blood. </LI> <LI> Strong endocrine disruption potential of NP on juvenile stage of marine fish. </LI> <LI> Analysis of NP-triggered stress induction and modulation of somatic parameters. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Manoharan Saravanan,김지윤,김해나,김성범,고동훈,허장현 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.4
Extensive use of fungicides in agriculture has led to the detrimental effects on aquatic ecosystems and organisms even at very low concentrations. In this respect, the present study was aimed to investigate the impact of environmentally relevant concentrations of fungicide isoprothiolane (2.7 and 27 lg/L; reported concentration in Yung San River, Korea) on a freshwater fish Cyprinus carpio for a short-term period of 96 h using various biomarkers such as hematological, ionoregulatory, biochemical, and enzymological parameters. At both concentrations, hemoglobin, hematocrit, red blood cell, serum glucose, cholesterol, triglycerides, aspartate aminotransferase, and gill Na?/K?-ATPase levels were significantly (p\0.05) decreased in fish treated with isoprothiolane. Contrastingly, white blood cell, serum sodium (Na?), chloride (Cl-), and albumin levels were increased (p\0.05). However, the short-term exposure of isoprothiolane in carp resulted in slight alterations in mean cellular volume, mean cellular hemoglobin, mean cellular hemoglobin concentration, serum potassium (K?), protein, globulin, and alkaline phosphatase levels. The present study concludes that alterations of hematological, ionoregulatory, biochemical, and enzymological parameters can be used as efficient biomarkers in monitoring the toxicity of isoprothiolane as well as other pesticides in aquatic organisms. Further, more detailed studies on using of specific biomarkers to monitor the long-term effects of isoprothiolane are needed.
Saravanan, Manoharan,Kim, Ji-Yoon,Kim, Hea-Na,Kim, Seong-Beom,Ko, Dong-Hoon,Hur, Jang-Hyun 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.4
Extensive use of fungicides in agriculture has led to the detrimental effects on aquatic ecosystems and organisms even at very low concentrations. In this respect, the present study was aimed to investigate the impact of environmentally relevant concentrations of fungicide isoprothiolane (2.7 and $27{\mu}g/L$; reported concentration in Yung San River, Korea) on a freshwater fish Cyprinus carpio for a short-term period of 96 h using various biomarkers such as hematological, ionoregulatory, biochemical, and enzymological parameters. At both concentrations, hemoglobin, hematocrit, red blood cell, serum glucose, cholesterol, triglycerides, aspartate aminotransferase, and gill $Na^+/K^+$-ATPase levels were significantly (p<0.05) decreased in fish treated with isoprothiolane. Contrastingly, white blood cell, serum sodium ($Na^+$), chloride ($Cl^-$), and albumin levels were increased (p\<0.05). However, the short-term exposure of isoprothiolane in carp resulted in slight alterations in mean cellular volume, mean cellular hemoglobin, mean cellular hemoglobin concentration, serum potassium ($K^+$), protein, globulin, and alkaline phosphatase levels. The present study concludes that alterations of hematological, ionoregulatory, biochemical, and enzymological parameters can be used as efficient biomarkers in monitoring the toxicity of isoprothiolane as well as other pesticides in aquatic organisms. Further, more detailed studies on using of specific biomarkers to monitor the long-term effects of isoprothiolane are needed.
Joe, Manoharan Melvin,Saravanan, Venkatakrishnan Sivaraj,Sa, Tongmin Springer-Verlag 2013 Archives of microbiology Vol.195 No.3
<P>The bacterial cell surface plays a major role in the bacterial aggregation that in turn plays a positive role in affecting the bacterial dispersion and survival in soil and their ability to adhere to plant surfaces. Plant growth-promoting Methylobacterium strains, Methylobacterium goesingense CBMB5, Methylobacterium sp. CBMB12, Methylobacterium oryzae CBMB20, Methylobacterium fujisawaense CBMB37, M. oryzae CBMB110 and Methylobacterium suomiense CBMB120 were evaluated for aggregation efficiency. Aggregation occurred in all test strains under high C/N growth conditions, and the strain CBMB12 showed the highest aggregation of 53.4?% at 72?h. Disaggregation compound treatment studies revealed the role of protein-protein interaction in Methylobacterium strains except CBMB110 and CBMB120 strains, where a possible carbohydrate-protein interaction is suspected. Surface layer protein extraction by LiCl followed by SDS-PAGE analysis showed the presence of proteins at molecular weights ranging from 41 to 49?kDa. Methylobacterium strains under aggregated conditions showed increased hydrophobicity compared to the cells under standard grown conditions. A relatively higher hydrophobicity of 50.1?% as evident by the adhesion with xylene was observed with strain CBMB12 under aggregated condition. This study reports the aggregation ability in plant growth-promoting Methylobacterium strains and the possible involvement of cellular components and hydrophobicity in this phenomenon.</P>
Parthiban Subramanian,Manoharan Melvin Joe,임우종,홍보희,Sherlyn C. Tipayno,Venkatakrishnan Sivaraj Saravanan,유재홍,정종배,Tahera Sultana,사동민 한국토양비료학회 2011 한국토양비료학회지 Vol.44 No.4
Cold-adapted bacteria survive in extremely cold temperature conditions and exhibit various mechanisms of adaptation to sustain their regular metabolic functions. These adaptations include several physiological and metabolic changes that assist growth in a myriad of ways. Successfully sensing of the drop in temperature in these bacteria is followed by responses which include changes in the outer cell membrane to changes in the central nucleoid of the cell. Their survival is facilitated through many ways such as synthesis of cryoprotectants,cold acclimation proteins, cold shock proteins, RNA degradosomes, Antifreeze proteins and ice nucleators. Agricultural productivity in cereals and legumes under low temperature is influenced by several cold adopted bacteria including Pseudomonas, Acinetobacter, Burkholderia, Exiguobacterium, Pantoea, Rahnella,Rhodococcus and Serratia. They use plant growth promotion mechanisms including production of IAA,HCN, and ACC deaminase, phosphate solublization and biocontrol against plant pathogens such as Alternaria, Fusarium, Sclerotium, Rhizoctonia and Pythium.