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Characterization of Two Urease-Producing and Calcifying Bacillus spp. Isolated from Cement
( Achal Varenyam ),( Abhijit Mukherjee ),( M. Sudhakara Reddy ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.11
Two bacterial strains designated as CT2 and CT5 were isolated from highly alkaline cement samples using the enrichment culture technique. On the basis of various physiological tests and 16S rRNA sequence analysis, the bacteria were identified as Bacillus species. The urease production was 575.87 U/ml and 670.71 U/ml for CT2 and CT5, respectively. Calcite constituted 27.6% and 31% of the total weight of sand samples plugged by CT2 and CT5, respectively. Scanning electron micrography analysis revealed the direct involvement of these isolates in calcite precipitation. This is the first report of the isolation and identification of Bacillus species from cement. Based on the ability of these bacteria to tolerate the extreme environment of cement, they have potential to be used in remediating the cracks and fissures in various building or concrete structures.
( Dhami Navdeep Kaur ),( M Sudhakara Reddy ),( Abhijit Mukherjee ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.5
Microbially induced calcium carbonate precipitation (MICCP) is a naturally occurring biological process that has various applications in remediation and restoration of a range of building materials. In the present investigation, five ureolytic bacterial isolates capable of inducing calcium carbonate precipitation were isolated from calcareous soils on the basis of production of urease, carbonic anhydrase, extrapolymeric substances, and biofilm. Bacterial isolates were identified as Bacillus megaterium, B. cereus, B. thuringiensis, B. subtilis, and Lysinibacillus fusiformis based on 16S rRNA analysis. The calcium carbonate polymorphs produced by various bacterial isolates were analyzed by scanning electron microscopy, confocal laser scanning microscopy, X ray diffraction, and Fourier transmission infra red spectroscopy. A strainspecific precipitation of calcium carbonate forms was observed from different bacterial isolates. Based on the type of polymorph precipitated, the technology of MICCP can be applied for remediation of various building materials.
Taxol Production by an Endophytic Fungus, Fusarium redolens, Isolated from Himalayan Yew
( Sanjog Garyali ),( Anil Kumar ),( M Sudhakara Reddy ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.10
Different endophytic fungi isolated from Himalayan Yew plants were tested for their ability to produce taxol. The BAPT gene (C-13 phenylpropanoid side chain-CoA acetyl transferase) involved in the taxol biosynthetic pathway was used as a molecular marker to screen taxolproducing endophytic fungi. Taxol extracted from fungal strain TBPJ-B was identified by HPLC and MS analysis. Strain TBPJ-B was identified as Fusarium redolens based on the morphology and internal transcribed spacer region of nrDNA analysis. HPLC quantification of fungal taxol showed that F. redolens was capable of producing 66 μg/l of taxol in fermentation broth. The antitumour activity of the fungal taxol was tested by potato disc tumor induction assay using Agrobacterium tumefaciens as the tumor induction agent. The present study results showed that PCR amplification of genes involved in taxol biosynthesis is an efficient and reliable method for prescreening taxol-producing fungi. We are reporting for the first time the production of taxol by F. redolens from Taxus baccata L. subsp. wallichiana (Zucc.) Pilger. This study offers important information and a new source for the production of the important anticancer drug taxol by endophytic fungus fermentation.
Enhancement of Taxol Production from Endophytic Fungus Fusarium redolens
Sanjog Garyali,Anil Kumar,M. Sudhakara Reddy 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.5
The optimization of taxol production by Fusariumredolens by one factor at a time (OFAT) approach led toproduction of 70 μg/L of taxol. With sucrose and NH4NO3as the carbon and nitrogen sources and medium volume(Vm) to flask volume (Vf) ratio of 0.2, a greater taxol productionwas attained. NH4NO3, MgSO4·7H2O and NaOAc at 6.25,0.63, and 1.25 g/L, were the significant factors for attainingthe highest taxol production. The optimization of culturevariables led to the production of taxol from 66 to 198 μg/L,which is three fold higher than that in the unoptimizedmedium. Current study results suggested the success ofResponse Surface Methodology in enhancing the productionof fungal taxol.