[PG-0007] Identification and Determination of Phenolic compounds in Rapeseed (Brassica napus L.) Mutant Lines Derived from Gamma-ray

Jaihyunk Ryu(Jaihyunk Ryu ),Baul Yang(Baul Yang ),Ye-Jin Lee(Ye-Jin Lee ),Dong-Gun Kim(Dong-Gun Kim ),Sang Hoon Kim(Sang Hoon Kim ),Sang-Il Han(Sang-Il Han ),Bo-Keun Ha(Bo-Keun Ha ),Si-Yong Kang(Si-Yo 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Genetic diversity and variation analysis of mutant lines derived from γ-ray and chemical mutagen treatments in blackberry (<i>Rubus fruticosus</i>)

Ryu, Jaihyunk,Ha, Bo-Keun,Kim, Dong Sub,Kim, Jin-Baek,Kim, Sang Hoon,Ahn, Joon-Woo,Jeong, Il Yun,Jo, Han-Jik,Kim, Ee-Yup,Kang, Si-Yong Cambridge University Press 2014 Plant genetic resources Vol.12 No.1

<P>Blackberry is a fruiting berry species with very high nutrient contents. With the recent increasing consumer demand for blackberries, new sources of germplasm and breeding techniques are required to improve blackberry production. This study was carried out to evaluate the genetic diversity (GD) and relationship among 55 blackberry (<I>Rubus fruticosus</I>) mutants derived from γ-ray treatment (52 lines) and <I>N</I>-methyl-<I>N</I>′-nitrosourea (MNU) treatment (three lines) using an inter-simple sequence repeat marker. A total of 18 bands were amplified with an average of 3.6 bands per primer. Among them, eight bands were identified to be polymorphic with a rate of 44.4%. In addition, the GD information content values were highest in the 60 Gy treatment population and the GD values were higher in the γ-ray treatment populations than in the MNU treatment population. According to a cluster analysis, all the mutant lines can be classified into five categories, and the genetic distance was greatest between the 80 Gy-irradiated population and other populations. These results indicate that mutant lines have high GD and can be effectively utilized for improving blackberry breeding.</P>

Effect of Gamiojeoksan Remnants used as Fertilizer on Growth and Yield of Korean mint

Jaihyunk Ryu,Seongkyu Choi,Kyeong Won Yun,Youngnam Seo,Kyoungsun Seo,Hyunjin Kim 한국자원식물학회 2009 한국자원식물학회지 Vol.22 No.3

In these experiments Gamiojeoksan remnant components were analyzed to recycle this medicinal herb remnant fertilizers. The basic growth of Korean mint by application of Gamiojeoksan remnants were higher than control. As the amount of fertilizers were increased, plant height, stem diameter, number of leaves and number of branches were increased. The growth and yield were the highest in the treatment of 30 g/pot. Weight of whole plant of Korean mint has a tendency to be heavy in application of herbs remnant than that of control.

Fruit Quality and Chemical Contents of Hybrid Boysenberry (Rubus ursinus) Lines Developed by Hybridization and Gamma Irradiation

( Jaihyunk Ryu ),( Soon-jae Kwon ),( Yeong Deuk Jo ),( Hong-il Choi ),( Kyung-yun Kang ),( Bo Mi Nam ),( Dong-gun Kim ),( Chang-hyun Jin ),( Jin-baek Kim ),( Ee-yup Kim ),( Seung Cheol Oh ),( Bo-keun 한국육종학회 2017 Plant Breeding and Biotechnology Vol.5 No.3

The Rubus fruit is an economically important berry crop that contains various functional compounds. The objective of this study was to analyze fruit qualities (i.e., pH, soluble solids content, titratable acidity, and mineral content) as well as fatty acid and phenolic compounds (i.e., ellagic acid and anthocyanins) among hybrid boysenberry lines developed by hybridization and gamma irradiation. There were no significant differences in the hybrid boysenberry fruit pH and titratable acidity (%) among the tested genotypes. However, the soluble solids content was higher in the BSA119 and BSA144 mutants than in the original genotype (BS_Hybrid). Meanwhile, linoleic acid was the most abundant fatty acid in the analyzed hybrid boysenberry fruits. The fatty acid composition did not differ significantly among the genotypes. The ellagic acid content of all genotypes ranged from 8.72 mg/100 g to 46.10 mg/100 g, with the highest concentration observed for the BSB127 genotype. Additionally, cyanidin-3-O-sophoroside (M-H⁺, 611 m/z) and cyanidin-3-O-glucoside (M-H⁺, 449 m/z) were the two major anthocyanins detected in the boysenberry and mutant genotypes, while cyanidin-3-O-glucoside was the predominant blackberry anthocyanin. The total anthocyanin concentrations of four mutant genotypes (i.e., BSA036, BSA078, BSA101, and BSB127) were significantly higher than that of the original genotype (382.0 mg/100 g). The highest total anthocyanin concentration was observed for the BSA078 genotype (467.9 mg/100 g). These results may be useful for identifying the optimal genotypes for breeding new cultivars with enhanced qualities and potential health benefits.

Phytochemicals and antioxidant activity in the kenaf plant (Hibiscus cannabinus L.)

Ryu, Jaihyunk,Kwon, Soon-Jae,Ahn, Joon-Woo,Jo, Yeong Deuk,Kim, Sang Hoon,Jeong, Sang Wook,Lee, Min Kyu,Kim, Jin-Baek,Kang, Si-Yong The Korean Society of Plant Biotechnology 2017 식물생명공학회지 Vol.26 No.4

Chemical compounds from four different tissues of the kenaf plant (Hibiscus cannabinus), a valuable medicinal crop originating from Africa, were examined to determine its potential for use as a new drug material. Leaves, bark, flowers, and seeds were harvested to identify phytochemical compounds and measure antioxidant activities. Gas chromatography mass spectrometry analyses identified 22 different phytocompounds in hexane extracts of the different parts of the kenaf plant. The most abundant volatile compounds were E-phytol (32.4%), linolenic acid (47.3%), trisiloxane-1,1,1,5,5,5-hexamethyl-3,3-bis[(trimethylsilyl)oxy] (16.4%), and linoleic acid (46.4%) in leaves, bark, flowers, and seeds, respectively. Ultra-high performance liquid chromatography identified the major compounds in the different parts of the kenaf plant as kaemperitrin, caffeic acid, myricetin glycoside, and p-hydroxybenzoic acid in leaves, bark, flowers, and seeds, respectively. Water extracts of flowers, leaves, and seeds exhibited the greatest DPPH radical scavenging activity and SOD activity. Our analyses suggest that water is the optimal solvent, as it extracted the greatest quantity of functional compounds with the highest levels of antioxidant activity. These results provide valuable information for the development of environmentally friendly natural products for the pharmaceutical industry.

Assessment of Growth and Seed Oil Composition of Kenaf (Hibiscus cannabinus L.) Germplasm

Jaihyunk Ryu,강시용,하보근,김동섭,김진백,Sang Hoon Kim 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.4

This study was conducted to evaluate the growth characteristics and fatty acid composition among 15 kenaf mutants derived fromthe kenaf germplasm C14 and 15 kenaf accessions originating from Russia, India, China, Iran, and Italy. The overall growth performance(plant height, stem diameter, flowering date, leaf, and flower size) of the stem color mutant lines derived from C14 are similarto those of the original variety. However, the flower color mutant lines derived from C14 showed flowering to occur 10 days laterwhen compared with the original variety and showed smaller leaf sizes than the original variety. Late-ripened kenaf accessions(Jinju, Auxu, and Jnagdae) can yield more bio-mass compared with early or medium-maturing germplasm. The late maturity kenaf(Auxu, Jinju, and Jangdae) has a higher oil percentage than the early maturity germplasm. Linoleic, oleic, and palmitic acids were thepredominant fatty acids in all kenaf seeds. The stem color mutant lines significantly surpassed the parental means of all saturatedfatty acids. In addition, the flower color mutant lines showed broad ranges of variation in oleic acid. The 15 accessions showed awide range of fatty acid compositions, spanning from 29.75 to 38.30% saturated fatty acids and 61.70 to 70.24% total unsaturatedfatty acids, and the late maturity kenaf has a higher linoleic acid percentage than the early maturity germplasm. The flowering periodwas highly positively (P ² 0.01) correlated with the plant height, stem diameter, oil percent, and linolenic acid (C18:3), and it was significantlynegatively (P ² 0.01) correlated with stearic acid (C18:0). These results will provide valuable information to assist theparental selection of kenaf breeding.

Variation Analysis of in vitro Cultured Plant Rhizomes Based on RAPD

Jaihyunk Ryu,Chang-Hyu Bae 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04

RAPD (random amplified polymorphic DNA) analysis was examined to detect variation of in vitro cultured 30 rhizomes of Cymbidium goeringii and Cymbidium kanran, with long-term subculture, respectively. Out of 105 DNA bands detected, the 38 were polymorphic with a polymorphic rate 36.1% in the C. goeringii. Out of 126 DNA bands detected, the 58 were polymorphic with a polymorphic rate 46.0% in the C. kanran. The size of the amplified fragments of the two species ranged from about 200bp to 3,000bp. Genetic similarity matrix (GSM) shows from 0.810 to 1.00 with an average of 0.929 in the rhizomes of C. goeringii and 0.786 to 0.984 with an average of 0.915 in the C. kanran. The result indicate that the long-term in vitro cultured C. goeringii and C. kanran supplemented with growth regulators might be promoted in higher polymorphism and increased GSM. Thus, the higher polymorphism rate of in vitro cultured rhizomes might be resulted in long-term subculture and the plant growth regulators supplemented with the culture medium. The results provide as fundamental data to develop a new materials for plant breeding and resources plant.

Single nucleotide polymorphism (SNP) discovery through genotyping-by-sequencing (GBS) and genetic characterization of <i>Dendrobium</i> mutants and cultivars

Ryu, Jaihyunk,Kim, Woon Ji,Im, Juhyun,Kang, Kyung-Won,Kim, Sang Hoon,Jo, Yeong Deuk,Kang, Si-Yong,Lee, Jeong-Hee,Ha, Bo-Keun Elsevier 2019 Scientia horticulturae Vol.244 No.-

<P><B>Abstract</B></P> <P> <I>Dendrobium</I> plants are the most important orchids used in horticultural industries and oriental medicine. Recently, several <I>Dendrobium</I> mutant genotypes with improved horticultural traits have been developed using various mutagens such as gamma irradiation, somaclonal variation, and aerospace mutagenesis. This study investigated genetic diversity and variations among 7 <I>Dendrobium</I> mutant genotypes and 11 commercial <I>Dendrobium</I> cultivars using single nucleotide polymorphism (SNP) markers generated from genotyping-by-sequencing (GBS) analysis. In addition, the SNPs were converted into a competitive allele-specific PCR (KASP) assay to discriminate the <I>Dendrobium</I> genotypes. A total of 67,669,394 clean data reads were generated, with 3.7 million reads per genotype on average, and 38,168,915 (56.4%) mapped reads. A total of 517,660 SNPs were detected, which included 443,305 homozygous and 74,355 heterozygous loci. The frequency of transition substitutions was shown to be higher than that of transversions, and the transition:transversion ratio is 1.34. A set of 37,721 filtered SNPs was used to perform a phylogenetic analysis, which showed that there were clear differences among the <I>Dendrobium</I> genotypes based on their species. A total of 18 SNPs were used for the development of KASP assay sets, of which 10 were successfully applied to distinguish among <I>Dendrobium</I> genotypes. This study demonstrated that the SNPs and KASP assay sets are an economically efficient tool for mutant screening and for selection of elite genotypes in <I>Dendrobium</I> breeding programs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> This study investigated genetic diversity in <I>Dendobrium</I> genotypes and developed KASP marker sets using SNPs generated from GBS analysis. </LI> <LI> A total of 517,660 SNPs were detected among 18 <I>Dendrobium</I> mutants and cultivars. </LI> <LI> 18 SNPs were successfully converted into KASP genotyping assays. </LI> <LI> These marker sets would be useful for identification of cultivars and the selection of elite genotypes for <I>Dendrobium</I> breeding programs. </LI> </UL> </P>

Effect of Electron Beam on in vitro Cultured Orchid (Cymbidium) Organs

Jaihyunk Ryu,Hyun-Su So,Hyun Suk Kang,Byung Cheol Lee,Chang-Hyu Bae 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04

Ionizing radiations have been effective mutagen sources to overcome the limitation of the useful genetic resources in natural environment. The study was conducted to investigate an effect of electron beam on organogenesis, growth patterns and genetic variation in the irradiated orchid organs (rhizome and protocorm). The in vitro cultured rhizomes of orchids (Cymbidium goeringii, C. kanran) were irradiated with the electron beam in the dose range of 15Gy to 2240Gy under the condition of various beam energy and beam current (10MeV/n and 0.5mA, 10MeV/n and 0.05mA, 2MeV/n and 0.5mA). Significant decreases in growth and organogenesis were observed by increase of intensity of electron beam irradiation. The irradiation intensity of lethal dose 50 (LD50) of the in vitro cultured orchid was estimated as approximately 500Gy to 1000Gy under 10MeV/n, and 1000Gy to 2000Gy under 2MeV/n. While, the optimal irradiation dose was approximately 100Gy for growth and organogenesis of the cultures under 10MeV/n with 0.05mA treatment, and 300Gy under 2MeV/n and 0.5mA electron beam condition. RAPD and ISSR analyses for the electron beam irradiated organs were performed to analyze genetic variation under the electron beam condition (2MeV/n and 0.5mA).

Comparison of Phytochemicals and Antioxidant Activity in Blackberry (Rubus fruticosus L.) Fruits of Mutant Lines at the Different Harvest Time

( Jaihyunk Ryu ),( Soon Jae Kwon ),( Yeong Deuk Jo ),( Chang Hyun Jin ),( Bo Mi Nam ),( Seung Young Lee ),( Sang Wook Jeong ),( Seung Bin Im ),( Seung Cheol Oh ),( Lan Cho ),( Bo Keun Ha ),( Si Yong K 한국육종학회 2016 Plant Breeding and Biotechnology Vol.4 No.2

Blackberries (Rubus fruticosus L.) are a good source of antioxidants and contain appreciable levels of functional compounds. This study was carried out to evaluate fruit qualities (pH, soluble solids content, titratable acidity, and minerals), functional compounds (total phenolic content, total flavonoid content, anthocyanins, and ellagic acid), and antioxidant activity among five mutated blackberry lines over three ripening stages to examine the effect of fruit maturation on functional compounds and antioxidant activity. The content of sugars, total phenolic content, total flavonoid content, and anthocyanins (cyanidin-3-O-glucoside, cyanidin-3-O-xyloside, cyanidin-3-O-malonylglucoside, and cyanidin-3-O-dioxalylglucoside) increased as fruit development advanced, whereas the ellagic acid content decreased as the fruit became more mature. Among the minerals tested, potassium showed the highest concentrations at all stages of development. The antioxidant activity was correlated with total phenolic content (P≤0.01), total flavonoid content (P≤0.01), and total anthocyanins (P≤0.05). The results indicate that Maple and B201 lines contained the highest anthocyanin content and exhibited greater antioxidant activity.