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RISS 인기검색어
- 검색키워드
- 저자 : ( Igor Kovalchuk ) (검색결과 5 건)
- 무료
- 기관 내 무료
- 유료
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Igor Kovalchuk,Priti Maheshwari 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.2
The frequency of plant transformation can beimproved by addition of various chemical into transformationmedia. In the past, we showed that exposure oftobacco, wheat and triticale explants to ammonium nitrate,cerium and lantanium chloride and potassium chlorideresulted in an increase in the frequency of transformation. Here, we tested whether a combination of increased concentrationsof the aforementioned salts yielded a highertransformation frequency. We found that exposure to61.8 mM ammonium nitrate caused a 5.0-fold increase intransformation frequency, whereas exposure to 1.0 lMcerium chloride or 47.0 mM potassium chloride resulted in1.2- and 2-fold increases, respectively. Exposure to61.8 mM ammonium nitrate and 1.0 lM cerium chlorideled to a 4.8-fold increase in transformation frequency,whereas exposure to 61.8 mM ammonium nitrate and47.0 mM potassium chloride let to a 5.2-fold increase. Finally, exposure to 61.8 mM ammonium nitrate, 1.0 lMcerium chloride and 47.0 mM potassium chloride produceda 5.1-fold increase. The analysis of the intactness ofT-DNA borders showed that plants exposed to ammoniumnitrate and a combination of ammonium nitrate with othersalts had the more intact right borders and the less intactleft borders. The best results were observed when all threesalts (ammonium nitrate, potassium chloride and ceriumchloride) were used. Thus, we concluded that the additionof cerium chloride and potassium chloride does not substantiallyimprove the transformation rate beyond theimprovement observed upon treatment with 61.8 mMammonium nitrate, but may slightly improve the intactnessof T-DNA borders.
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Neural Networks-Based Method for Electrocardiogram Classification
Maksym Kovalchuk,Viktoriia Kharchenko,Andrii Yavorskyi,Igor Bieda,Taras Panchenko International Journal of Computer ScienceNetwork S 2023 International journal of computer science and netw Vol.23 No.9
Neural Networks are widely used for huge variety of tasks solution. Machine Learning methods are used also for signal and time series analysis, including electrocardiograms. Contemporary wearable devices, both medical and non-medical type like smart watch, allow to gather the data in real time uninterruptedly. This allows us to transfer these data for analysis or make an analysis on the device, and thus provide preliminary diagnosis, or at least fix some serious deviations. Different methods are being used for this kind of analysis, ranging from medical-oriented using distinctive features of the signal to machine learning and deep learning approaches. Here we will demonstrate a neural network-based approach to this task by building an ensemble of 1D CNN classifiers and a final classifier of selection using logistic regression, random forest or support vector machine, and make the conclusions of the comparison with other approaches.
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Maheshwari, Priti,Kovalchuk, Igor 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.2
The frequency of plant transformation can be improved by addition of various chemical into transformation media. In the past, we showed that exposure of tobacco, wheat and triticale explants to ammonium nitrate, cerium and lantanium chloride and potassium chloride resulted in an increase in the frequency of transformation. Here, we tested whether a combination of increased concentrations of the aforementioned salts yielded a higher transformation frequency. We found that exposure to 61.8 mM ammonium nitrate caused a 5.0-fold increase in transformation frequency, whereas exposure to 1.0 ${\mu}M$ cerium chloride or 47.0 mM potassium chloride resulted in 1.2- and 2-fold increases, respectively. Exposure to 61.8 mM ammonium nitrate and 1.0 ${\mu}M$ cerium chloride led to a 4.8-fold increase in transformation frequency, whereas exposure to 61.8 mM ammonium nitrate and 47.0 mM potassium chloride let to a 5.2-fold increase. Finally, exposure to 61.8 mM ammonium nitrate, 1.0 ${\mu}M$ cerium chloride and 47.0 mM potassium chloride produced a 5.1-fold increase. The analysis of the intactness of T-DNA borders showed that plants exposed to ammonium nitrate and a combination of ammonium nitrate with other salts had the more intact right borders and the less intact left borders. The best results were observed when all three salts (ammonium nitrate, potassium chloride and cerium chloride) were used. Thus, we concluded that the addition of cerium chloride and potassium chloride does not substantially improve the transformation rate beyond the improvement observed upon treatment with 61.8 mM ammonium nitrate, but may slightly improve the intactness of T-DNA borders.
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( Alicja Ziemienowicz ),( Seyed Mohammad Reza Rahavi ),( Igor Kovalchuk ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.5
The single-stranded DNA binding activity of the Escherichia coli RecA protein is crucial for homologous recombination to occur. This and other biochemical activities of ssDNA binding proteins may be affected by various factors. In this study, we analyzed the effect of CaCl2, NaCl and NH4NO3 salts in combination with the pH and nucleotide cofactor effect on the ssDNA-binding activity of RecA. The studies revealed that, in addition to the inhibitory effect, these salts exert also a stimulatory effect on RecA. These effects occur only under very strict conditions, and the presence or absence and the type of nucleotide cofactor play here a major role. It was observed that in contrast to ATP, ATPγS prevented the inhibitory effect of NaCl and NH4NO3, even at very high salt concentration. These results indicate that ATPγS most likely stabilizes the structure of RecA required for DNA binding, making it resistant to high salt concentrations. [BMB reports 2011; 44(5): 341-346]
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Gene delivery into the plant mitochondria via organelle-specific peptides
Trevor MacMillan,Alicja Ziemienowicz,Fengying Jiang,François Eudes,Igor Kovalchuk 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.1
We report a method for genetically engineering the mitochondria of plant cells. Several peptides selected from sorting signal sequences of plant mitochondrial proteins were tested for cell-penetrating, specific organelle-targeting and nucleic acid-binding properties. Selected sequences were named mitochondrial targeting peptides (mTPs). Five of them were used to deliver a linear dsDNA gene construct into AC Ultima spring triticale (X. Triticosecale Wittmack) protoplasts and microspores. The mitochondrial transient expression of the aadA:gfp reporter gene was qualitatively determined with confocal microscopy and quantitatively measured using qRT-PCR. Significant aadA:gfp transcript abundance was reported in protoplasts from deliveries of all five mTPs, while this transcript abundance remained low in microspores. mTP1 transfected microspores were cultured to produce green plantlets and screened for aadA:gfp by endpoint PCR. Targeted gene integration into the mitochondrial genome of eight regenerated haploid triticale lines was confirmed by sequencing. This represents the first report of a successful in vivo transfection of crop plant somatic and germ cells via mitochondrial peptides and a method that can be used to study transient gene expression and to stably deliver genes into the mitochondria of a plant.
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