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      • 매립가스 및 폐플라스틱의 친환경 분해를 통한 수소·탄소 활용 네트워크 구축

        한효림 ( Hyorim Han ),성인호 ( In-ho Seong ),서원덕 ( Wondeok Seo ),조은정 ( Eunjeong Cho ),백주은 ( Jueun Baek ),송태진 ( Tai-jin Song ) 충북대학교 건설기술연구소 2023 建設技術論文集 Vol.42 No.2

        The current method used to recycle waste, which is responsible for more than 30% of methane generation, has limitations, including the combustion stage, which can cause additional environmental pollution during the recycling process. In addition, as the amount of waste synthetic resin entering landfills increases due to the activation of delivery services, the government has advocated the need to minimize the incineration and landfilling of waste synthetic resin. In this study, we propose 1) an eco-friendly way to resource landfill gas and waste synthetic resin generated in landfills by introducing a pyrolysis method using Ni-based catalysts, and 2) a network system that can environmentally utilize hydrogen, carbon, and carbon dioxide generated through the pyrolysis method. Finally, 3) we attempted to quantitatively calculate the environmental benefits of applying the proposed network to the Cheongju Metropolitan Landfill. Accordingly, a plan to utilize Ni-based catalysts, which have a methane conversion rate of about 1.5 times that of Si-based catalysts, was proposed, and the hydrogen generated in the process can be utilized as a fuel cell, and carbon and carbon dioxide can be utilized by establishing a carbon/carbon dioxide industry cluster around the landfill, which can bring environmental and economic benefits to the local government. As a result of applying the proposed network to the Cheongju Metropolitan Landfill, it was found that using hydrogen fuel cells to operate vehicles in the intercity bus terminal can reduce the use of fossil fuels by about 12,846.2 liters per day, which can reduce carbon dioxide by about 67 tons per month. The study also showed that if a C/CO2 industry cluster is built using the surrounding land, it can be used to reduce dependence on high carbon imports and lay the foundation for building a carbon-independent city. The results of this study can be applied to other local governments with landfills and are expected to help them achieve carbon neutrality and build carbon-independent cities.

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        Rapid, sensitive and specific detection of Brucella canis by real-time PCR assay using HybProbe

        ( Sung Ll Kang ),( Moon Her ),( Ji Yeon Kim ),( Kichan Lee ),( Jong Wan Kim ),( Hyang Keun Lee ),( So Ra Sung ),( Hyorim Cho ),( Suk Chan Jung ) 한국예방수의학회(구 한국수의공중보건학회) 2013 예방수의학회지 Vol.37 No.1

        We designed and evaluated a specific HybProbe set for identification of Brucella canis, which is based on a specific single nucleotide polymorphism (SNP) of the omp25 gene. Real-time PCR assay using this probe specifically identified B. canis reference and field strains, however, the other Brucella species and general bacteria strains were not amplified. This real-time PCR assay can re-confirm specific amplification in melting curve analysis. The average melting temperature values for generation of B. canis strains wan 67℃m, due to a single base-pair mismatch at the binding site of the probe. The detection limit appeared to be 30 fg of DNA and 100CFU by serial dilution of whole blood inoculated with B. canis. In addition, use of the buffy coat resulted in approximately 50 times higher sensitivity than whole blood directly. This technique is widely applicable for use in diagnostic laboratories and is readily discriminative with regard to small amounts of biomass.

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