Aflatoxin B1의 면역억제작용

문은이,이동권,표석능 성균관대학교 약학연구소 1996 成均藥硏論文集 Vol.8 No.1

Aflatoxin B1 (AFB1) has been reported to directly suppress the immune responses. In the present study, the effect of AFB1 on immune functions was investigated. Splenic lymphocytes were treated with various doses of the mitogens (lipopolysaccharide, concanavalin A) in the presence of AFB1. AFB1 pretretment decreased the number of plaque forming cells (PFC) in a dose-dependent manner. Antibody production of IgM and IgG class was significantly decreased in AFB1-treated splenic cells. In addition, when animals were exposed to AFB1, the susceptibility of bacterial infection as well as the growth of tumor cells was increased. These data suggest that AFB1 affected the immune function and humoral immunity impaired by AFB1 treatment contributed to pathological process.

결장직장암 환자의 임상적 고찰 : 조선대학교 부속병원의 10년 간의 결과

나은종,문성표,장정환,김권천,민영돈,김성환,조현진,김정용,김경종 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Background and Objectives: The colorectal cancer rates 4th in the total cancer prevalence in Korea. The clinical patient demographics were considered with the incidence rate of the colon and rectal cancer. The clinicopathological factors were analyzed for the 5 year survival rate. Materials Methods: Of the 282 patients who were operated from Jan. 1, 1992 to Dec. 31, 2001 and diagnosed by pathological biopsy as colo-rectal cancer, 234 patients were followed up. The age, sex, location of the cancer, TNM stage, operation method, histopathological classification, metastatic region, complications after operation and the survival rate were investigated. Results: Of the 234 patients, male and female consisted of 120 cases (51.3%) and 114 cases (46.7%), with an average age of 61.6 years. The average follow up period was 52.3 months and the peri operative mortality was 5.6%. The site of the cancer was most frequent at the rectum with 127 cases (54.3%). The patients were classified according to the TNM stages with 38 cases of stage 1 (16.2%), 98 cases of II (41.9%), 72 cases of III (30.8%) and 26 cases of IV (11.1%). The 5 year survival rate in the age group older than 50 was 63.3 % and 73.5% in the group younger than 50. In regard to the operation type, the 5 year survival rate was 65.4% for elective operation and 65.8% for emergency operation. The 5 year survival rate for well differentiated, moderately differentiated, poorly differentiated cancer, and mucinous adenocarcinoma was 73.7%, 60.2%, 58.3%, and 92.6%, respectively. The 5 year survival rate of each of the TNM stage is 81.1%, 74.8%, 57.0%, and 11.5% for Classes I, II, III, and IV, respectively. Conclusion: The clinical factors were analyzed for the 5 year survival rate with no statistical significance for the age, sex, and location of the cancer. The TNM stage and histopathological differentiation were statistically significant. The colon cancer showed better prognosis compared to the rectal cancer.

Isolation and characterization of cDNAs for Ribulose Bisphosphate Carboxylase Multigene Family in Rice (Oryza Sativum)

Moon, Eun Pyo,Lee, Kwang-Woong,Lee, Jon Seob,Choi, Yang Do,Kim, Han-Jip Korean Society of Molecular Biology 1991 Molecules and cells Vol.1 No.3

Immunomodulating activities of water extract from xanthium strumarium 2

Moon, Eun-Yi,Park, Seung-Yong,Ahn, Mee-Ja,Ahn, Jong-Woong,Zee, Ok-Pyo,Park, Eun-kyue The Pharmaceutical Society of Korea 1991 Archives of Pharmacal Research Vol.14 No.3

One of water and/or methanol extracts from 14 herbal deugs which were screened using murine splenocytes showed immunosuppressive activities previously. After water extract from Xanthium strumarium was treated with chloroform. $100 \mu{g/ml}$ of water layer (XS-WCI) has very strong immunosimulating activities tested by $^3H$-thmidine incorporation (control as $100 \mu{g/ml}$, 26345 cpm was 69515 cpm). MLR also appears to be simulated strongly (control vs $100 \mu{g/ml}$, 4962 cpm vs 78688 cpm). When $100 \mu{g/ml}$ of XS-WCI and $0.8 \mu{g/ml}$ of concanavalin a (ConA) were added. more $^3H$-thymidine were incorporated significantly, compared with $0.8 \mu{g/ml}$ of ConA only. In contrast with ConA. results from $5 \mu{/ml}$ of lipopolysaccharide (LPS) and $100 \mu{g/ml}$ of XS-WCI were not different. compared with $5\mu{/ml}$of LPS only. These results indicated the responses of XS-WCI to B cell and T cell may be different. XS-WCI was injected intraperitoneally (10 mg/kg. 50mg/kg/ 100 mg/kg) for 4 days or 10 days and tested secretion of IgM or IgG by direct and indirect hemolytic plaque-forming cell assays, respectively. Numbers of hemolytic plaques for both IgM and IgG were increased significantly. Especially, secretion of IgGs was increased more than 10 times. After administration of XS-WCI for 7 days (50 mg/kg. 100 mg/kg) splenomegaly deu to graft vs host reaction was observed. Human lymhocytes separated from whole blood by Ficoll-Hypaque method were also proliferated after treatment of $10 \mu{g/ml}$ and $50 \mu{g/ml}$ of XS-WCI. As seen in murine lymphocytes, human lymphocyte proliferation was increased synergistically after treatment with both of XS-WCI and phytohemagglutinin (PHA). It appears that XS-WCI may have potential immunosimulating activities and that it remains to be purified further for isolation of active components.

Genetic Modification of β - glucuronidase Gene for the Better Reporter

Moon, Eun Pyo 한국유전학회 1990 Genes & Genomics Vol.12 No.4

E. coli β-glucuronidase (GUS) gene is a very useful reporter in molecular biology; 1) there is a very little background in most of plant and animal tissues, ii) GUS enzyme generates very distinctive and stable blue color product upon the substrate, methylum-belliferyl-β-D-glucuronide, iii) the GUS assay is so sensitive that even the GUS activity produced by a single protoplast can be assayed. I have constructed three plasmids which have GUS gene in three different reading frames, respectively. They have several properties which make them very convenient in constructing a transcriptional and translational fusion plasmid to study the expression of a eukaryotic gene. The translational fusion of GUS also can be expressed by T₃ promoter in vitro using T₃ RNA polymerase. GUS protein is glycosylated when it is transported through ER-Golgi system, and as a result, it loses its activity. To circumvent this problem, I have modified the GUS gene using a site-directed mutagenesis to block the glycosylation site in the GUS coding region. Genetically modified GUS genes are being tested by transient expression using a particle gun. The glutelin sorting signal is used to transport the modified GUS through ER-Golgi ststem in this test.

ISOLATION AND CHARACTERIZATION OF REPETITIVE DNA SEQUENCES FROM RICE (Oryza sativa L.)

Moon, Eun-Pyo Plant molecular biology and biotechnology research 1992 Proceedings the 2nd Korean-Germany joint symposium Vol.1992 No.-

한국인의 방사선학적 골관절염 관련 위험요인

표은영(Eun-Young Pyo),조은실(Eun-Sil Cho),정문희(Moon-Hee Jung) 한양대학교 고령사회연구원 2013 한양고령사회논집 Vol.4 No.2

본 연구에서는 2010년 국민건강영양조사에 참여한 만50세 이상 대상자중 골관절염 검사에 참여한 총 3,073명(남자 1,334명, 여자 1,739명)을 대상으로 무릎과 엉덩관절의 방사선학적 골관절염 유병률과 관련 위험요인을 파악하고자 하였다. 만50세 이상에서 방사선학적 골관절염 유병률은 남자 27.9%, 여자 47.4%로 남자보다 여자의 유병률이 높았다. 남녀 모두에서 비만인 경우, 고혈압을 앓고 있는 경우 골관절염의 위험이 유의하게 높았다. 특히 여자는 육체노동자인 경우가 그렇지 않은 경우 보다 골관절염의 위험이 유의하게 높았다. 연구 결과 만50세 이상 한국 성인에서 여성, 비만, 고혈압, 육체노동이 골관절염 관련 위험요인으로 파악된다. 이러한 연구 결과를 바탕으로 추후 골관절염 예방 및 치료를 향상시키기 위한 전략 개발에 근거를 제공할 수 있을 것으로 기대한다. Objective : This study was conducted to investigate the prevalence of radiographic osteoarthritis (ROA) of hip and knee and identify risk factors. Method : The data were collected from the fifth Korea National Health and Nutrition Examination Survey (KNHANES V-1), 2010 which was a nationwide, cross-sectional survey. The study subjects were 3,073 (men 1,334, women 1,739) participants aged 50 years or above. ROA was defined as the presence of Kellgren and Lawrence grade ≥2 in either knee or hip. Data were analysed using SPSS version 18.0. Results : The prevalence of ROA was 38.4%. Women had the much higher prevalence of ROA than men (47.4% vs 27.9%). Multivariate analysis revealed that obesity and the presence of hypertension were significantly associated with the ROA in women. Conclusions : The study found that ROA is common in the aged adult population of Korea, with preponderance for women. Obesity, hypertension and manual occupation were significantly associated with the presence of ROA. Therefore, considering these factors is helpful to give some evidence for developing a strategy to improve the prevention and treatment of ROA.

임상연구 : 어려운 기관내삽관이 예측되는 상황에서 초보자에 의한 삽관 시 변형된 후두경 날이 치아 손상의 위험성에 미치는 영향

문영은 ( Young Eun Moon ),김창재 ( Chang Jae Kim ),김정은 ( Jeong Eun Kim ),홍상현 ( Sang Hyun Hong ),전준표 ( Jun Pyo Jeon ),정현도 ( Hyun Do Jung ),이재민 ( Jae Min Lee ) 대한마취과학회 2008 Korean Journal of Anesthesiology Vol.55 No.5

Background: Dental trauma is one of the most common complications during laryngoscopy, especially by novice. As the chance of making direct contact with the teeth is decreased during laryngoscopy, the chance of applying direct pressure to the teeth is decreased, thus the injury by the blade also can be decreased. The purpose of this study was to determine the effectiveness of a modified Macintosh blade on reducing dental contact and the risk of dental trauma by novice laryngoscopists in anticipated difficult airway. Methods: Sixty-six patients scheduled for elective surgery were divided into Easy group and Difficult group according to Wilson`s risk sum score. Laryngoscopy was performed twice on each patient by novice, once with a regular Macintosh 3 blade and once with a blade in which the flange was partially removed (Callander modification). The distance between the flange of the blade and the upper incisors at glottic exposure was measured. We compared the blade-tooth distances and the chance of directly contacting the tooth between two blades. Results: The modified blade provided more distance than the regular Macintosh blade in both group (P<0.001). It is also associated with decreased chance of directly contacting the teeth, especially in Difficult group (73.7% with regular blade vs 10.6% with the modified blade) (P<0.001). Conclusions: The modified Macintosh blade used in this study proved to be an effective device for novice laryngoscopists in reducing likelihood of dental injuries in anticipated difficult intubation. (Korean J Anesthesiol 2008;55:549~53)

Neuroprotective Effects of a Novel Peptide Purified from Venison Protein

( Eun Kyung Kim ),( Seung Jae Lee ),( Sang Ho Moon ),( Byong Tae Jeon ),( Bo Kyung Kim ),( Tae Kyu Park ),( Ji Sook Han ),( Pyo Jam Park ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.4

Immunomodulating Activity of DW-116, A New Quinolone Antibiotic

Moon, Eun-Yi,Choi, Chung-Ha,Pyo, Suh-Kneung,Chung, Yong-Ho,Yoon, Sung-June,Lee, Dug-Keun The Pharmaceutical Society of Korea 1998 Archives of Pharmacal Research Vol.21 No.5

DW-116, [1-(5-fluoro-2-pyridyl)-6-fluoro-7-(4-methyl-1-piperazinyl)-1,4-dihydro-4-oxoquino-line-3-carboxylic acid hydrochloride}, is a new quinolone antibiotic with a broad antibacterial spectrum against G(+) and G(-) bacteria. DW-116 was evaluated for the immunomodulating activities, which is one of the efforts to investigate the mechanism of action related to the good in vivo antibacterial efficacy. The results of in vitro studies revealed there was no statistically significant increase in B and T lymphocyte proliferation. But the results of in vivo studies showed that the number of plaque forming cells (PFC), the amount of polyclonal antibodies and delayed-type hypersensitivity (DTH) were significantly increased after the repeat administration with 12 and 60 mg/kg of DW-116. Taken together, these results proposed that immunostimulting effect of DW-116 could be one of the action mechanisms for demonstrating in vivo antibacterial activities under these experimental conditions.