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Effect of bed configuration of immobilized biocatalysts on penicillin G hydrolysis efficiency
Anca-Irina Galaction,Alexandra Cristina Blaga,Ramona Mihaela Matran,Dan Caşcaval 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.2
The external and internal mass transfer of Penicillin G in the process of its enzymatic hydrolysis to 6-Aminopenicillanicacid under competitive and non-competitive inhibitions have been comparatively analyzed for a bioreactorwith mobile bed vs. a stationary basket bioreactor, both with Penicillin amidase immobilized in Eupergit C. ThePenicillin G mass transfer and hydrolysis enzymatic rates have been analyzed by means of the ratios’ values betweenthe oxygen mass transfer coefficients, effectiveness factors, external mass flows and Penicillin G concentrations at thebiocatalyst particle surface for the considered bioreactors. The results indicated that the bioreactor with mobile bed ismore efficient especially for biocatalyst particles with diameter under 1.5 mm. For larger particles the performances ofthe two bioreactors become similar. Moreover, taking into consideration the external mass flow of Penicillin G and thenumber of enzymatic hydrolysis cycles, the basket bioreactor is recommended. The mathematical equations proposedare in good concordance with the experimental results, the average deviations varying from ±4.11% for the bioreactorwith mobile bed of immobilized Penicillin amidase to ±5.03% for the basket bioreactor.
( Galaction Anca Irina ),( Roxana Rotaru ),( Lenuta Kloetzer ),( Anestis Vlysidis ),( Colin Webb ),( Marius Turnea ),( Dan Ca Caval ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.12
This paper is dedicated to the study on the external and internal mass transfers of glucose for succinic acid fermentation under substrate and product inhibitions using a bioreactor with stirred bed of immobilized Actinobacillus succinogenes cells. By means of the substrate mass balance for a single particle of biocatalysts, considering the kinetic model adapted for both inhibitory effects, specific mathematical models were developed for describing the profiles of the substrate concentration in the outer and inner regions of biocatalysts and for estimating the substrate mass flows in the liquid boundary layer surrounding the particle and inside the particle. The values of the mass flows were significantly influenced by the internal diffusion velocity and rate of the biochemical reaction of substrate consumption. These cumulated influences led to the appearance of a biological inactive region near the particle center, its magnitude varying from 0 to 5.3% of the overall volume of particles.