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Siregar Luthfi Aziz Mahmud,Chan Lai-Keng,Boey Peng-Lim The Korean Society of Plant Biotechnology 2003 Plant molecular biology and biotechnology research Vol.5 No.2
Callus and cell suspension cultures of Eurycoma longifolia Jack were initiated from leaves of different trees. The leaf explant of tree Eu9 produced the most calli and also induced high cell biomass in the cell suspension culture. Optimum production of cell biomass could be initiated in proliferating culture medium with a pH of 5.75 prior to autoclaving. The effects of macronutrient inorganic salts of Murashige and Skoog (MS) liquid medium supplemented with X on production of cell biomass of Eurycoma longifolia were also investigated. The highest cell biomass was produced in MS medium containing macronutrients of $21\;mM\;NH_4NO_3,\;12.25\;mM\;KNO_3,\;3.00\;mM\;CaCl_2.2H_2O,\;0.575\;mM\;MgSO_4.7H_2O$, and $1.83\;mM\;KH_2PO_4$. A new medium labeled as TAM was formulated for the production of Eurycoma longifolia cell biomass in the cell suspension culture.
( Chan Chee Weng ),( Chan Lai Keng ),( Boey Peng Lim ) 한국식물학회 2004 Journal of Plant Biology Vol.47 No.3
Juvenile hormone Ⅲ (JH Ⅲ) is one of the five closely related sesquiterpenoid compounds important in the regulation of an insect physiological processes in both the developing and the mature insect. JH Ⅲ and its biosynthetic intermediates, farnesol and methyl farnesoate were detected in the in vitro Cyperus aromaticus plantlets and the mature plants growing in the field. A higher amount of methyl farnesoate (52.7%) and farnesol (31.4%) were found to be present in the in vitro plantlets as compared to the field grown mother plants which contained lower methyl farnesoate (19.5%) and farnesol (14.8%). On the other hand, the mature mother plant (4 months old) contained a higher percentage of JH Ⅲ than the in vitro plantlets. The estimated content of JHⅢ analyzed by gas chromatography indicated that the amount of JHⅢ in the 10 weeks old in vitro plantlets and the four months old mature plants of C. aromaticus was 0.076 ㎍/g and 0.085 ㎍/g respectively. This study indicated that in vitro culture system could be a potential tool for the production of this natural derived bio-insecticide and could provide a material source for further investigations of the biosynthesis of JH Ⅲ in C. aromaticus.