Effect of Tissue Factor on Invasion Inhibition and Apoptosis Inducing Effect of Oxaliplatin in Human Gastric Cancer Cell

Yu, Yong-Jiang,Li, Yu-Min,Hou, Xu-Dong,Guo, Chao,Cao, Nong,Jiao, Zuo-Yi Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Objective: Tissue factor (TF) is expressed abnormally in certain types of tumor cells, closely related to invasion and metastasis. The aim of this study was to construct a human gastric cancer cell line SGC7901 stably-transfected with human TF, and observe effects on oxaliplatin-dependent inhibition of invasion and the apoptosis induction. Methods: The target gene TF was obtained from human placenta by nested PCR and introduced into the human gastric cell line SGC7901 through transfection mediated by lipofectamine. Stably-transfected cells were screened using G418. Examples successfully transfected with TF-pcDNA3 recombinant (experimental group), and empty vector pcDNA3 (control group) were incubated with oxaliplatin. Transwell chambers were used to show change in invasive ability. Caspase-3 activity was detected using a colorimetric method and annexin-V/PI double-staining was applied to detect apoptosis. Results: We generated the human gastric cancer cell line SGC7901/TF successfully, expressing TF stably and efficiently. Compared with the control group, invasion increased, whereas caspase-3 activity and apoptosis rate were decreased in the experimental group. Conclusion: TF can enhance the invasive capacity of gastric cancer cells in vitro. Its increased expression may reduce invasion inhibition and apoptosis-inducing effects of oxaliplatin and therefore may warrant targeting for improved chemotherapy.

Volatiles from the Maillard Reaction of L-Ascorbic Acid and L-Alanine at Different pHs

Ai-Nong Yu,Qi-Hui Deng 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.6

The volatiles formed from the reactions of L-ascorbic acid with L-alanine at 5 different pH (5, 6, 7, 8, or 9) and 140±2℃ for 2 hr was performed using solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) analysis were identified to be 25 different kinds. The reaction between L-ascorbic acid and L-alanine led mainly to the formation of pyrazines. Many of these were alkylpyrazines, such as 3-ethyl-2,5-dimethylpyrazine, 2,5-dimethylpyrazine, 2-ethyl-5-methylpyrazine, 3,5-diethyl-2-methylpyrazine, methylpyrazine, 2-ethyl-6-methylpyrazine, and 2,3-diethyl-5-methylpyrazine, other compounds identified were furans, phenols, benzoquinones, 2,4,6-trimethylpyridine, and 2-methylbenzoxazole. The studies showed that furans, such as furfural and benzofuran were formed mainly at acidic pH. In contrast, higher pH values could promote the production of pyrazines.

성별에 따른 클래식음악 공연에 대한 중국소비자의 선택속성: IPA기법 중심으로

사우농(Shi, Yu-Nong),허식(Heo, Shik) 한국산업경제학회 2020 산업경제연구 Vol.33 No.6

본 연구는 중국의 클래식음악 공연상품에 있어 어떠한 선택속성을 부각해야 할지를 제시하기 위하여, 공연 선택속성의 중요도와 만족도의 관계와 각 속성별 만족도가 전반적 만족에 미치는 영향을 분석하였다. 특히, 성별에 따른 선택속성의 중요도 및 만족도의 차이를 분석하여 남성과 여성 관람객에게 어떠한 속성을 더 강조해야 할지에 대한 정보를 제공하고자 한다. 이를 위하여 T-test, IPA분석 및 회귀분석을 시행하였다. 연구의 주요결과는 다음과 같다. 첫째, 중국에서 클래식음악공연 선택속성의 중요도는 작품내용, 공연장, 프로모션, 공연가격, 성악가 순으로 동일하게 나타났으나, 만족도의 경우 남녀가 상이하게 나타났다. 둘째, 중요도는 높으나 만족도가 저조하여 개선이 필요한 집중항목의 경우에 남성은 공연시설, 티켓 할인, 입장권가격, 공연정보제공으로 분석되었으며, 여성은 성악가경력, 성악가외모, 공연장규모, 편의시설로 분석되어 성별에 따라 개선이 필요한 속성이 다르게 나타났다. 마지막으로 중국 클래식음악 공연의 전반적 만족은 여성이 남성에 비해 높게 나타났으며, 선택속성 중 작품내용, 공연장, 프로모션의 만족도가 전반적 만족에 영향을 주는 것으로 나타났다. 따라서 본 연구는 향후 중국 클래식음악 공연시장에 이해와 중국시장으로의 진출 시 의미 있는 기초자료로 사용될 수 있다. The purpose of this study is to examine the importance and satisfaction of the selection attributes according to gender of classical music performance, in which visitors are late for Chinese classical music performances, and to provide information on what attributes should be emphasized more to male and female audiences of current classical music performances. The main results of this study are as follows: First, the importance of the optional attributes of Chinese classical music performances was shown in the order of ‘performance contents’, ‘performance hall’, ‘performance promotion’, ‘performance price’ and ‘performance vocalists’. Second, in the analysis of the importance-satisfaction of men, it was analyzed that ‘the facility of the performance hall’, ‘whether tickets are discounted’, ‘the price of admission tickets’ and ‘the provision of Internet information on the performance’ were concentrated. Third, in the analysis of women’s importance-satisfaction, they were analyzed as ‘the career of a vocalist’, ‘the appearance of a vocalist’, ‘the scale of the concert hall’ and ‘the facilities around the concert hall’ which require intensive improvement. Finally, for Chinese classical music performance audiences, there is a significant difference in overall satisfaction with the attributes of selection of classical music performances, and individual selection attributes affect overall satisfaction, such factors as “performance promotion,” “performance content” and “performance hall.” This study analyzes the consumer selection attributes of Chinese classical music performance and is judged to be a basic marketing material that is meaningful when understanding and entering the Chinese classical music performance segmentation market in the future.

Comparative Study on the Value of Anal Preserving Surgery for Aged People with Low Rectal Carcinoma in Jiangsu, China

Yu, Dong-Sheng,Huang, Xin-En,Zhou, Jian-Nong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Objective: To compare the efficacy of anal preserving surgery for aged people with low rectal carcinoma. Methods: Clinical data for a consecutive cohort of 98 rectal cancer patients with distal tumors located within 3cm -7cm of the anal verge were collected. Among these, 42 received anal preserving surgery (35 with Dixon, 3 with Parks and 4 with transanal operations). The local recurrence and survival rates in the above operations were compared with those of the Miles operation in another 56 patients with rectal cancer. Results: The local recurrence and 3-, 5-year survival rates of anal preserving surgery were 16.7%, 64.3% and 52.4%, those of Miles operations were 16.1%, 67.9% and 51.8% respectively (P>0.05). Conclusion: Anal preserving surgery for aged people with low rectal cancer is not inferior to conventional operations in China, with satisfactory long term survival and comparable local recurrence rates.

Bio-Inspired Coloring and Functionalization of Silk Fabric via Laccase-Catalyzed Graft Polymerization of Arylamines

Yelin Nong,Zhou Zhou,Jiugang Yuan,Ping Wang,Yuanyuan Yu,Qiang Wang,Xuerong Fan 한국섬유공학회 2020 Fibers and polymers Vol.21 No.9

Generally, dyeing and finishing of Bombyx mori silk fibers were proceeded at high temperature, which has thedisadvantages of overmuch energy-consumptions. In the present work, bio-inspired in situ coloring and functionalization ofsilk fabric were carried out via laccase-catalyzed graft polymerization of arylamines, including aniline (ANI) and pphenylenediamine(PPD). p-Hydroxyphenylacetamide (PHAD) as the model compound of tyrosine residues in silk fibroin(SF) was used to explore the mechanism of graft polymerization by means of MALDI-TOF MS. The actions of the dopantsof sodium dodecyl benzene sulfonate (SDBS) and sodium p-aminobenzenesulfonate (ABSA) on the enzymatic treatmentwere also concerned. The results indicated that laccase might initiate the self-polymerizations of ANI and PPD, respectively,and formed the copolymers of arylamine and PHAD subsequently. High color depth and color fastness were obtained afterincubation silk fabric with laccase and arylamines, companying with a noticeable pH-responsiveness. The laccase-mediatedtreatment endowed silk fabric with antibacterial and antioxidant abilities, meanwhile, the addition of dopants of SDBS andABSA promoted the enzymatic finishing. Enzymatic graft polymerization of arylamine onto silk fibroin provides a novelapproach for dyeing and functionalization of silk fabrics under mild treating conditions.

Aberrant Expression of Markers of Cancer Stem Cells in Gastric Adenocarcinoma and their Relationship to Vasculogenic Mimicry

Zhou, Lei,Yu, Lan,Feng, Zhen-Zhong,Gong, Xiao-Meng,Cheng, Ze-Nong,Yao, Nan,Wang, Dan-Na,Wu, Shi-Wu Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.10

Background: Gastric cancer is the second leading cause of cancer-related death in Asia, and the majority type is gastric adenocarcinoma (GAC). Most GAC patients die of recurrence and metastasis. Cancer stem cells (CSCs) have been thought to be responsible for the initiation, development, metastasis, and ultimately recurrence of cancer. In this study, we aimed to investigate expression and clinical significance of CSCs markers, CD133 and Lgr5, and vasculogenic mimicry (VM) in primary GAC. Materials and Methods: Specimens from 261 Chinese patients with follow-up were analyzed for CD133, Lgr5 protein expression and VM by immunohistochemical and histochemical staining. The Pearson Chi's square test was used to assess the associations among the positive staining of these markers and clinicopathological characteristics. Postoperative overall survival time was were studied by univariate and multivariate analyses. Results: In GAC tissues, positive rates of 49.0%, 38.7%, and 26.8% were obtained for CD133, Lgr5, and VM, respectively. The mean score of microvessel density (MVD) was $21.7{\pm}11.1$ in GAC tissues. There was a significantly difference between the positive and negative groups. There was a positive relationship between the VM, the expression of CD133 and Lgr5, and the score of MVD and the grades of tumor, lymph node metastasis, TNM stages (all p<0.05). The overall mean survival time of the patients with CD133, Lgr5, VM, and MVD (${\geq}22$) positive expression was lower than that of patients with negative expression. The score of MVD, positive expression of CD133 and VM were independent prognostic factors of GAC (p<0.05). Conclusions: VM, and expression of CD133, Lgr5, and the score of MVD are related to grades of tumor, lymph node metastasis, TNM stages, and overall mean survival time. It is suggested that CSCs and VM could play an important role in the evolution of GAC.

Treatment Efficacy and Prognostic Factors for Huge HCC Based on Barcelona Clinic Liver Cancer Staging

Zhang, Zhi-Ming,Zhang, Yu-Mei,Gao, Sheng,Yuan, Wei-Ping,Zhao, Yin-Nong,Xiang, Bang-De,Wu, Fei-Xiang,Wu, Guo-Bin,Liu, Jian-Yong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

Objective: To explore the most appropriate treatment for patients with hepatocellular cancer (HCC) >10 cm by using the Barcelona Clinic Liver Cancer (BCLC) classification. Materials and Methods: A total of 124 HCC patients undergoing surgery were selected. Disease-free survival (DFS), overall survival (OS) and prognostic factors were respectively assessed. Results: This study showed that the cumulative 1-, 3-, 5-year survival rates were 79.7%, 59.8% and 41.6% in BCLC-A patients, 76.2%, 9.5% and 0% in BCLC-B patients and 44.9%, 0% and 0% in BCLC-C patients, respectively. The 1-, 3-, 5-year DFS rates were 49%, 24.5% and 9.1% in BCLC-A patients, 7.5%, 0% and 0% in BCLC-B patients, respectively. No BCLC-C patients survived 1 year after surgery. Multivariate analysis indicated that hepatitis B surface antigen (HBsAg), vascular invasion, intra-hepatic metastasis, curative resection, tumor rupture and pathologic differentiation were independent prognostic factors. Conclusions: Surgery is effective and safe for patients with HCC >10 cm with sufficient hepatic reserve.

Effect of the Substrate Temperatures on the Epitaxial Rearrangement of the Deposited Au Nanoclusters

Lee, S. C.,Hwang, Nong M.,Yu, B. D.,Kim, D. Y. 대한금속학회 2002 METALS AND MATERIALS International Vol.8 No.5

As the existence of charged clusters in the gas phase, which were predicted by the charged cluster model, has been confirmed for many thin film processes, it becomes important to understand the deposition dynamics of the clusters. In this study, deposition behaviors of the cluster of 1985 Au atoms on the (100) gold surface were studied at the deposition temperatures of 300, 700 and 1000 K by molecular dynamics (MD) simulation using the embedded atom method (EAM) potential. After 320 picoseconds of cluster landing on the surface at 300 and 700 K, only a part of the cluster rearranged into the epitaxial orientation with the substrate with the rest of the cluster making a grain boundary at 300 K and a twin at 700 K. At 1000 K, however, the cluster fully underwent epitaxial recrystallization. These results imply that the high rate of epitaxial deposition by clusters of a few nanometers is possible as long as the substrate temperature is sufficiently high as was previously suggested in the charged cluster model.

Chemoenzymatic Synthesis of GDP-L-Fucose Derivatives as Potent and Selective -1,3-Fucosyltransferase Inhibitors

Wei-Che Hsieh,Yu-Nong Lin,Ting-Chien Lin,Chun-Hung Hans Lin 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1

Fucosyltransferases (FucTs) usually catalyze the final step of glycosylation and are critical to many biological processes. High levels of specific FucT activities are often associated with various cancers. Here we report the development of a chemoenzymatic method for synthesizing a library of GDP-L-fucose derivatives, followed by in situ screening for inhibitory activity against bacterial and human -1,3-FucTs (4). Several compounds incorporating appropriate hydrophobic moieties were identified from the initial screening. These were individually synthesized, purified and characterized in detail for their inhibition kinetics. Compound 5 had a Ki of 29 nM for human FucT-VI, and is 269 and 11 times more selective than for Helicobacter pylori FucT (Ki = 7.8 □ M) and for human FucT-V (Ki = 0.31 □ M).

Carcinoma Microsatellite Instability Status as a Predictor of Benefit from Fluorouracil-Based Adjuvant Chemotherapy for Stage II Rectal Cancer

Yang, Liu,Sun, Yan,Huang, Xin-En,Yu, Dong-Sheng,Zhou, Jian-Nong,Zhou, Xin,Li, Dong-Zheng,Guan, Xin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.4

Purpose: Rectal cancers with high microsatellite-instable have clinical and pathological features that differentiate them from microsatellite-stable or low-frequency carcinomas, which was studied rarely in stage II rectal cancer, promoting the present investigation of the usefulness of microsatellite-instability status as a predictor of the benefit of adjuvant chemotherapy with fluorouracil in stage II rectal cancer. Patients and Methods: Data of 460 patients who underwent primary anterior resection with a double stapling technique for rectal carcinoma at a single institution from 2008 to 2012 were retrospectively collected. All patients experienced a total mesorectal excision (TME) operation. Survival analysis were analyzed using the Cox regression method. Results: Five-year rate of disease-free survival (DFS) was noted in 390 (84.8%) of 460 patients with stage II rectal cancer. Of 460 tissue specimens, 97 (21.1%) exhibited high-frequency microsatellite instability. Median age of the patients was 65 (50-71) and 185 (40.2%) were male. After univariate and multivariate analysis, microsatellite instability (p= 0.001), female sex (p<0.05) and fluorouracil-based adjuvant chemotherapy (p<0.001), the 3 factors were attributed to a favorable survival status independently. Among 201 patients who did not receive adjuvant chemotherapy, those cancers displaying high-frequency microsatellite instability had a better 5-year rate of DFS than tumors exhibiting microsatellite stability or low-frequency instability (HR, 13.61 [95% CI, 1.88 to 99.28]; p= 0.010), while in 259 patients who received adjuvant chemotherapy, there was no DFS difference between the two groups (p= 0.145). Furthermore, patients exhibiting microsatellite stability or low-frequency instability who received adjuvant chemotherapy had a better 5-year rate of DFS than patients did not (HR, 5.16 [95% CI, 2.90 to 9.18]; p<0.001), while patients exhibiting high-frequency microsatellite instability were not connected with increased DFS (p= 0.696). It was implied that female patients had better survival than male. Conclusion: Survival status after anterior resection of rectal carcinoma is related to the microsatellite instability status, adjuvant chemotherapy and gender. Fluorouracil-based adjuvant chemotherapy benefits patients of stage II rectal cancer with microsatellite-stable or low microsatellite-instable, but not those with high microsatellite-instable. Additionally, free of adjuvant chemotherapy, carcinomas with high microsatellite-instable have a better 5-year rate of DFS than those with microsatellite-stable or low microsatellite-instable, and female patients have a better survival as well.