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Magnetic-activated cell sorting improves high-quality spermatozoa in bovine semen
Teresinha Inês de Assumpção,Neimar Correa Severo,João Pedro Brandão Zandonaide,Gustavo Guerino Macedo 한국동물생명공학회(구 한국동물번식학회) 2021 Journal of Animal Reproduction and Biotechnology Vol.36 No.2
The objective of this study was to establish a selection process for high quality sperm in bovine semen using sperm separation by magnetic activation (MACS). For this, semen from 21 Nellore bulls was collected using an artificial vagina. To guarantee the presence of pathologies in the ejaculate, animals previously declassified in four consecutive spermiogram were used. Semen was analyzed in five statuses: (1) fresh semen (fresh); (2) density gradient centrifugation (DGC), percoll column; (3) non-apoptotic fraction after separation by MACS (MAC); (4) apoptotic fraction from the separation (MACPOOR); and (5) MAC followed by DGC (MACDGC). Using a computerized analysis system (CASA), motility was measured. The sperm morphology was evaluated by phase contrast, and the supravital test was completed with eosin/nigrosin staining. For DGC, 20 × 106 cells were used in a gradient of 90% and 45% percoll. MACS used 10 × 106 cells with 20 μL of nanoparticles attached to annexin V, and filtered through the MiniMACS magnetic separation column. Membrane integrity was assessed with SYBR-14/IP and mitochondrial potential with JC-1 by flow cytometry. Processing sperm by MACDGC, was more effective in obtaining samples with high quality sperm, verified by the total of abnormalities in the samples: 35.04 ± 2.29%, 21.50 ± 1.47%, 17.30 ± 1.10%, 30.68 ± 1.94% and 10.50 ± 1.46%, respectively for fresh, DGC, MAC, MACPOOR, and MACDGC. The subpopulation of non-apoptotic sperm had a high number of live cells (82.65%), membrane integrity (56.60%) and mitochondrial potential (83.98%) (p < 0.05). These findings suggest that this nanotechnological method, that uses nanoparticles, is efficient in the production of high-quality semen samples for assisted reproduction procedures in cattle.
Magnetic-activated cell sorting improves high-quality spermatozoa in bovine semen
de Assumpcao, Teresinha Ines,Severo, Neimar Correa,Zandonaide, Joao Pedro Brandao,Macedo, Gustavo Guerino The Korean Society of Animal Reproduction and Biot 2021 한국동물생명공학회지 Vol.36 No.2
The objective of this study was to establish a selection process for high quality sperm in bovine semen using sperm separation by magnetic activation (MACS). For this, semen from 21 Nellore bulls was collected using an artificial vagina. To guarantee the presence of pathologies in the ejaculate, animals previously declassified in four consecutive spermiogram were used. Semen was analyzed in five statuses: (1) fresh semen (fresh); (2) density gradient centrifugation (DGC), percoll column; (3) non-apoptotic fraction after separation by MACS (MAC); (4) apoptotic fraction from the separation (MACPOOR); and (5) MAC followed by DGC (MACDGC). Using a computerized analysis system (CASA), motility was measured. The sperm morphology was evaluated by phase contrast, and the supravital test was completed with eosin/nigrosin staining. For DGC, 20 × 10<sup>6</sup> cells were used in a gradient of 90% and 45% percoll. MACS used 10 × 10<sup>6</sup> cells with 20 μL of nanoparticles attached to annexin V, and filtered through the MiniMACS magnetic separation column. Membrane integrity was assessed with SYBR-14/IP and mitochondrial potential with JC-1 by flow cytometry. Processing sperm by MACDGC, was more effective in obtaining samples with high quality sperm, verified by the total of abnormalities in the samples: 35.04 ± 2.29%, 21.50 ± 1.47%, 17.30 ± 1.10%, 30.68 ± 1.94% and 10.50 ± 1.46%, respectively for fresh, DGC, MAC, MACPOOR, and MACDGC. The subpopulation of non-apoptotic sperm had a high number of live cells (82.65%), membrane integrity (56.60%) and mitochondrial potential (83.98%) (p < 0.05). These findings suggest that this nanotechnological method, that uses nanoparticles, is efficient in the production of high-quality semen samples for assisted reproduction procedures in cattle.
Contributed Mini Review : Stathmin 1 in normal and malignant hematopoiesis
( Joao Agostinho Machado Neto ),( Sara Teresinha Olalla Saad ),( Fabiola Traina ) 생화학분자생물학회(구 한국생화학분자생물학회) 2014 BMB Reports Vol.47 No.12
Stathmin 1 is a microtubule destabilizer that plays an important role in cell cycle progression, segregation of chromosomes, clonogenicity, cell motility and survival. Stathmin 1 overexpression has been reported in malignant hematopoietic cells and Stathmin 1 inhibition reduces the highly proliferative potential of leukemia cell lines. However, during the differentiation of primary hematopoietic cells, Stathmin 1 expression decreases in parallel to decreases in the proliferative potential of early hematopoietic progenitors. The scope of the present review is to survey the current knowledge and highlight future perspectives for Stathmin 1 in normal and malignant hematopoiesis, with regard to the expression, function and clinical implications of this protein.
Mariana T. De Souza,Gabriela Vera-Lozada,Moneeb Othman,Teresinha J. Marques-Salles,Luciana W. Pinto,Moisés M. da Rocha,Soraia Rouxinol,Thomas Liehr,Raul C. Ribeiro,Rocio Hassan,Maria Luiza Macedo Silv 대한진단검사의학회 2018 Annals of Laboratory Medicine Vol.38 No.1
Dear Editor, Burkitt lymphoma/leukemia (BL/L) was the first neoplasia associated with rearrangement in MYC that is the molecular hallmark of this disease. Particularly, immunoglobulin genes are associated with MYC in BL/L, where the juxtaposition with heavy chain locus (14q32) or light chain loci (2p12 and 22q11) leads to MYC overexpression, which is believed to play a central role in BL/L pathogenesis
Influence of Toxoplasma gondii Acute Infection on Cholinesterase Activities of Wistar Rats
Alexandre Alberto Tonin,Aleksandro Schafer da Silva,Maria Luiza Thorstenberg,Livia Gelain Castilhos,Raqueli Teresinha Franca,Daniela Bitencourt Rosa Leal,Marta Maria Medeiros Frescura,Fernanda Silveir 대한기생충학열대의학회 2013 The Korean Journal of Parasitology Vol.51 No.4