The round-the-clock technique for correction of gynecomastia

Tarallo, Mauro,Taranto, Giuseppe Di,Fallico, Nefer,Ribuffo, Diego Korean Society of Plastic and Reconstructive Surge 2019 Archives of Plastic Surgery Vol.46 No.3

Background Gynecomastia is a common condition that can cause severe emotional and physical distress in both young and older men. Patients in whom symptomatic recalcitrant gynecomastia persists for a long time are potential candidates for surgery. Methods From January 2014 to January 2016, 15 patients underwent correction of gynecomastia through a single 3-mm incision at our institution. Only patients with true gynecomastia underwent surgery with this new technique. Through the small incision, sharp dissection was performed in a clockwise and counterclockwise direction describing two half-circles. Health-related quality of life and aesthetic outcomes were evaluated using a modified version of the Breast Evaluation Questionnaire (BEQ). Results The patients' average age was 23.5 years (range, 18-28 years), and their average body mass index was $23.2kg/m^2$ (range, $19.2-25.3kg/m^2$). One case was unilateral and 14 cases were bilateral. The weight of glandular tissue resected from each breast ranged from 80 to 170 g. No excess skin was excised. Bleeding was minimal. The mean operating time was 25 minutes (range, 21-40 minutes). No complications were recorded. All lesions were histologically benign. The patients' average score was 3.5 (on a 5-point Likert scale) in all domains of the BEQ for themselves and their partners. Conclusions In this study, we demonstrated the safety and reliability of a new technique that allows mastectomy through an imperceptible 3-mm incision. We obtained high patient satisfaction scores using our surgical technique, and patients reported considerable improvement in their social, physical, and psychological well-being after surgery.

Response to Letter: Adjustments to the round-the-clock technique for correction of gynecomastia

Tarallo, Mauro,Di Taranto, Giuseppe,Fallico, Nefer,Ribuffo, Diego Korean Society of Plastic and Reconstructive Surge 2019 Archives of Plastic Surgery Vol.46 No.6

Response to Letter: Adjustments to the round-the-clock technique for correction of gynecomastia

Mauro Tarallo,Giuseppe Di Taranto,Nefer Fallico,Diego Ribuffo 대한성형외과학회 2019 Archives of Plastic Surgery Vol.46 No.6

The round-the-clock technique for correction of gynecomastia

Mauro Tarallo,Giuseppe Di Taranto,Nefer Fallico,Diego Ribuffo 대한성형외과학회 2019 Archives of Plastic Surgery Vol.46 No.3

Background Gynecomastia is a common condition that can cause severe emotional and physical distress in both young and older men. Patients in whom symptomatic recalcitrant gynecomastia persists for a long time are potential candidates for surgery. Methods From January 2014 to January 2016, 15 patients underwent correction of gynecomastia through a single 3-mm incision at our institution. Only patients with true gynecomastia underwent surgery with this new technique. Through the small incision, sharp dissection was performed in a clockwise and counterclockwise direction describing two half-circles. Health-related quality of life and aesthetic outcomes were evaluated using a modified version of the Breast Evaluation Questionnaire (BEQ). Results The patients’ average age was 23.5 years (range, 18–28 years), and their average body mass index was 23.2 kg/m2 (range, 19.2–25.3 kg/m2). One case was unilateral and 14 cases were bilateral. The weight of glandular tissue resected from each breast ranged from 80 to 170 g. No excess skin was excised. Bleeding was minimal. The mean operating time was 25 minutes (range, 21–40 minutes). No complications were recorded. All lesions were histologically benign. The patients’ average score was 3.5 (on a 5-point Likert scale) in all domains of the BEQ for themselves and their partners. Conclusions In this study, we demonstrated the safety and reliability of a new technique that allows mastectomy through an imperceptible 3-mm incision. We obtained high patient satisfaction scores using our surgical technique, and patients reported considerable improvement in their social, physical, and psychological well-being after surgery.

The efficacy of different implant surface decontamination methods using spectrophotometric analysis: an in vitro study

Roberto Gif,Davide Pietropaoli,Leonardo Mancini,Francesco Tarallo,Philipp Sahrmann,Enrico Marchetti 대한치주과학회 2023 Journal of Periodontal & Implant Science Vol.53 No.4

Purpose: Various methods have been proposed to achieve the nearly complete decontamination of the surface of implants affected by peri-implantitis. We investigated the in vitro debridement efficiency of multiple decontamination methods (Gracey curettes [GC], glycine air-polishing [G-Air], erythritol air-polishing [E-Air] and titanium brushes [TiB]) using a novel spectrophotometric ink-model in 3 different bone defect settings (30°, 60°, and 90°). Methods: Forty-five dental implants were stained with indelible ink and mounted in resin models, which simulated standardised peri-implantitis defects with different bone defect angulations (30°, 60°, and 90°). After each run of instrumentation, the implants were removed from the resin model, and the ink was dissolved in ethanol (97%). A spectrophotometric analysis was performed to detect colour remnants in order to measure the cumulative uncleaned surface area of the implants. Scanning electron microscopy images were taken to assess micromorphological surface changes. Results: Generally, the 60° bone defects were the easiest to debride, and the 30° defects were the most difficult (ink absorption peak: 0.26±0.04 for 60° defects; 0.32±0.06 for 30° defects; 0.27±0.04 for 90° defects). The most effective debridement method was TiB, independently of the bone defect type (TiB vs. GC: P<0.0001; TiB vs. G-Air: P=0.0017; TiB vs. GE-Air: P=0.0007). GE-Air appeared to be the least efficient method for biofilm debridement. Conclusions: T-brushes seem to be a promising decontamination method compared to the other techniques, whereas G-Air was less aggressive on the implant surface. The use of a spectrophotometric model was shown to be a novel but promising assessment method for in vitro ink studies.

Anti–neuropilin-1 peptide inhibition of synoviocyte survival, angiogenesis, and experimental arthritis

Kong, Jin-Sun,Yoo, Seung-Ah,Kim, Jung-Wook,Yang, Seung-Pil,Chae, Chi-Bom,Tarallo, Valeria,Falco, Sandro De,Ryu, Sung-Ho,Cho, Chul-Soo,Kim, Wan-Uk Wiley Subscription Services, Inc., A Wiley Company 2010 Vol.62 No.1

<B>Objective</B><P>To delineate the role of neuropilin-1 (NP-1), a vascular endothelial growth factor receptor (VEGFR), in rheumatoid inflammation and to determine whether blockade of NP-1 could suppress synoviocyte survival and angiogenesis.</P><B>Methods</B><P>VEGF<SUB>111–165</SUB> peptide, which encompasses the NP-1 binding domain of VEGF<SUB>165</SUB>, was generated by cleaving VEGF<SUB>165</SUB> with plasmin. The effect of this peptide on the interaction between VEGF<SUB>165</SUB> and its receptor was determined by <SUP>125</SUP>I-VEGFR binding assay. Assays to determine synoviocyte apoptosis, adhesion, and migration were performed in the presence of VEGF<SUB>165</SUB> and/or the peptide. VEGF<SUB>165</SUB>-induced angiogenesis was assessed by measuring the proliferation, tube formation, and wounding migration of endothelial cells (ECs). Mice were immunized with type II collagen to induce experimental arthritis.</P><B>Results</B><P>VEGF<SUB>111–165</SUB> peptide specifically inhibited the binding of <SUP>125</SUP>I-VEGF<SUB>165</SUB> to NP-1 on rheumatoid synoviocytes and ECs. The peptide eliminated the VEGF<SUB>165</SUB>-mediated increase in synoviocyte survival and activation of p-ERK and Bcl-2. The peptide also completely inhibited a VEGF<SUB>165</SUB>-induced increase in synoviocyte adhesion and migration. In addition, the anti–NP-1 peptide blocked VEGF<SUB>165</SUB>-stimulated proliferation, capillary tube formation, and wounding migration of ECs in vitro. VEGF<SUB>165</SUB>-induced neovascularization in a Matrigel plug in mice was also blocked by treatment with the peptide. Finally, subcutaneous injection of anti–NP-1 peptide suppressed arthritis severity and autoantibody formation in mice with experimental arthritis and inhibited synoviocyte hyperplasia and angiogenesis in arthritic joints.</P><B>Conclusion</B><P>Anti–NP-1 peptide suppressed VEGF<SUB>165</SUB>-induced increases in synoviocyte survival and angiogenesis, and thereby blocked experimental arthritis. Our findings suggest that anti–NP-1 peptide could be useful in alleviating chronic arthritis.</P>

Cancer-derived exosomal Alu RNA promotes colorectal cancer progression

Magliacane Trotta Sara,Adinolfi Antonio,D’Orsi Luca,Panico Sonia,Mercadante Grazia,Mehlen Patrick,Ambati Jayakrishna,De Falco Sandro,Tarallo Valeria 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-

Inflammation plays a crucial role in cancer progression, but the relevance of the inflammasome remains unclear. Alu RNA was the first endogenous nucleic acid shown to activate the NLRP3 (nucleotide-binding domain leucine-rich repeat containing 3) inflammasome. Here, we showed that Alu RNA can induce epithelial-to-mesenchymal transition (EMT) through NLRP3 inflammasome activation and IL-1β release in colorectal cancer (CRC) cells. Alu RNA is stored, transported and transferred to CRC cells by exosomes. Exosomal Alu RNA promotes tumorigenesis by inducing invasion, metastasis and EMT via NLRP3 inflammasome activation. Consistent with these data, we found that significantly increased Alu RNA expression correlates with the induction of NLRP3 priming in human CRC patients. Furthermore, the level of Alu RNA in circulating exosomes correlates with CRC progression in a preclinical model. These findings reveal the direct involvement of Alu RNA in cancer pathogenesis, and its presence in CRC cell-derived exosomes could be used as a noninvasive diagnostic biomarker.