Intragastrically Applicated CCI₄-Thiopental Sodium Enhanced Lipid Peroxidation and Liver Fibrosis (Cirrhosis) in Rat

Ki-Young Kim,Syung-Eun Cho,Byung-Soo Yu 한국독성학회 2009 Toxicological Research Vol.25 No.2

We investigated the pathogenesis of liver tissue damage during the lipid peroxidation and fibrogenesis with the observation of correlations between the parameters of collagen synthesis (and deposition) and lipid peroxidation in liver fibrosis (cirrhosis) rats. Rats were randomly divided into two groups, normal and CCI₄-thiopental sod. intoxicated group. And the one group was treated intragastrically with the mixture of CCI₄-thiopental sod. 3 times per week for 3 weeks. The liver tissue and sera were used for the measurement of hydroxyproline (HYP), malonedialdehyde (MDA) and superoxide dismutase (SOD). Biochemical parameters such as aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total-bilirubin and blood urea nitrogen (BUN) were measured. Additionally, the expression of collagen α1(Ⅲ) and β-actin mRNA was observed by RTPCR. The histological change in liver tissue was also observed by Masson’s trichrome and H&E staining. Correlation analysis was carried by Spearman’s rho method. All biochemical parameters except total-bilirubin were significantly higher in the CCI₄-thiopental sod. treated group than that of the normal group (p<0.01). In the CCI₄-thiopental sod. treated group, Hyp as a parameter of collagen synthesis (deposition) and MDA as a metabolite of lipid peroxidation, were significantly elevated by 1.98 and 2.11 times higher than that of the normal group (p<0.001) respectively. The activity of SOD in the CCI₄-thiopental sod. treated group is decreased significantly by 44.8% (p<0.001). And collagen α1(Ⅲ) mRNA was more expressed in the CCI₄-thiopental sod. treated group than that of the normal group. However, the expression of β-actin mRNA is showed similar in both of groups. A good correlation was observed between the content of hyp and MDA concentration (r=0.70, n=40) in the two groups. And the correlation between the levels of hyp and SOD (r=-0.71, n=25) is also reliable. However, no correlation were observed between MDA concentration and SOD (r=-0.40, n=25) in the two groups. Elevated levels of MDA in CCI₄-thiopental sod. treated rats indicated enhancement of lipid peroxidation, which is accompanied by a decrease in SOD activity. Moreover, we could confirm that the parameters of collagen synthesis (and deposition) is in good correlation with the metabolite of lipid peroxidation (MDA) and the lipid peroxidation antagonizing enzyme (SOD). Hence, we propose that ① lipid peroxidation and collagen synthesis (and deposition) could be enhanced by intragastrically application of CCI₄-thiopental sod. during a short terms. And ② the intoxication of CCI₄-thiopental sod. could be used for monitoring of lipid peroxidation and collagen synthesis (and depositon) for test of antioxidant and antifibrotic agent.

Intragastrically Applicated CCl4-Thiopental Sodium Enhanced Lipid Peroxidation and Liver Fibrosis (Cirrhosis) in Rat: Malonedialdehyde as a Parameter of Lipid Peroxidation Correlated with Hydroxyproline as a Parameter of Collagen Synthesis (Deposition)

Ki-Young Kim,Syung-Eun Cho,Byung-Soo Yu 한국독성학회 2009 Toxicological Research Vol.25 No.2

We investigated the pathogenesis of liver tissue damage during the lipid peroxidation and fibrogenesis with the observation of correlations between the parameters of collagen synthesis (and deposition) and lipid peroxidation in liver fibrosis (cirrhosis) rats. Rats were randomly divided into two groups, normal and CCl4-thiopental sod. intoxicated group. And the one group was treated intragastrically with the mixture of CCl4-thiopental sod. 3 times per week for 3 weeks. The liver tissue and sera were used for the measurement of hydroxyproline (HYP), malonedialdehyde (MDA) and superoxide dismutase (SOD). Biochemical parameters such as aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total-bilirubin and blood urea nitrogen (BUN) were measured. Additionally, the expression of collagen 1(Ⅲ) and -actin mRNA was observed by RT-PCR. The histological change in liver tissue was also observed by Masson’s trichrome and H&E staining. Correlation analysis was carried by Spearman’s rho method. All biochemical parameters except total-bilirubin were significantly higher in the CCl4-thiopental sod. treated group than that of the normal group (p < 0.01). In the CCl4-thiopental sod. treated group, Hyp as a parameter of collagen synthesis (deposition) and MDA as a metabolite of lipid peroxidation, were significantly elevated by 1.98 and 2.11 times higher than that of the normal group (p < 0.001) respectively. The activity of SOD in the CCl4-thiopental sod. treated group is decreased significantly by 44.8% (p < 0.001). And collagen 1(Ⅲ) mRNA was more expressed in the CCl4-thiopental sod. treated group than that of the normal group. However, the expression of -actin mRNA is showed similar in both of groups. A good correlation was observed between the content of hyp and MDA concentration (r = 0.70, n = 40) in the two groups. And the correlation between the levels of hyp and SOD (r = -0.71, n = 25) is also reliable. However, no correlation were observed between MDA concentration and SOD (r = -0.40, n = 25) in the two groups. Elevated levels of MDA in CCl4-thiopental sod. treated rats indicated enhancement of lipid peroxidation, which is accompanied by a decrease in SOD activity. Moreover, we could confirm that the parameters of collagen synthesis (and deposition) is in good correlation with the metabolite of lipid peroxidation (MDA) and the lipid peroxidation antagonizing enzyme (SOD). Hence, we propose that ① lipid peroxidation and collagen synthesis (and deposition) could be enhanced by intragastrically application of CCl4-thiopental sod. during a short terms. And ? the intoxication of CCl4-thiopental sod. could be used for monitoring of lipid peroxidation and collagen synthesis (and depositon) for test of antioxidant and antifibrotic agent.

Intragastrically Applicated CCl₄-Thiopental Sodium Enhanced Lipid Peroxidation and Liver Fibrosis (Cirrhosis) in Rat: Malonedialdehyde as a Parameter of Lipid Peroxidation Correlated with Hydroxyproline as a Parameter of Collagen Synthesis (De

Kim, Ki-Young,Cho, Syung-Eun,Yu, Byung-Soo Korean Society of ToxicologyKorea Environmental Mu 2009 Toxicological Research Vol.26 No.2

We investigated the pathogenesis of liver tissue damage during the lipid peroxidation and fibrogenesis with the observation of correlations between the parameters of collagen synthesis (and deposition) and lipid peroxidation in liver fibrosis (cirrhosis) rats. Rats were randomly divided into two groups, normal and $CCl_4$-thiopental sod. intoxicated group. And the one group was treated intragastrically with the mixture of $CCl_4$-thiopental sod. 3 times per week for 3 weeks. The liver tissue and sera were used for the measurement of hydroxyproline (HYP), malonedialdehyde (MDA) and superoxide dismutase (SOD). Biochemical parameters such as aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total-bilirubin and blood urea nitrogen (BUN) were measured. Additionally, the expression of collagen ${\alpha}1$(III) and $\beta$-actin mRNA was observed by RTPCR. The histological change in liver tissue was also observed by Masson's trichrome and H&E staining. Correlation analysis was carried by Spearman's rho method. All biochemical parameters except total-bilirubin were significantly higher in the $CCl_4$-thiopental sod. treated group than that of the normal group (p < 0.01). In the $CCl_4$-thiopental sod. treated group, Hyp as a parameter of collagen synthesis (deposition) and MDA as a metabolite of lipid peroxidation, were significantly elevated by 1.98 and 2.11 times higher than that of the normal group (p < 0.001) respectively. The activity of SOD in the $CCl_4$-thiopental sod. treated group is decreased significantly by 44.8% (p < 0.001). And collagen ${\alpha}1$(III) mRNA was more expressed in the $CCl_4$-thiopental sod. treated group than that of the normal group. However, the expression of $\beta$-actin mRNA is showed similar in both of groups. A good correlation was observed between the content of hyp and MDA concentration (r = 0.70, n = 40) in the two groups. And the correlation between the levels of hyp and SOD (r = -0.71, n = 25) is also reliable. However, no correlation were observed between MDA concentration and SOD (r = -0.40, n = 25) in the two groups. Elevated levels of MDA in $CCl_4$-thiopental sod. treated rats indicated enhancement of lipid peroxidation, which is accompanied by a decrease in SOD activity. Moreover, we could confirm that the parameters of collagen synthesis (and deposition) is in good correlation with the metabolite of lipid peroxidation (MDA) and the lipid peroxidation antagonizing enzyme (SOD). Hence, we propose that (1) lipid peroxidation and collagen synthesis (and deposition) could be enhanced by intragastrically application of $CCl_4$-thiopental sod. during a short terms. And (2) the intoxication of $CCl_4$-thiopental sod. could be used for monitoring of lipid peroxidation and collagen synthesis (and deposition) for test of antioxidant and antifibrotic agent.

분리형 스페리칼 교량받침의 특징 및 교체실험

박성우(Park Sung-Woo),유승규(Liu Syung Kyu),최은수(Choi Eun-soo) 한국철도학회 2008 한국철도학회 학술발표대회논문집 Vol.- No.-

This study developed a new separated shperical bridge bearing that can be used for replacement of existing bridge bearings without crushing bearing-concrete. The separated spherical bearing have the upper and lower sole plate connected by bolts to the upper plate under bridge-girders and the lower plate on bearing-concrete. The targets of the separated spherical bearing are the maximum 3 ㎜ of up-lifting height during replacement and the maximum required time of 30 minutes. Four separated spherical bearings are manufactured and replaced the existing bearings of a railway bridge in service and the replacing tests was performed. The number of the tests is two and the target of maximum 3 ㎜ and 30 minutes was satisfied.

Analgesic effectiveness of nerve block in shoulder arthroscopy: comparison between interscalene, suprascapular and axillary nerve blocks.

Lee, Sang Mook,Park, Sang-Eun,Nam, Yong-Seok,Han, Seung-Ho,Lee, Kwang-Jin,Kwon, Min-Jeong,Ji, Jong-Hun,Choi, Syung-Kyun,Park, Jang-Su Springer International 2012 KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY Vol.20 No.12

<P>Postoperative pain in arthroscopic shoulder surgery cannot be easily controlled with analgesics and nerve blocks. This study shows the analgesic effect of interscalene block (ISB) and suprascapular nerve block and axillary nerve block (SSNB + ANB) in patients under patient controlled analgesia (PCA).</P>

한약재(자하거, 백출, 차전자) 추출물의 간경화 억제효능에 관한 연구

유병수,이종형,조성은,백승화,Yu, Byung-Soo,Lee, Jong-Hyung,Cho, Syung-Eun,Baek, Seung-Hwa 대한약학회 2007 약학회지 Vol.51 No.1

Hepatic cirrhosis is an important feature of chronic liver disease. Liver cirrhosis is characterized by hyperaccumulation of fibrous tissue components and is commonly observed in latter or terminal states of chronic hepatic disease. The antifibrotic effects on liver cirrhosis by oriental herbs extraction material were examined in bile duct ligated rats. Oriental herbs extraction (0.99 mg/kg rat weight/day) was administrated to cirrohotic rats for 4 weeks. Liver collagen content of bile duct ligated rats was significantly increased. And liver histology showed collagen fiber deposition was increased as well as the normal architecture was lost with large zone of necrosis being observed. Herbs extraction administrated rats showed significantly decreased liver collagen content, accumulation of collagen fiber in histological analysis, and biochemical markers of hepatic diseases. Those results demonstrate the usefulness of herbs extraction materials as an antifibrotic agent for liver cirrhosis.