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      • SCISCIESCOPUS

        Skermanella mucosa sp. nov., isolated from crude oil contaminated soil

        Subhash, Y.,Yoon, D. E.,Lee, S. S. Kluwer Academic Publishers [etc.] 2017 Antonie van Leeuwenhoek Vol.110 No.8

        <P>A novel Gram-stain negative, small rod-shaped bacterium (strain 8-14-6(T)) was isolated from hydrocarbon contaminated desert soil collected from Kuwait. Strain 8-14-6(T) grew at 5-37 A degrees C, pH 6.0-8.8 and 0-2% (w/v) of NaCl concentration. Casein, starch, Tween 20 and Tween 80 were hydrolyzed while urea, chitin, DNA and carboxymethyl-cellulose were not hydrolyzed by strain 8-14-6(T). The major cellular fatty acids were identified as C-18:1 omega 6c/C-18:1 omega 7c, C-16:0 and iso-C16:1I/C(14:0)3-OH. Strain 8-14-6(T) produced diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified phospholipids, two unidentified lipids and five unidentified amino lipids as polar lipids. Genomic G+C content was 73.5 mol %. 16S rRNA gene sequence comparisons indicated that strain 8-14-6(T) represents a member of the genus Skermanella within family Rhodospirillaceae of the class Alphaproteobacteria. Strain 8-14-6(T) has a sequence similarity of 98.9% with Skermanella rosea M1(T), 97.4% with Skermanella aerolata 5416T-32(T), 96.9% with Skermanella stibiiresistens SB22(T) and < 95.4% with the other two known species of the genus Skermanella. The DNA-DNA relatedness values between strain 8-14-6(T) and the type strains of the closely related species were clearly below the 70% threshold. From the combination of phenotypic and genotypic characteristics and distinct phylogenetic position, the strain is considered to represent a novel species of the genus Skermanella, for which the name Skermanella mucosa sp. nov. is proposed. The type strain is 8-14-6(T) (=KEMB 2255-438(T) =JCM 31590(T)).</P>

      • KCI등재
      • KCI등재

        SAFETY ASPECTS OF INTERMEDIATE HEAT TRANSPORT AND DECAY HEAT REMOVAL SYSTEMS OF SODIUM-COOLED FAST REACTORS

        SUBHASH CHANDER CHETAL 한국원자력학회 2015 Nuclear Engineering and Technology Vol.47 No.3

        Twenty sodium-cooled fast reactors (SFRs) have provided valuable experience in design, licensing, and operation. This paper summarizes the important safety criteria and safety guidelines of intermediate sodium systems, steam generators, decay heat removal systems and associated construction materials and in-service inspection. The safety criteria and guidelines provide a sufficient framework for design and licensing, in particular by new entrants in SFRs.

      • KCI등재

        Climate change, boundary increase and elongation of a pre-existing cline: A case study in Drosophila ananassae

        Subhash RAJPUROHIT,Ravi PARKASH,Shama SINGH,Seema RAMNIWAS 한국곤충학회 2008 Entomological Research Vol.38 No.4

        During the past two to three decades, Drosophila ananassae, a warm adapted tropical species, has invaded low to mid altitude localities in the western Himalayas. Due to its cold sensitivity, this species had never been recorded from higher latitudes as well as altitudes in India to the 1960s. A latitudinal cline in this desiccation-sensitive species corresponds with southern humid tropical localities rather than northern drier subtropical localities. An extension of its cline into lowland to midland montane localities has resulted due to global climatic change as well as local thermal effects through anthropogenic impact. However, D. ananassae populations at species borders are characterized by lower genetic variability for body melanization as well as for desiccation resistance. There is a lack of thermal plastic effects for body melanization, and the observed extended cline might represent evolutionary (genetic) response due to selection pressure imposed by drier habitats. A comparison of fecundity, hatchability and viability at three growth temperatures (17, 20 and 25°C) showed significant reduction in trait values at 17 °C in D. ananassae. Thus, its recent range expansion into northern montane localities might involve genetic effects on stress-related traits and plastic effects on life history traits. We suggest that D. ananassae could serve as an indicator species for analyzing range expansion under changing climatic conditions.

      • KCI등재

        On weighted Weyl spectrum, II

        Subhash Chander Arora,Preeti Dharmarha 대한수학회 2006 대한수학회보 Vol.43 No.4

        In this paper, we show that ifT is a hyponormal opera-tor on a non-separable Hilbert spaceH, then Re!0(T) !0(ReT),where !0(T) is the weighted Weyl spectrum of weight with @0 h := dim H. We also give some conditions under which theproduct of two-Weyl operators is-Weyl and its converse implica-tion holds, too. Finally, we show that the weighted Weyl spectrumof a hyponormal operator satises the spectral mapping theoremfor analytic functions under certain conditions.

      • KCI등재

        Performance Analysis of Disturbance Estimation Techniques for Robust Position Control of DC Motor

        Subhash S. Sankeshwari,Rajan H. Chille 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.2

        In this paper, the problem of high-performance robust position control of a DC motor based on the uncertainty and disturbance estimator (UDE) is presented. The UDE based sliding mode controller (SMC) is proposed which makes the system insensitive to the effect of Coulomb’s friction, parameter variations, and variable external load in DC motor. Fair comparison between UDE based SMC and existing classical technique is carried out by making PID as a robust controller by using Q-filter design. The different filter designs provide different estimates of nonlinearity, uncertainties, and external disturbances at a different resolution of position encoders. The proposed method guarantees robustness and ultimately eliminates the chattering phenomenon which is generally occurred in SMC.

      • KCI등재

        Solid Oxide Fuel Cells : Design, Materials, and Application

        Subhash C. Singhal 한국세라믹학회 2005 한국세라믹학회지 Vol.42 No.12

        The solid oxide fuel cell (SOFC) is an electrochemical device to convert chemical energy of a fuel into electricity at temperatures from about 600 to 1000oC. The SOFC offers certain advantages over lower temperature fuel cells, notably its ability to use CO as a fuel rather than being poisoned by it, and high grade exhaust heat for combined heat and power, or combined cycle gas turbine applications. This paper reviews the operating principle, materials for different cell and stack components, cell designs, and applications of SOFCs. Among all designs of solid oxide fuel cells (SOFCs), the most progress has been achieved with the tubular design. However, the electrical resistance of tubular SOFCs is high, and specific power output (W/cm2) and volumetric power density (W/cm3) low. Planar SOFCs, in contrast, are capable of achieving very high power densities.

      • Description of Comamonas sediminis sp. nov., isolated from lagoon sediments

        Subhash, Y.,Bang, John J.,You, Taek H.,Lee, Sang-Seob Society for General Microbiology 2016 International journal of systematic and evolutiona Vol.66 No.-

        <P>Strain S3(T) was isolated from lagoon sediments, and appeared as transparent colonies on agar plates, with cells staining Gram-negative. Catalase and oxidase were positive. S3(T) hydrolyzed starch, casein and tween-20, while urea, chitin, gelatin and tween-80 were not hydrolysed. C-18:1 omega 6C/C-18:1 omega 7C, C-16:1 omega 6C/C-16:1 omega 7c,C-17:0 cyclo and C-16:0 were the predominant fatty acids with minor amounts of C-10:0 3-OH, C-12:0, C-14:0 and C-16:0 2-OH. S3(T) contained diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), and phosphatidylethanolamine (PE) as major polar lipids with minor amounts of unidentified phospholipid (PL) and unidentified lipids (L1-2). Genomic DNA G+C content was 68.3 mol%. 16S rRNA gene sequence comparisons indicated that S3(T) represents a member of the genus Comamonas in family Comamonadaceae of the class Betaproteobacteria. S3(T) has a sequence similarity of 98.96% with Comamonas koreensis YH12(T), 97.93% with Comamonas guangdongensis CY01(T) and <96.97% with other members of the genus Comamonas. DNA DNA hybridization values between S3(T) and the type strains of the most closely related species were clearly below the 70% threshold. On the basis of phenotypic, genotypic and phylogenetic analysis, it is proposed that S3(T) represents a novel species of the genus Comamonas, for which the name Comamonas sediminis sp. nov. is proposed. The type strain is S3(T) (=KEMB 563-466(T) =JCM 31169(T)).</P>

      • Description of Oceanispirochaeta sediminicola gen. nov., sp. nov., an obligately anaerobic bacterium isolated from coastal marine sediments, and reclassification of Spirochaeta litoralis as Oceanispirochaeta litoralis comb. nov.

        Subhash, Y.,Lee, Sang-Seob Society for General Microbiology 2017 International journal of systematic and evolutiona Vol.67 No.9

        <P> An obligately anaerobic spirochaete (strain SY2<SUP>T</SUP>) was isolated from coastal marine sediments of Tongyeong-Si, South Korea. Strain SY2<SUP>T</SUP> was helical-shaped and Gram-stain-negative. Strain SY2<SUP>T</SUP> was able to grow at 10-40 °C (optima, 25-30 °C), pH 6.3-8.8 (optima, pH 7.0-8.0) and with 1-7% (optimum, 2-3 %) NaCl concentration. Strain SY2<SUP>T</SUP> was negative for catalase and oxidase activity. The major end-products of glucose fermentation were acetate, ethanol, hydrogen and carbon dioxide. C<SUB>14 : 0</SUB>, C<SUB>16 : 0</SUB>, iso-C<SUB>15 : 0</SUB>, iso-C<SUB>14 : 0</SUB> 3-OH, iso-C<SUB>15 : 1</SUB> H/C<SUB>13 : 0</SUB> 3-OH and iso-C<SUB>17 : 1</SUB>ω9c were predominant fatty acids (>5 %) with minor amounts (<5 %) of C<SUB>18 : 0</SUB>, iso-C<SUB>13 : 0</SUB>, iso-C<SUB>17 : 0</SUB>, iso-C<SUB>17 : 1</SUB>/anteiso-C<SUB>17 : 1</SUB> B and C<SUB>16 : 1</SUB>ω6c/C<SUB>16 : 1</SUB>ω7c. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were major polar lipids. The genomic DNA G+C content was 53.5 mol%. 16S rRNA gene sequence comparisons indicated that strain SY2<SUP>T</SUP> represents a member of the family Spirochaetaceae in the phylum Spirochaetes. Strain SY2<SUP>T</SUP> has a sequence similarity of 95.1% with Spirochaeta litoralis R1<SUP>T</SUP> and <90.1% with other members of the genus Spirochaeta. Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain SY2<SUP>T</SUP> as a representative of a novel genus and species in the family Spirochaetaceae, for which the name Oceanispirochaeta sediminicola gen. nov., sp. nov. is proposed. The type strain is SY2<SUP>T</SUP>(=KEMB 3001-381<SUP>T</SUP>=DSM 104770<SUP>T</SUP>=KCTC 15593<SUP>T</SUP>). Reclassification of Spirochaeta litoralis as Oceanispirochaeta litoralis comb. nov. is also proposed based on polyphasic taxonomic analyses. </P>

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