A comparative study on the recruitment of shoulder stabilizing muscles and types of exercises

João Carlos Comel,Rosane Maria Nery,Eduardo Lima Garcia,Claudete da Silva Bueno,Edinara de Oliveira Silveira,Mariéle Marchezan Zarantonello,Marco Antonio Stefani 한국운동재활학회 2018 JER Vol.14 No.2

Biocatalysis and Fermentation Technology : Lipid and Citric Acid Production by Wild Yeasts Grown in Glycerol

( Karla Silva Teixeira Souza ),( Rosane Freitas Schwan ),( Disney Ribeiro Dias ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.4

In this study, crude glycerol was used as a carbon source in the cultivation of wild yeasts, aiming at the production of microbial lipids and citric acid. Forty yeasts of different sources were tested concerning their growth in crude and commercial glycerol. Four yeasts (Lindnera saturnus UFLA CES-Y677, Yarrowia lipolytica UFLA CM-Y9.4, Rhodotorula glutinis NCYC 2439, and Cryptococcus curvatus NCYC 476) were then selected owing to their ability to grow in pure (OD600 2.133, 1.633, 2.055, and 2.049, respectively) and crude (OD600 2.354, 1.753, 2.316, and 2.281, respectively) glycerol (10%, 20%, and 30%). Y. lipolytica UFLA CM-Y9.4 was selected for its ability to maintain cell viability in concentrations of 30% of crude glycerol, and high glycerol intake (18.907 g/l). This yeast was submitted to lipid production in 30 g/l of crude glycerol, and therefore obtained 63.4% of microbial lipids. In the fatty acid profile, there was a predominance of stearic (C18:0) and palmitic (C16:0) acids in the concentrations of 87.64% and 74.67%, respectively. We also performed optimization of the parameters for the production of citric acid, which yielded a production of 0.19 g/l of citric acid in optimum conditions (38.4 g/l of crude glycerol, agitation of 184 rpm, and temperature of 30oC). Yarrowia lipolytica UFLA CM-Y9.4 presented good lipid production when in the concentration of 30 g/l of glycerol. These data may be used for production in large quantities for the application of industrial biodiesel.

Light Quality on the In Vitro Growth and Production of Pigments in the Genus Alternanthera

Andressa Reis,Alitcia Moraes Kleinowski,Fátima Rosane Schuquel Klein,Renata Trevizan Telles,Luciano do Amarante,Eugenia Jacira Bolacel Braga 한국작물학회 2015 Journal of crop science and biotechnology Vol.18 No.5

In tissue culture, several chemical and physiological factors can influence the production of secondary metabolites. The growth response and increased secondary metabolite production generated by a high irradiance environment, can be used to determine a favorable habitat for the growth and conservation of medicinal plants. Thus, the present study aimed to evaluate the influence of light quality on growth and production of secondary metabolites in Alternanthera sessilis (L.) R. Br. ex DC. (sessile joyweed), Alternanthera philoxeroides (Mart.) Grisebach (alligatorweed), Alternanthera tenella Colla (joyweed), and Alternanthera brasiliana (L.) Kuntze (Brazilian joyweed) plants cultured in vitro. The species were grown in MS medium, for 45 days in different light qualities (blue, white, and red). Growth parameters and biochemical analysis were performed at the end of the study. The results show that the red light allowed a higher accumulation of biomass in most species; red and white light were great inductors for the production of betacyanin and the blue light favored the enhancement of flavonoids. In this manner, the quality of light can affect the betacyanin, betaxanthin, and flavonoid biosynthesis as well as growth patterns in the related Alternanthera species. Based on the data presented, we suggest that specific genes can encode the pigment production according to the light quality which exhibit distinct activation patterns in different plant species.

The synthesis, thermal stability, crystal structure and spectroscopic study of La0.80Sr0.20MnO3 powder obtained by the modified Pechini’s method

Moisés R. Cesário,Daniel A. Macedo,Rosane M. P. B. Oliveira,Patrícia M. Pimentel,Roberto L. Moreira,Dulce M. A. Melo 한양대학교 세라믹연구소 2011 Journal of Ceramic Processing Research Vol.12 No.1

Strontium-doped lanthanum manganite (La1−xSrxMnO3) is a commonly used cathode in solid oxide fuel cells. In this study,La0.80Sr0.20MnO3 powder was prepared by the modified Pechini’s method using gelatin as the polymerizing agent. The polymeric resin obtained was characterized by thermogravimetric analysis and the powder calcined at 900 oC for 4 h has been characterized by X-ray diffraction, scanning electron microscopy and infrared spectroscopy. The La0.80Sr0.20MnO3 phase exhibited rhombohedral symmetry. The infrared reflectance spectrum was dominated by a conduction mechanism besides the signature of less pronounced phonon features, characteristics of the crystal lattice. The present results indicated that the gelatin has been an efficient directional element to be used in La0.80Sr0.20MnO3 synthesis, and it is a low cost material, non toxic and makes the polymeric precursor synthesis less complicated.

Sugarcane Bagasse Hydrolysis Using Yeast Cellulolytic Enzymes

( Angelica Cristina De Souza ),( Fernanda Paula Carvalho ),( Cristina Ferreira Silva E Batista ),( Rosane Freitas Schwan ),( Disney Ribeiro Dias ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.10

Ethanol fuel production from lignocellulosic biomass is emerging as one of the most important technologies for sustainable development. To use this biomass, it is necessary to circumvent the physical and chemical barriers presented by the cohesive combination of the main biomass components, which hinders the hydrolysis of cellulose and hemicellulose into fermentable sugars. This study evaluated the hydrolytic capacity of enzymes produced by yeasts, isolated from the soils of the Brazilian Cerrado biome (savannah) and the Amazon region, on sugarcane bagasse pre-treated with H2SO4. Among the 103 and 214 yeast isolates from the Minas Gerais Cerrado and the Amazon regions, 18 (17.47%) and 11 (5.14%) isolates, respectively, were cellulase-producing. Cryptococcus laurentii was prevalent and produced significant β- glucosidase levels, which were higher than the endo- and exoglucanase activities. In natura sugarcane bagasse was pre-treated with 2% H2SO4 for 30 min at 150oC. Subsequently, the obtained fibrous residue was subjected to hydrolysis using the Cryptococcus laurentii yeast enzyme extract for 72 h. This enzyme extract promoted the conversion of approximately 32% of the cellulose, of which 2.4% was glucose, after the enzymatic hydrolysis reaction, suggesting that C. laurentii is a good β-glucosidase producer. The results presented in this study highlight the importance of isolating microbial strains that produce enzymes of biotechnological interest, given their extensive application in biofuel production.

Derivation and Culture of Putative Parthenogenetic Embryonic Stem Cells in New Gelatin Substrates Modified with Galactomannan

Rafael R. Ruggeri,Adriana Bos-Mikich,Fabiana F. Bressan,Nataly M. Siqueira,Flávio Meirelles,Nilo Frantz,Yeda F. Watanabe,Rosane M. D. Soares 한국고분자학회 2014 Macromolecular Research Vol.22 No.10

Human embryonic stem cells (ESC) lines to be used for cell therapies must be created and maintainedunder strict conditions, excluding the use of undefined supplements. Two key steps in the creation of a new embryonicstem cell line are adherence to the substrate and derivation towards the formation of a primary colony. Thebovine parthenote embryo model was used to test different matrices of gelatin nanofibers and gelatin/galactomannanfilms to be used for ESC derivation and culturing. Gelatin/galactomannan films were made in two concentrations ofgalactomannan, 0.1 and 0.3%, in an aqueous solution of gelatin and tested for gel cytotoxicity using cumulus cells(CCs). CCs showed normal cell morphology, with no sign of lysis or degeneration in any of the matrices tested. Innercell masses of parthenote blastocysts (n=116) were placed onto the gel matrices for culture. There were three or fourrepeats for each matrix. Our results showed a good rate of inner cell mass (ICM) adherence on the gelatin/galactomannanfilms (41%-44%) and one derivative of the gel nanofiber (17% adherence to the substrate). These resultsencouraged us to try new gelatin formulations to increase the rates of derivation and cell proliferation under definedculture conditions to comply with good manufacturing practice directives for the potential therapeutic use of ESCs.

Purification and Biochemical Properties of a Glucose-Stimulated β-D-Glucosidase Produced by Humicola grisea var. thermoidea Grown on Sugarcane Bagasse

Cesar Vanderlei Nascimento,Flávio Henrique Moreira Souza,Douglas Chodi Masui,Francisco Assis Leone,Rosane Marina Peralta,João Atílio Jorge,Rosa Prazeres Melo Furriel 한국미생물학회 2010 The journal of microbiology Vol.48 No.1

The effect of several carbon sources on the production of mycelial-bound β-glucosidase by Humicola grisea var. thermoidea in submerged fermentation was investigated. Maximum production occurred when cellulose was present in the culture medium, but higher specific activities were achieved with cellobiose or sugarcane bagasse. Xylose or glucose (1%) in the reaction medium stimulated β-glucosidase activity by about 2-fold in crude extracts from mycelia grown in sugarcane bagasse. The enzyme was purified by ammonium sulfate precipitation, followed by Sephadex G-200 and DEAE-cellulose chromatography, showing a single band in PAGE and SDS-PAGE. The β-glucosidase had a carbohydrate content of 43% and showed apparent molecular masses of 57 and 60 kDa, as estimated by SDS-PAGE and gel filtration, respectively. The optimal pH and temperature were 6.0 and 50°C, respectively. The purified enzyme was thermostable up to 60 min in water at 55°C and showed half-lives of 7 and 14 min when incubated in the absence or presence of 50 mM glucose, respectively, at 60°C. The enzyme hydrolyzed p-nitrophenyl-β-D-glucopyranoside, p-nitrophenyl-β-Dgalactopyranoside,p-nitrophenyl-β-D-fucopyranoside, p-nitrophenyl-β-D-xylopyranoside, o-nitrophenyl-β-Dgalactopyranoside,lactose, and cellobiose. The best synthetic and natural substrates were p-nitrophenyl-β-Dfucopyranoside and cellobiose, respectively. Purified enzyme activity was stimulated up to 2-fold by glucose or xylose at concentrations from 25 to 200 mM. The addition of purified or crude β-glucosidase to a reaction medium containing Trichoderma reesei cellulases increased the saccharification of sugarcane bagasse by about 50%. These findings suggest that H. grisea var. thermoidea β-glucosidase has a potential for biotechnological applications in the bioconversion of lignocellulosic materials.

Periodontal parameters in orthodontically tractioned teeth: A systematic review and meta-analysis

Paola Marques de Mattos,Flavio Magno Gonçalves,Isabela Bittencourt Basso,Aline Xavier Ferraz,Bianca Simone Zeigelboim,José Stechman-Neto,Rosane Sampaio Santos,Cristiano Miranda de Araujo,Odilon Guariz 대한치과교정학회 2022 대한치과교정학회지 Vol.52 No.6

Objective: This systematic review aimed to evaluate periodontal parameters in orthodontically tractioned teeth compared with the respective non-tractioned contralateral teeth. Methods: Search strategies were developed for six electronic databases and gray literature. Random-effects meta-analyses were performed for the outcomes of interest. Furthermore, the certainty of the evidence was assessed using the GRADE (Grading of Recommendations Assessment, Development, and Evaluation) tool. Results: Overall, 2,082 articles were identified, of which 24 were selected for the qualitative synthesis. A significant difference was observed between the impacted and contralateral teeth (mean difference [MD] = 0.25; 95% confidence interval [CI] = 0.10–0.40; I2 = 0%) when the gingival index was evaluated. Additionally, impacted teeth showed a greater probing depth, with a significant mean difference between the groups (MD = 0.14; 95% CI = 0.07–0.20; I2 = 6%). Most studies had a low risk of bias; however, the certainty of the evidence was very low owing to the design of existing studies. Conclusions: The evidence in the literature indicated that tractioned teeth might show worsening of periodontal parameters related to the gingival index and probing depth; however, the evidence remains uncertain about this outcome. Furthermore, probing depth should be considered regarding its clinical significance because of the small effect size observed.

Evaluation of the Efficacy of Flaxseed Meal and Flaxseed Extract in Reducing Menopausal Symptoms

Márcia Constantino Colli,Adelar Bracht,Andréa Assunção Soares,Andréa Luisa de Oliveira,Cinthia Gandolfi Bôer,Cristina Giatti Marques de Souza,Rosane Marina Peralta 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.9

The objective of this study was to evaluate the efficacy of flaxseed meal and flaxseed extract in reducing climacteric symptoms of menopausal women. Ninety menopausal women were randomly distributed into three study groups: group I received 1 g per day of flaxseed extract containing at least 100 mg of secoisolariciresinol diglucoside (SDG), group II received 90 g per day of flaxseed meal containing at least 270 mg of SDG, and group III received 1 g per day of collagen (placebo group). Subjects were assessed for menopausal symptoms by the Kupperman index at the beginning and at the end of the 6 months of treatment. Subjects were also assessed for endometrial thickness and vaginal cytology. The Kupperman index values at the beginning and end of the treatments were analyzed using the paired t-test. Both the flaxseed extract (P=.007) and the flaxseed meal (P=.005) were effective in reducing the menopausal symptoms when compared with the placebo control (P=.082). Alternatively, the changes in Kupperman index were also computed and submitted to analysis of variance. In this case, no significant differences were found (P=.084) although the data indicate a decreasing tendency for the Kupperman index by both the flaxseed extract and the flaxseed meal groups. Neither the flaxseed extract nor the flaxseed meal exerted clinically important estrogenic effects on the vaginal epithelium or endometrium as revealed by the absence of changes in the blood levels of follicle stimulating hormone and estradiol, as well as in the endometrial thickness, and vaginal epithelial maturation value. No serious adverse events related to the treatments were reported. Although the results of the present study do not allow an unequivocal conclusion about the action of flaxseed on the menopausal symptoms, they suggest that it could be premature to conclude that no such action exists. Clearly the matter still deserves further experimental attention.

Effect of Babassu Mesocarp As a Food Supplement During Resistance Training

Maísa Carvalho Rezende Soares,Mayara Cristina Pinto Silva,Francisco de Assis da Silva Almeida-Junior,Johnny Ramos Nascimento,Flavia Raquel Fernandes Nascimento,Rosane Nassar Meireles Guerra 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.4

The population widely uses babassu mesocarp (Attalea speciosa) as food and medicine. This study evaluated the use of babassu mesocarp as a food supplement during resistance training (RT). Male Swiss mice, 60 days old (weight 35–40 g), were divided into four groups (n = 8): control, untreated and untrained; babassu (babassu aqueous extract [BAE]), treated orally with aqueous extract of babassu mesocarp (25 mg/kg), five times a week, for 8 weeks; training (RT), submitted to RT consisting of stair climbing with progressive loads; and resistance training treated with babassu aqueous extract (RTBAE): RT and treatment with BAE. After 8 weeks, we analyzed the biochemistry of serum, the immunological, and histological parameters. The RT group showed maximum strength after the second week. A reduction in body weight, retroperitoneal and interstitial fat deposits, and activated helper T lymphocytes (TCD4+ CD69+) occurred in RT and RTBAE groups. The RTBAE group showed increased levels of aspartate aminotransferase, alanine aminotransferase, and macrophage and helper T lymphocyte count, whereas a reduction occurred in triglyceride levels and the total number of lymphocytes. Supplementation with BAE always reduced cholesterol and the population of activated macrophages but increased activated B lymphocytes and interleukin-6 levels. The combination of supplementation and RT resulted in a decreased production of tumor necrosis factor-α. We propose the use of babassu mesocarp as a food supplement during exercise because of its immunomodulatory effect on lymphocyte and macrophage populations and cytokine production. The additional impact on the control of cholesterol and triglyceride levels suggests its use, particularly for the treatment of dyslipidemias.