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An On-Line Real-Time SPC Scheme and Its Performance
Nishina, Ken The Korean Society for Quality Management 2001 The Asian Journal on Quality Vol.2 No.1
This paper considers a recent environment in the manufacturing process in which data in large amounts can be obtained on-line in real-time. Under this environment an on-line real-time Statistical Process Control (SPC) scheme equipped with detection of a process change, change-point estimation, and recognition of the change pattern is proposed. The proposed SPC scheme is composed of a Cusum chart, filtering methods and Akaike Information Criterion (AIC). We examine the performance of this scheme by Monte Carlo simulation and show its usefulness.
An On-Line Real-Time SPC Scheme and Its Performance
Ken, Nishina 한국품질경영학회 2001 The Asian Journal on Quality Vol.2 No.1
This paper considers a recent environment in the manufacturing process in which data in large amounts can be obtained on-line in real-time. Under this environment an on-line real-time Statistical Process Control (SPC) scheme equipped with detection of a process change, change-point estimation, and recognition of the change pattern is proposed. The proposed SPC scheme is composed of a Cusum chart, filtering methods and Akaike Information Criterion (AIC). We examine the performance of this scheme by Monte Carlo simulation and show its usefulness.
Kim, Y.,Nishina, K.,Chae, N.,Park, S. J.,Yoon, Y. J.,Lee, B. Y. Copernicus GmbH 2014 Biogeosciences Vol.11 No.19
<P><p><strong>Abstract.</strong> The tundra ecosystem is quite vulnerable to drastic climate change in the Arctic, and the quantification of carbon dynamics is of significant importance regarding thawing permafrost, changes to the snow-covered period and snow and shrub community extent, and the decline of sea ice in the Arctic. Here, CO<sub>2</sub> efflux measurements using a manual chamber system within a 40 m × 40 m (5 m interval; 81 total points) plot were conducted within dominant tundra vegetation on the Seward Peninsula of Alaska, during the growing seasons of 2011 and 2012, for the assessment of driving parameters of CO<sub>2</sub> efflux. We applied a hierarchical Bayesian (HB) model - a function of soil temperature, soil moisture, vegetation type, and thaw depth - to quantify the effects of environmental factors on CO<sub>2</sub> efflux and to estimate growing season CO<sub>2</sub> emissions. Our results showed that average CO<sub>2</sub> efflux in 2011 was 1.4 times higher than in 2012, resulting from the distinct difference in soil moisture between the 2 years. Tussock-dominated CO<sub>2</sub> efflux is 1.4 to 2.3 times higher than those measured in lichen and moss communities, revealing tussock as a significant CO<sub>2</sub> source in the Arctic, with a wide area distribution on the circumpolar scale. CO<sub>2</sub> efflux followed soil temperature nearly exponentially from both the observed data and the posterior medians of the HB model. This reveals that soil temperature regulates the seasonal variation of CO<sub>2</sub> efflux and that soil moisture contributes to the interannual variation of CO<sub>2</sub> efflux for the two growing seasons in question. Obvious changes in soil moisture during the growing seasons of 2011 and 2012 resulted in an explicit difference between CO<sub>2</sub> effluxes - 742 and 539 g CO<sub>2</sub> m<sup>−2</sup> period<sup>−1</sup> for 2011 and 2012, respectively, suggesting the 2012 CO<sub>2</sub> emission rate was reduced to 27% (95% credible interval: 17-36%) of the 2011 emission, due to higher soil moisture from severe rain. The estimated growing season CO<sub>2</sub> emission rate ranged from 0.86 Mg CO<sub>2</sub> in 2012 to 1.20 Mg CO<sub>2</sub> in 2011 within a 40 m × 40 m plot, corresponding to 86 and 80% of annual CO<sub>2</sub> emission rates within the western Alaska tundra ecosystem, estimated from the temperature dependence of CO<sub>2</sub> efflux. Therefore, this HB model can be readily applied to observed CO<sub>2</sub> efflux, as it demands only four environmental factors and can also be effective for quantitatively assessing the driving parameters of CO<sub>2</sub> efflux.</p> </P>
Takashi Dojima,Takuya Nishina,Tatsuya Kato,Hiroshi Ueda,Enoch Y. Park 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.3
Silkworms are useful bioreactors for heterologous protein expression when used in conjunction with the Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid system. However, purification from silkworm hemolymph is difficult since it contains various kinds of proteins. In this study, we investigated an effective single-step method for the purification of affinity-tagged single-chain antibody variable region fragment (scFv) from silkworm larval hemolymph. A 5-fold higher expression level was obtained when scFv was fused with the His tag than when it was fused with the Strep II or GST tags. However, the His tag was inadequate for single-step purification since it led to the nonspecific binding of contaminants. The purification recoveries of GST-, Strep II-, and His-tagged scFvs were 91.8%, 43.7%, and 27.2%, respectively. The specific amount of single-step purified GST-tagged scFv was 2.2~2.7 fold higher than the amounts of the His- and Strep II-tagged constructs. The purities of Strep II- and GST-tagged scFvs in the eluent were 98.4% and 83.0%, respectively. Thus, both the short peptide Strep II and GST protein are suitable fusion tags for the affinity purification of proteins from silkworm larvae Silkworms are useful bioreactors for heterologous protein expression when used in conjunction with the Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid system. However, purification from silkworm hemolymph is difficult since it contains various kinds of proteins. In this study, we investigated an effective single-step method for the purification of affinity-tagged single-chain antibody variable region fragment (scFv) from silkworm larval hemolymph. A 5-fold higher expression level was obtained when scFv was fused with the His tag than when it was fused with the Strep II or GST tags. However, the His tag was inadequate for single-step purification since it led to the nonspecific binding of contaminants. The purification recoveries of GST-, Strep II-, and His-tagged scFvs were 91.8%, 43.7%, and 27.2%, respectively. The specific amount of single-step purified GST-tagged scFv was 2.2~2.7 fold higher than the amounts of the His- and Strep II-tagged constructs. The purities of Strep II- and GST-tagged scFvs in the eluent were 98.4% and 83.0%, respectively. Thus, both the short peptide Strep II and GST protein are suitable fusion tags for the affinity purification of proteins from silkworm larvae