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Muwang Wei,Jianhe Xie,Weicai Liu 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.41 No.2
To meet the demands of the sustainable development of construction, the combination of precast structures and recycled aggregate concrete made from construction and demolition waste is being promoted as a promising green construction technology. In this study, a new prefabricated member, a buckling-restrained steel plate shear wall with a cover plate made of recycled aggregate concrete (PBRW), was developed and experimentally studied. Eight specimens were tested to study the effect of the cover plate with different recycled aggregate substitution ratios and various bolt arrangements on the seismic behavior of this shear wall system. Based on the high-order buckling in the inner steel plate, a theoretical method was proposed to predict the shear resistance of PBRWs. The test results indicated that the PBRWs exhibited high shear strength, an adequate initial stiffness, a favorable energy absorption capacity, and a stable hysteresis curve. A full replacement of recycled aggregate with natural aggregate had almost no adverse impact on the seismic behavior of the PBRWs. The wall with an insufficient number of bolts (bolt arrangement of 3×2) imposed weaker lateral constraints on the inner plate, resulting in a reduction in the seismic behavior.
Studies on the Hereditary Properties of SSR Marker in Silkworm (Bombyx mori L.)
Li Muwang,Li Minghui,Miao Xuexia,Lu Cheng,Huang Yongping Korean Society of Sericultural Science 2005 International Journal of Industrial Entomology Vol.11 No.1
Two BC1 group, c108 $\times$ (p50 $\times$ c108) and p50 $\times$ (p50 $\times$ c108), one group of F$_{2}$ progeny, (p50 $\times$ c108) F$_{2}$ ,and 3 SSR markers, F10539, FlO626 and FlO618 were used to test the hereditary properties of SSR markers in silkworm. FI0539, FlO626 were proved to be linkage, and FlO618 was proved to be independent to those two markers. According to Mendel's law, the recombinant value between F10539, FlO626 was calculated in all of these groups, and they were 8.55$\%$ (c108BC1), 8.02$\%$ (p50BC1) and 7.81 $\%$ (F$_{2}$) respectively. There was dominant difference among the crossing-over value using paired-samples tests by SPSS 10.0 software. This research proved that SSR markers were co-dominant in B. mori too, and F 2 progeny could be used to construct SSR linkage map although B. mori lacked of crossing over in females.
PCR-Based Detection of Densovirus Infection in Silkworm (Bombyx mori L.)
Hou Chengxiang,Li Muwang,Gui Zhongzheng,Xu Anying,Guo Xijie Korean Society of Sericultural Science 2005 International Journal of Industrial Entomology Vol.11 No.2
Two pairs of DNA primers were designed for the detection of the Zhenjiang (China) strain of Bombyx mori densonucleosis virus (BmDNV-Z). These primers were designed from the nucleotide sequence of major structural protein gene (putative VD1-ORF2). PCR amplification was attempted from different issues (including silk gland, blood, skin and midgut) and feces of the silkworm which infected wit BmDNV-Z were amplified by PCR. Both of the primers gave expected size of in the DNA bands from midgut and feces, but not in the DNA of silk gland, blood and skin. The two bands were sequenced, and their sequence were same as the sequence designed for. BmDNV-Z could be successfully detected in single silkworm after it was infected for 12 hrs, and could not be detected before 9 hrs after infected.
Screening of RAPD Markers for Fluoride Resistance in Bombyx mori L.
Chen, Keping,Yao, Qin,Li, Muwang,Wang, ong Korean Society of Sericultural Science 2003 International Journal of Industrial Entomology Vol.7 No.1
NF733xin, the near allele line was obtained by means of crossing and backcrossing the silkworm race T6, which contained fluoride resistance major gene, to race 733xin, which was highly susceptible to fluoride toxicity. Two hundred RAPD random primers were used in the RAPD analysis of these 3 strains. Two molecular markers, OPB-08850 and OPB-10917, were obtained. OPB-10917 was used to detect the backcross generations. It was found that all the fluoride resistant individuals in each backcross generation had the same special band. These results proved that this marker was reliable.
Xu, Anying,Lin, Changqi,Hou, Chengxiang,Zhang, Yuehua,Li, Muwang,Sun, Pingjiang Korean Society of Sericultural Science 2006 International Journal of Industrial Entomology Vol.13 No.1
The major dominant fluoride-endurance (Dfe) gene was introduced into the commercial varieties by crossing and pedigree selection to breed silkworm races that could normally develop in the area that polluted by fluoride. After backcrossed for two generations, the Dfe gene was made homozygous, and individuals with good economic characters were selected to generate next generation. After 8 generations of selection, their characters became stable, and the silkworm variety which is resistant to fluoride, Huayuan${\times}$Dongsheng, for spring rearing were bred.
Li Tao,Qin Sheng,Sun Xia,Zhang Kai-xiang,Ding Xin-yi,Wang Xue-yang,Li Muwang 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.3
Baculoviruses are enveloped rod-shaped viruses that with circular double-stranded and large DNA genome. Baculoviruses successfully invade by using the host factors, especially at the process of establishing early infection. In this study, we investigated the different resistant strains of silkworm in response to AcMNPV early infection by RNA-sequencing. Our data revealed that the genes involved in innate immunity and ribosomal proteins were suppressed and cytoskeleton were induced in susceptible strain p50, thus facilitating the viral replication. However, in resistant strain C108, the genes participated in endocytosis and RNA transport were down-regulated, while up-regulation of ribosomal proteins and energy metabolic to inhibit the infection of the virus. These data provide a new sight of the initial host transcriptional responses to viral infection in silkworm.
Metabolomics reveals abundant flavonoids in edible insect Antheraea pernyi
Fu Xin,Chai Chun-Li,Li Yu-Ping,Li Peng,Luo Shi-Hong,Li Qun,Li Muwang,Liu Yan-Qun 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3
The natural flavonoids in foods of plant origin have been well-characterized due to their beneficial biological properties. However, the information regarding the flavonoid compounds in edible insects remains severely limited. In the present study, we used a metabolomics approach to identify the flavonoid compounds in the Chinese oak silkworm, Antheraea pernyi Guérin-Méneville (Lepidoptera: Saturniidae), an traditional edible insect. Our study identified over 200 flavonoid metabolites in the larval midgut of A. pernyi with LC-ESI-MS/MS system. These flavonoid metabolites come from eight subclasses, including flavones (1 0 3), flavonols (34), flavonoids (28), flavanones (20), polyphenols (19), isoflavones (9), anthocyanins (9), and proanthocyanidins (4). The relative content of the flavones is the most abundant, with a value of 36.74% of the total. The top five flavonoid components in A. pernyi are hyperoside, isoquercitroside, tricin 7-O-hexoside, hesperetin 5-O-glucoside and protocatechuic acid, accounting for 51.17% of the total flavonoids. Hyperoside is the most abundant flavonoid compound (18.07% of the total) in A. pernyi. Our findings indicated targeted metabolomics is a useful approach to identify flavonoids in edible insects which contain abundant flavonoids than we already knew.