http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Kulatunga, D.C.M.,Dananjaya, S.H.S.,Nikapitiya, Chamilani,Godahewa, G.I.,Cho, Jongki,Kim, Cheol-Hee,Lee, Jehee,De Zoysa, Mahanama Elsevier 2019 Fish & Shellfish Immunology Vol.84 No.-
<P><B>Abstract</B></P> <P>Proteins with dithiol-disulfide oxidoreductase catalytic domain are well known for their capacity in the cellular redox homeostasis. In this study, we characterized the zebrafish thioredoxin domain containing 12 (Zf<I>txndc12</I>) gene, analyzed the transcriptional responses and studied the functional properties of its recombinant protein. Full-length cDNA of Zf<I>txndc12</I> consists 519 bp coding region encoding 172 amino acids (AA) including the signal peptide. Highly consensus active motif (<SUP>65</SUP>WCGAC<SUP>69</SUP>) and probable ER retrieval motif (<SUP>169</SUP>GDEL<SUP>172</SUP>) were identified. Ubiquitous expression of Zf<I>txndc1</I>2 mRNA was observed from one cell to juvenile stage as well as different organs of adult zebrafish. Moreover, whole mount <I>in situ</I> hybridization (WISH) results showed a higher expression of Zf<I>txndc12</I> in primordial gills, central nerves system and eye. The tissue specific expression analysis (by qRT-PCR) also showed the highest expression in gills followed by brain in adult zebrafish. In larvae, up-regulated Zf<I>txndc1</I>2 mRNA expression upon exposure to H<SUB>2</SUB>O<SUB>2,</SUB> <I>Edwardsiella tarda</I> and <I>Saprolegnia parasitica</I> suggests that it may involve in both stress and immune responses. Moreover, transcriptional expression of Zf<I>txndc12</I> was up-regulated upon <I>Streptococcus iniae</I> challenge in gills of adult zebrafish<I>.</I> The recombinant ZfTxndc12 (rZfTxndc12) was overexpressed, purified and tested for its biological activities. Results revealed that rZfTxndc12 is able to reduce the DNA damage and detoxify the H<SUB>2</SUB>O<SUB>2</SUB> toxicity in concentration dependent manner. Overall results suggest that Zf<I>txndc12</I> is important antioxidant and immune functional member of the host defense system in zebrafish.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Txndc12 is important for oxidative stress defense and redox regulation. </LI> <LI> Constitutive expression of Zftxndc12 is ubiquitous in embryonic developmental stages and adult zebrafish. </LI> <LI> Immune challenge and oxidative stress induce the Zftxndc12 transcription in zebrafish larvae and adult's gills. </LI> <LI> >Recombinant Zftxndc12 shows <I>in vitro</I> ROS scavenging and cytoprotective activity. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Neuronal over-expression of human Alzheimer's disease related genes in canines
Chanuka Kulatunga,Dong Eon Kim,JI Hye Lee,Kuk Bin Ji,Eun Ji Lee,Kyeong Yeob Kim,Beom Sik Kim,Kyu Hyun Kim,Ryeong Eun Kim,Yoon Seok Nam,Min Kyu Kim 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11
The early-onset familial Alzheimer's disease (EOFAD/ FAD), the less common type of Alzheimer's disease (AD) currently affects a vast number of individuals worldwide. This type is being inherited as an autosomal dominant fashion. Missense mutations on Amyloid precursor protein (APP) and Presenilins 1 and 2 (PSEN1 & PSEN2) are known as major genetic factors in FAD. Conversely, missense mutations on microtubule-associated protein tau (MAPT) are also thought to involve. Up to date, several triple-transgenic animal models with muted forms of the human APP, PSENs and MAPT have been reported. Compared to other animals, canines are more emotional and their disease signs can be easily diagnosed. This attempt was to develop a triple transgenic canine model for the AD. We have obtained the coding sequences of APP, PSEN1 and MAPT from Dana-Farber/Harvard Cancer Center DNA resource core at HMS and incorporated several common AD mutations. The transgenic construct is composed of hNSE (ENO2) promoter-driven three AD genes fused together with modified 2A sequences. It was transfected into the canine fetal fibroblasts which were then used to perform somatic cell nuclear transfer (SCNT). The viable transgenic embryos were obtained after in vitro culture and the GFP was detected. In this study, we have successfully produced viable triple transgenic canine cloned embryos using SCNT technique. These transgenic canine embryos will be further developed into canines with FAD. The transgenic canines will be a good candidate in the AD research field.
Chi Sun Yun,Dong Eon Kim,Chanuka Kulatunga,Eun Ji Lee,Ji Hye Lee,Eun Young Kim,Ju Lan Chun,Min Kyu Kim 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06
The endemic species of sika deer in Northeast Asia is endangered animals in South Korea. In the 1970s, some subspecies (C.n.taiouanus and C.n.yesoensis) were introduced from other countries for restoration, and were not the endemic species (C.n.hortulorum) of Northeast Asia. However, there has not been a sufficient study about endemic and reintroduced sika deer. The purpose of this study was to identify the genetic diversity and relationship of South Korean sika deer compared to those in other countries at Northeast Asia. Total 153 DNA samples (69 South Korean, 38 Chinese and 29 North Korean samples of captive bred, and 17 Russian of wild) were used in the analysis. The number of alleles per locus varied from 2~14 with a mean of 6. The expected heterozygosity and observed heterozygosity values were 0.3863 and 0.2740, respectively. The mean polymorphism information content value was 0.3528, ranging from 0.026~0.826. The genetic distance analysis showed genetic difference between South Korean and other populations, and Russian and North Korean populations were genetically similar. The maximum value of ΔK was observed when K was set to 2. When K=2, the genetic structure analysis revealed hybridization among four populations. Based on the information from structure results, we distinguished individuals with dominant genetic composition of a particular subspecies (>0.90), and the result showed relatively ‘pure’ individuals in South Korean population formed a distinct cluster that contradict other countries. As a result, all the sika deer populations in the four countries were genetically vulnerable. In addition, it is confirmed that relatively ‘pure’ individuals in South Korea were showed genetically different characteristics compared to other countries. However, the rate of hybridization was high in all populations of four countries, and it was also difficult to grasp the hybridization with other deer subspecies or other species. Moreover, STRUCTURE analysis results depending on the number and type of markers used for analysis. Therefore, additional samples and markers need to be acquired and analyzed.
( Sangyeop Shin ),( D. C. M. Kulatunga ),( S. H. S. Dananjaya ),( Chamilani Nikapitiya ),( Jehee Lee ),( Mahanama De Zoysa ) 한국균학회 2017 Mycobiology Vol.45 No.4
Saprolegniasis is one of the most devastating oomycete diseases in freshwater fish which is caused by species in the genus Saprolegnia including Saprolegnia parasitica. In this study, we isolated the strain of S. parasitica from diseased rainbow trout in Korea. Morphological and molecular based identification confirmed that isolated oomycete belongs to the member of S. parasitica, supported by its typical features including cotton-like mycelium, zoospores and phylogenetic analysis with internal transcribed spacer region. Pathogenicity of isolated S. parasitica was developed in embryo, juvenile, and adult zebrafish as a disease model. Host-pathogen interaction in adult zebrafish was investigated at transcriptional level. Upon infection with S. parasitica, pathogen/antigen recognition and signaling (TLR2, TLR4b, TLR5b, NOD1, and major histocompatibility complex class I), pro/anti-inflammatory cytokines (interleukin [IL]-1β, tumor necrosis factor α, IL-6, IL-8, interferon γ, IL-12, and IL-10), matrix metalloproteinase (MMP9 and MMP13), cell surface molecules (CD8<sup>+</sup> and CD4<sup>+</sup>) and antioxidant enzymes (superoxide dismutase, catalase) related genes were differentially modulated at 3- and 12-hr post infection. As an anti-Saprolegnia agent, plant based lawsone was applied to investigate on the susceptibility of S. parasitica showing the minimum inhibitory concentration and percentage inhibition of radial growth as 200 μg/mL and 31.8%, respectively. Moreover, natural lawsone changed the membrane permeability of S. parasitica mycelium and caused irreversible damage and disintegration to the cellular membranes of S. parasitica. Transcriptional responses of the genes of S. parasitica mycelium exposed to lawsone were altered, indicating that lawsone could be a potential anti-S. parasitica agent for controlling S. parasitica infection.
L-carnitine supplementation improves the cryosurvival and subsequent development of bovine embryos
Kyeong Yeob Kim,Youn Bae Park,Byeong Ho Kim,Jin Hee Lee,Ji Hye Lee,Chanuka Kulatunga,Dong Eon Kim,Kyu Hyun Kim,Ryeong Eun Kim,Yoon Seok Nam,Min Kyu Kim 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11
Cryopreservation of bovine embryos is used to efficiently implant surrogate mothers. It has been widely accepted that high lipid content in the oocyte interrupts its survival during freeze-thaw cycles. Serum component in the culture medium is thought to increase the embryo`s lipid contents. Conversely, L-carnitine stimulates lipid metabolism by transporting long chain fatty acids into the mitochondria. Objective of this study was to analyze the effect of L-carnitine supplementation in IVM medium and defined IVC medium on the development, lipid contents and the cryosurvival of bovine IVF embryos. 0.0, 1.5, 3.0 and 6.0 mM L-carnitine was supplemented in IVM medium, respectively (IVM-LC 0.0, LC 1.5, LC 3.0 and LC 6.0). Development rate from the 2cell to the morula stages was higher in IVM-LC 3.0 groups than those of IVM-LC 6.0 (p<0.05). But there were no significant differences among the other groups in the blastocyst rates and lipid content results. When 0.0, 1.5, 3.0 and 6.0 mM L-carnitine were supplemented in IVC medium (IVC-LC 0.0, LC 1.5, LC 3.0 and LC 6.0), development competence was not significantly different between those embryos. Lipid contents of embryos treated L-carnitine (IVC-LC 1.5, 3.0 and 6.0) were significantly lower than embryos of non-treated group. L-carnitine was supplemented 0.0, 1.5, 3.0, 6.0 mM during IVM and 3.0 mM during IVC (LC 0.0 - 3.0, LC 1.5 – 3.0, LC 3.0 – 3.0, LC 6.0 – 3.0) and cryosurvival of blastocysts confirmed after freezing-thawing. There were no significant differences on development, but LC 3.0 – 3.0 was significantly lower lipid contents than other groups. And LC 3.0 – 3.0 had better survival rates and hatched rates of blastocysts than LC 0.0 – 0.0. In conclusion, supplementation of L-carnitine in defined IVC medium decreases lipid contents. And L-carnitine supplementation improves cryosurvival and developmental ability of bovine IVF embryos.