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General applications of modified Stokes expression for modeling and scale-up of expanded beds
Keun Garp Ryu,Yoon Mi Lee,권오열 한국화학공학회 2007 Korean Journal of Chemical Engineering Vol.24 No.2
The modeling of the behavior of expanded beds using Richardson-Zaki correlation in combination with the modified Stokes expression developed by Chae et al. [1] was successfully applied to the cases of small expanded beds (1 cm in column diameter) containing two different polydisperse particles used for ion-exchange operations, Chelating excellose (70-210 μm in diameter, 1.21 g/cm3 in density) and Streamline DEAE (100-300 μm in diameter, 1.20 g/cm3 in density). Empirical parameters determined for the modeling of the expansion of these small-beds (1 cm diameter) were found to have values similar to those for the case of larger expanded beds (2.5 cm in diameter) containing the same particles. This indicates that the modeling method can be utilized for the scale-up purposes of the expanded beds with various feed solutions.
( Keun Garp Ryu ),( Jun Sang Bae ),( Kyu Bum Kwack ),( O Yul Kwon ),( Sun Ho Park ) 한국미생물 · 생명공학회 2002 Journal of microbiology and biotechnology Vol.12 No.3
Lysis of erythrocytes isolated from human, rabbit, and mouse blood samples was investigated with the culture supernatant of Xenorhabdus nematophilus in a primary form. Prior to use, the culture supernatant of the bacteria was concentrated and the concentrate was dialyzed against Tris-HCl buffer (10 mM, pH 8.1) by ultrafiltration using PM-5 membrane with a molecular weight cut-off of 5,000. At 30℃, the supernatant exhibited no lytic activity towards three types of erythrocytes. However, at 4℃, the supernatant showed selective lytic activity towards rabbit erythrocytes within 90 min, yet did not lyze human or mouse erythrocytes. Microscopic examination clearly revealed that most of the rabbit erythrocytes had been turned into ghost forms.
Effects of Surfactants on the Enzymatic Blaching of Kraft Pulp by Xylanase
Ryu, Keun-Garp,Kim, Young-Gon The Korean Society for Biotechnology and Bioengine 1997 Biotechnology and Bioprocess Engineering Vol.2 No.2
A xylanase was purified from a commercial crude xylanase, Pulpzyme HC, and used for the bleaching of kraft pulp in the absence or in the presence of nonionic surfactants, Tween 20, Tween 80, and Igepal C930. The purified xylanase has a molecular weight of 23,500 as determined by a reducing SDS-PAGE. Tween 20 was most effective to enhance the efficiency of the enzymatic bleaching of kraft pulp by xylanase.
Ryu, Keun-Garp,Bae, Jun-Sang,Yu, Yeon-Su,Park, Sun-Ho The Korean Society for Biotechnology and Bioengine 2000 Biotechnology and Bioprocess Engineering Vol.5 No.2
Entomopathogenic nematodes are being used for insect control. We purified a toxin secreted by the insect-pathogenic bacterium, Xenorhadbus nematophilus, which lives in the gut of entomopathogenic nematodes. Culture broth of X. nematophilus was separated by centrifugation and concentrated by ultration. The concentrated culture broth was applied to a DEAE Sephadex A-50 column, and proteins were eluted stepwise with increasing concentrations of KCI. Fractions column. The molecty weight of purified toxin was39 kDa on SDS-PAGE, and Fourier tranformed infrared (FTIR) spectroscopy indicated that this toxin could be a new protein exhiting the charactristics of C=O stretching peak near 1650cm-1.
Horseradish Peroxidase 효소촉매에 의한 난연성 페놀고분자의 합성
유근갑 ( Keun Garp Ryu ),박한솔 ( Han Sol Park ),박정희 ( Jung Hee Park ),이학성 ( Hak Sung Lee ) 한국화학공학회 2013 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.51 No.1
The optimum synthetic conditions of poly(p-phenylphenol) by horseradish peroxidase in dioxane:water (80:20 v/v) mixtures were studied. The stability against thermal degradation and structural properties of the synthesized phenolic resins were investigated by thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC), respectively. The synthetic yield of poly(p-phenylphenol) increased upon the increase of the amount of enzyme up to 0.25 mg HRP/mL, then leveled off for further increase of the enzyme usage. When sodium acetate (100 mM, pH 4~6) and sodium phosphate (100 mM, pH 7~9) were used as the buffering salts for the aqueous component (20% v/v), the synthetic yield of the resin increased at higher pH of the aqueous buffer. But when the pHs of the aqueous buffer were 6 and 9, the synthetic yield strongly depended on the types of the buffering salts; if sodium phosphate was used instead of sodium acetate at pH 6, the yield decreased by about 15% and if sodium bicarbonate was used instead of sodium phosphate, the yield decreased by almost 20%. When the pH range of the aqueous buffer was from 4 to 7, the addition of a radical mediator, 2,2``-azinobis (3-ethylbenzothiazoline-6-sulfonate) (ABTS), up to 2 mM improved the synthetic yield of the resin by about 10%. TGA experiments revealed that the thermal stability of the resin synthesized in dioxane:water (100 mM sodium phosphate, pH 9) (80:20 v/v) was high having the char yield of 47% upon the heating at 800℃. DCS results showed that the structures of the polymers synthesized in acidic aqueous buffers were different from those of the polymers synthesized in the basic aqueous buffers. However, all the synthesized resins were found to have the property of the thermosetting resins.