한국인에서의 CYP2C19m₁과 CYP2C19m₂돌연변이 유전자형의 다형성

권준택,김형기 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.1

The S-mephenytoin 4 -hydroxylase (CYP2C19) metabolizes a number of clinically used drugs and shows a marked interethnic difference in the incidence of the poor metabolizer (PM). In the present study, we genotyped 215 healthy unrelated Koreans (64 females, aged 20-41 yr, residing in Chungcheong province) for functionally defective alleles, CYP2C19m1 and CYP2C19m2 Detection of the normal (CYP2C19wt) and defective alleles was performed by polymerase chain reaction/restriction enzyme analysis. The genomic DNA was isolated from peripheral blood. The allelic frequencies of the wild-type (CYP2C19wt) and CYP2C19m1 were 72.6% and 27.4%, and the wild-type (CYP2C19wt) and CYP2C19m2 were 85.1% and 14.9%, respectively. For each CYP2C19m1 and CYP2C19m2 the observed numbers of three genotypes were similar to those calculated in accordance with the Hardy-Weinberg equation. The frequencies of homozygotes for CYP2C19m1, CyP2C19m2 and compound heterozygotes were 9.7%, 2.8% and 4.2%, respectively. The mutants of CYP2C19 were identified in 36 subjects (16.7%). These results suggest that frequency of mutants of CYP2C19 in Koreans resembled the Orientals rather than Caucasians.

골프클럽 구매 결정과정에 미치는 영향에 관한 연구

임준택 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.4

The purpose of this study is to identify what motivates people to purchase golf 치ub. Candidates used in this study were people who have valid membership card in driving ranges located in seoul vicinity. Purpose Sampling Method was used to 250 students to conduct the case study but only 204 students results(8l%) were applied for final analysis. In order to identify the people's motivation, special survey drawn up by the expert was used and executed the result by way of t-Test and One Way Anova which showed following results; First, Satisfactory level of purchased product was different according to consumer's individual characteristic such as one's job, golf career and a primary factor of purchasing of golf club, Satisfactory level was high in the group having golf career more than 12 years and in the group considering the price of the product mainly, However, satisfactory level was low in the group having career less than 3 years and in the group considering product's reliability and consistency. Second, Reliability on mass media to purchase product was different according to consumer's individual characteristic. Reliability on mass media was high in the group having career more than 12 years or having a liberal profession, And reliability in male group was higher than female group. However, reliability was Low in female group and in the group having career less than 3 years. Third, Practical sense used to purchase product was different according to consumer's individual characteristic such as one's gender and family income, Practical sense of purchasing product in male group was higher than that of female, And the sense was high in the group purchased product more than 2 million won but Low in the group purchased product more than 7 million won.

성인골프 참가자의 라이프스타일과 소비자만족의 관계

임준택,최상범 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

The purpose of this study is to examine into the relationship between the lifestyle and the consumer satisfaction for adult participants in golf. As for the targets of this study, it used totally 342 adults (from more than 20 years old to less than 65 years old) with 221 men and 121 women, who are visiting the indoor golf clubs and the golf courses in Jeju area, for the actual analysis. As for the measurement tool used in this study, it quoted the questionnaire, which was used in "A Study on a Marketing Strategy Depending on Consumers' Buying-Behavior Styles for Sports Facilities" by Park Min-taek (1995), as a test of lifestyle. In terms of the statistical analysis used for data analysis, it conducted the reliability analysis, the frequency analysis, and the multiple regression, depending on a purpose of analysis. The conclusions, which were obtained according to these methods and procedures, are as follows. First, given seeing the differences between the socio-demographic factors and the lifestyles, it was shown that men are higher than women as for a sports-preference style, and as for a self-control style, the group in their less than 30s is highest and the next was higher in the group in theft more than 50s. Also, as for an influence style, it is highest in the group from their more than 30s to their less than 50s, and it was shown to be in order of the groups in their less than 30s and in their more than 50s. Depending on an income, it was shown that the less an income, the higher the styles of the sports preference and of the fashion pursuit. Even as for self-control, it was shown that the group with small income is highest. Depending on a job, the sports preference was shown to be high in the groups engaging in students, self-employers, and teaching-staff and office job, and the fashion pursuit was shown to be high in the groups of students, self-employers and housewives, Second, as for the differences of consumer satisfaction according to socio-demographic factors, it was shown that the internal satisfaction is highest in the group in their 30s-50s and the internal satisfaction is higher in order of more than in their 50s and of less than in their 30s. Depending on an income, it was shown that the external satisfaction is highest in the group in their 30-50s and is higher in order of groups in their less than 50s and in their less than 30s, Depending on a job, it was shown that the external satisfaction is highest in students and is higher in order of job categories such as profession and management, housewives, self-employers, and teaching-staff and office job. The internal satisfaction was shown to be highest in other job category, and was shown to be relatively high in job categories of housewives and self-employers. As for the differences of consumer satisfaction depending on demographic factors, it was shown that the internal satisfaction is highest in the group in their 30-50s, and is higher in order of their more than 50s and their Less than 30s. Depending on an income, it was shown that the external satisfaction is highest in the group in their 30-50s and is higher in order of the groups in their less than 50s and in their less than 30s. Depending on a job, it was shown that the external satisfaction is highest in students and is higher in order of job categories such as profession and management, housewives, self-employers, and teaching-staff and office job. It was shown that the internal satisfaction is highest in other job category and is relatively high in the job categories of housewives and self-employers. Third, it was shown that the lifestyle of adult participants in golf has a partial effect on consumer satisfaction. In Other words, it means that the higher a fashion-pursuit style, the internal satisfaction is higher and that the higher the self-control and the fashion pursuit, the external satisfaction gets higher.

한국인에서의 CYP2E1 유전자형

권준택,김형기,손동렬,염윤기 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Cytochrome P4502E1 (CYP2E1) is inducible by ethyl alcohol and activates procarcinogenic N-nitrosodimethylamine (NDMA), benzene, urethane and other lower molecular weight compound. CYP2E1 is also involved in metabolism of certain drugs, for example, alcohol, acetaminophen, tamoxifen, theophylline, fluorinated anesthetics, and chlorozoxazone. CYP2E1 activity was shown to be polymorphically distributed in humans and has been suggested to play a role in hepatocellular carcinoma and alcoholic related disorders. Although genetic predisposition to alcoholism and alcoholic liver disease has been reported, genetic susceptibility to alcoholic pancreatitis is still a matter of debate. The aims of this study were to investigate the allelic frequency of CYP2E1 Rsa I polymorphism in Koreans. We investigated the frequency distribution of CYP2E1 Rsa I polymorphism in 212 unrelated healthy Koreans and 42 hepatocellular carcinoma patients. Detection of the CYP2E1 alleles was performed by polymerase chain reaction and restriction fragment length analysis. The genomic DNA was isolated from peripheral blood with conventional phenol: chloroform extraction method. The allelic frequencies of c1 and c2 in healthy volunteers were 0.85 and 0.15, respectively. The rare type (c2/c2) of CYP2E1 Rsa I polymorphism was 0.005 in healthy volunteers.

니코틴 대사와 CYP2A6 유전자 빈도에 관한 민족적 다형성

권준택 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

사람의 CYP2A6 유전자는 CYP2A6*1A, CYP2A6*1B, CYP2A6*2, CYP2A6*3, CYP2A6*4 및 CYP2A6*5 대립 유전자가 밝혀져 있으며, nicotine-C 산화반응을 촉매하고 있다. 앞선 보고에 의하면, 일본인에서 nicotine 대사의 개인간 차이는 CYP2A6 유전자 다형성 (genetic polymorphism)과 관련이 있음이 증명된 바 있다. 저자는 Nicotine대사와 CYP2A6 대립 유전자들의 빈도에서 인종간의 차이를 증명하기 위해 209예의 한국인에서 nicotine대사와 CYP2A6의 유전자형을 연구하였다. Nicotine 껌을 투여 후 2시간에 혈장 cotinine/nicotine 비를 계산하여 nicotine대사의 지표로 사용하였으며, CYP2A6의 유전자형은 PCR-RFLP방법으로 결정하였다. 실험 결과 CYP2A6*1A, CYP2A6*1B, CYP2A6*4 및 CYP2A6*5 대립 유전자의 빈도는 한국인 (각각 45.7%, 42.8%, 11.0% 및 0.5%)과 일본인간에는 인종간의 차이를 보였다. 그러나 CYP2A6*2와 CYP2A6*3 대립 유전자형에는 어떠한 차이도 관찰할 수 없었다. 4명의 CYP2A6*4 homozygotes에서는 cotinine형성이 완전히 결여되었으며 그 결과는 일본인에서 관찰된 자료와 일치하였다. CYP2A6*4 heterozygotes에서는 이것이 없는 사람들에 비해 낮은 대사율을 보이는 경향이 있었으며, CYP2A6*1B 대립유전자를 지닌 사람들은 cotinine 형성 능력이 훨씬 높은 경향을 나타내었다. 니코틴 대사의 개인별 차이는 CYP2A6의 유전자 다형과 밀접한 관련이 있음을 확인하였다. 한국인에서 대사율의 probit plot 결과는 0.6의 antimode치를 보여 일본인에서 얻은 결과와 동일하였다. CYP2A6*1B/CYP2A6*4를 가진 한 명의 한국인에서 새로운 CYP2A6 돌연변이가 있는 것으로 보였다. 한국인의 probit plot 결과는 일본인보다 높은 비를 보였으며, 각 유전자형 군에서 한국인 군은 일본인 군에 비하여 유의하게 높은 대사율을 보였다. Cotinine 형성에서 인종간의 차이는 유전학적 소인 외에도 환경인자 및 식이 등의 인자들이 관여할 것으로 보인다.

한국인에서의 CYP2C19 유전자형의 다형성

권준택,김형기,손동렬,염윤기 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

The S-mephenytoin 4'-hydroxylase (CYP2C19) metabolizes a number of clinically used drugs and shows a marked interethnic difference in the incidence of the poor metabolizer (PM). In the present study, we genotyped 215 healthy unrelated Koreans (64 females, aged 20-41 yr, residing in Chungcheong province) for functionally defective alleles, CYP2C19_(m1) and CYP2C19_(m2). Detection of the normal (CYP2C19_(wt)) and defective alleles was performed by polymerase chain reaction/restriction enzyme analysis. The genomic DNA was isolated from peripheral blood. The allelic frequencies of the wild-type (CYP2C19_(wt)) and CYP2C19_(m1) were 72.6% and 27.4%, and the wild-type (CYP2C19_(wt)) and CYP2C19_(m2) were 85.1% and 14.9%, respectively. For each CYP2C19_(m1) and CYP2C19_(m2), the observed numbers of three genotypes were similar to those calculated in accordance with the Hardy-Weinberg equation. The frequencies of homozygotes for CYP2C19_(m1), CYP2C19_(m2) and compound heterozygotes were 9.7%, 2.8% and 4.2%, respectively. The mutants of CYP2C19 were identified in 36 subjects (16.7%). These results suggest that frequency of mutants of CYP2C19 in Koreans resembled the Orientals rather than Caucasians.

Aceclofenac 경구제제의 생물학적 동등성 연구

권준택,김형기,손동렬,염윤기 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

The bioequivalence of generic aceclofenac(Anacle^(®), Korea Pharma Co.) tablet to the aceclofenac tablet(Arital^(®), DaeWoong Pharmaceutical Co.) as a reference drug. Single dose of 100 mg aceclofenac was administered to 20 healthy male volunteers in a balanced, randomized crossover design with a washout between the two study periods. Blood samples were collected up to 12 hours and plasma concentration of aceclofenac was measured by well validated reverse phase high-performance liquid chromatography. Pharmacokinetic parameters were analyzed by non-compartmental analysis and ANOVA test was used for the statistical analysis of parameters. No statistically significant formulation, period, or sequence effect was encountered. Ninety percent confidence intervals of log transformed C_(max) and AUC_(t) were comprised in the stipulated 0.80-1.25 range. These results suggest that test formulation of aceclofenac is bioequivalent with the reference, both formulations in 100 mg tablet.

주5일 근무제 직장인의 스포츠활동 참가와 주관적 행복감의 관계

임준택,옥해안 한국스포츠리서치 2003 한국 스포츠 리서치 Vol. No.

The Purpose of this study to investigate the relationship of sports activity participation to subjective euphoria of office workers. To achieve this purpose, the subjects were selected by cluster random sampling of adults, in Daejon, in 2002 at present. There were 273 male, 215 female used for the analysis. The total was 489. Data were collected by employing a questionnaire. The variables of this analysis were demographic characteristics, sports activity participation, subjective happiness(positive affect scale and negative affect scale). The questionnaire for Life satisfaction had been developed by Diener, Emmons, Lersen & Griffin(1992) and standardized in Korean language by Lee Jong kil(1992). The questionnaire for Positive and Negative Affect by Watson, Clark & Tellgen(1988) and standardized in Korean language by Lee Ju Sung(1991) The collected data analyzed statistic program SPSS Windows 10.0 Version. The statistical analysis are descriptive statistics analysis, one-way ANOVA, multiple regression analysis and analysis of covariance. From the analyses of the data, this study reached he following conclusions: First, subjective euphoria is partially different by the demographic characteristics. Second, subjective euphoria is partially different by sports activity participation among employees. Third, degree of sports activity participation is positively influence on subjective euphoria.

혈장 중 theophyline 분석법 확립 및 약동학적 연구

김형기,권준택,염윤기 순천향대학교 의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

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알레르기 환자에서 혈청 IgE와 특이항원 양성율의 일치도

남해선,권준택,박준수 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

To evaluate usefulness of serum IgE to determine specific allergens, authors conducted priest IgE and MAST-CLA allergen-specific IgE assays in 78 allergic patients diagnosed at Soonchunhyang University Chunan Hospital during October 1998 through July 2000. During the study period, inhalent and food Korean panels were applied in 57 (73.1%) and 21 outpatients (26.9%), respectively. Kappa index was calculated to evaluate the agreement of priest IgE with Total IgE and allergen-specific IgE by MAST-CLA. 1. The men (±SD) age was 13.2 (±14.1) year-old and male accounted for 62.8%. 2. The overall positive rate of priest IgE was 66.7% and mean (±SD) level of priest IgE was 271.2 (±265.6) IU/ml 3. Patients with more than one specific allergen and with elevated total IgE by MAST-CLA were 25 (32.1%) and 33 (42.3%), respectively. 4. Significant agreement between total IgE and allergen-specific IgE was shown, but strength of agreement was slight (Kappa = 0.186, P = 0.046), 5. While no significant agreement between priest IgE and total IgE was observer, the priest IgE agreed significantly with allergen-specific IgE (Kappa = 0.153, P=0.043) In conclusion, our data suggested that serum priest IgE is more likely associated with allergen-specific IgE than total IgE in MAST-CLA assay. Furthermore, it is recommended that Korean panel of MAST-CLA should contain more specific allergens.