Analysis and Identification of ADP-Ribosylated Proteins of Streptomyces coelicolor M145

András Penyige,Judit Keserű,Ferenc Fazakas,Iván Schmelczer,Krisztina Szirák,György Barabás,Sándor Biró 한국미생물학회 2009 The journal of microbiology Vol.47 No.5

Mono-ADP-ribosylation is the enzymatic transfer of ADP-ribose from NAD+ to acceptor proteins catalyzed by ADP-ribosyltransferases. Using m-aminophenylboronate affinity chromatography, 2D-gel electrophoresis, in-gel digestion and MALDI-TOF analysis we have identified eight in vitro ADP-ribosylated proteins in Streptomyces coelicolor, which can be classified into three categories: (i) secreted proteins; (ii) metabolic enzymes using NAD+/NADH or NADP+/NADPH as coenzymes; and (iii) other proteins. The secreted proteins could be classified into two functional categories: SCO2008 and SCO5477 encode members of the family of periplasmic extracellular solute-binding proteins, and SCO6108 and SCO1968 are secreted hydrolases. Dehydrogenases are encoded by SCO4824 and SCO4771. The other targets are GlnA (glutamine synthetase I., SCO2198) and SpaA (starvation-sensing protein encoded by SCO7629). SCO2008 protein and GlnA had been identified as ADP-ribosylated proteins in previous studies. With these results we provided experimental support for a previous suggestion that ADP-ribosylation may regulate membrane transport and localization of periplasmic proteins. Since ADP-ribosylation results in inactivation of the target protein, ADP-ribosylation of dehydrogenases might modulate crucial primary metabolic pathways in Streptomyces. Several of the proteins identified here could provide a strong connection between protein ADP-ribosylation and the regulation of morphological differentiation in S. coelicolor.

Does Substantive ESG Lead to Higher Market Value in the Automobile Industry?

Andrés Guiral,문두철 한국모빌리티학회 2021 모빌리티연구 Vol.1 No.1

Previous research has found mixed results on the effect of ESG initiatives on market value. However, recent studies suggest that it is key to differentiate between two types of ESG initiatives: substantive and symbolic. Substantive ESG are costly and long-term oriented initiatives fully integrated into core business processes leading to critical intangible that creates shareholder value. On the contrary, symbolic ESG initiatives are more likely to serve as expenditures. In this paper, we explore whether substantive ESG initiatives positively affect the market value of automobile companies. This research question is less straightforward in the automobile industry than in other industries since substantive ESG has to compete with different core competencies in allocating resources, particularly intense R&D and marketing expenses. Additionally, given that substantive ESG is costly and requires a long-term horizon, we explore a potential U-shaped effect of substantive ESG investing, i.e., whether substantive ESG investing might financially pay off only for high levels but not for the case of low and moderate levels. From a large sample of automobile companies and using structural equation analysis (Partial Least Squares), we find that substantive ESG investing does lead to higher firm value. Importantly, our findings suggest that substantive ESG engagement does not compromise the positive impact of other critical intangibles, such as innovation and advertising intensity, on market value. Our findings also suggest that the effect of substantive ESG on market value is linearly positive rather than curvilinear. Our study has practical implications for both managers and investors.

Evaluation of the Mechanical and Fire Resistance Properties ofRigid Tannin Polyurethane Foams with Copper Oxide Nanoparticles

Andrés Díaz-Gomez,Mytzy Godoy,María Elizabeth Berrio,Jesús Ramirez,Andrés Felipe Jaramillo,Carlos Medina,Mary Montaño,Manuel Francisco Meléndrez 한국섬유공학회 2022 Fibers and polymers Vol.23 No.7

In this study, a polymerized polyurethane foam was formulated from tannins extracted from Pinus radiata aspolyol, and copper oxide nanoparticles (CuO-NPs) at a concentration of 2 % w/w as a nucleating agent. This produced areinforced foam with better mechanical properties and fire resistance. The impact of the addition of CuO-NPs on themechanical and thermal properties of the tannin foam was also studied. The properties studied were foaming process, foamdensity, cell morphology, flammability and thermal conductivity. The results revealed that the CuO-NPs incorporated in thefoam formulation act as nucleation centers, which decrease the pore size, strengthen the cell walls, and improve mechanicalproperties. The tannin samples showed good thermal resistance performance with a limit oxygen index (LOI) higher than21 %. The results obtained were contrasted with two foams, a commercial foam (SoudaFoam) and a foam generated with thesame dissociate, but with a commercial polyol (LHT-112). The foam with NPs had an elastic modulus of 3.7 MPa and stress(max) of 1.13 MPa, which was higher than those of the foams used as controls.

Relationship between Body Mass Index and Complications during the First 45 Days after Primary Total Hip and Knee Replacement: A Single-Center Study from South America

Andrés Correa-Valderrama,Willy Stangl-Herrera,Andrés Echeverry-Vélez,Erika Cantor,Tamy Ron-Translateur,Julio Cesar Palacio-Villegas 대한정형외과학회 2019 Clinics in Orthopedic Surgery Vol.11 No.2

Background: The goal of this study was to evaluate the relationship between body mass index (BMI) and early complications of total hip replacement (THR) and total knee replacement (TKR). Methods: This is a retrospective study of patients who underwent primary THR and TKR between January 2011 and December 2015. We included patients between 18 and 90 years of age with BMI less than 40 kg/m2 with a minimal postoperative follow-up time of 45 days. The primary outcomes were the presence of infection, mechanical complications (dislocation, fractures, arthrofibrosis, or neuropraxia), and thromboembolic events. Overweight and obesity were defined as a BMI of 25–29.9 kg/m2 and ≥ 30 kg/m2, respectively. Results: In total 750 patients were included (THR, 268; TKR, 482) with a mean age of 65.0 ± 12.4 years. The percentage of patients with normal weight, overweight, and obesity was 24.9% (n = 187), 41.7% (n = 313), and 33.4% (n = 250), respectively. The early complication rate was 8.9% (95% confidence interval [CI], 7.1% to 11.2%). Infection and mechanical complications were most prevalent. There was no statistically significant relationship between the incidence of complications and BMI (obesity vs. normal weight: hazard ratio [HR], 1.49; 95% CI, 0.72 to 3.06; p = 0.282); however, there was a tendency toward a greater risk of infectious complications in the patients with obesity (HR, 6.08; 95% CI, 0.75 to 49.16; p = 0.090). Patients with diabetes mellitus (DM) had more risk of infectious complications than those without DM (HR, 2.60; 95% CI, 1.00 to 6.79). Conclusions: There was no statistical relationship between BMI and early complications of THR and TKR. Nonetheless, there was a tendency toward a higher risk of infection in patients with some degree of obesity.

2D evaluation of crack openings using smeared and embedded crack models

André Luís Gamino,Osvaldo Luís Manzoli,José Luiz Antunes de Oliveira e Sousa,Túlio Nogueira Bittencourt 사단법인 한국계산역학회 2010 Computers and Concrete, An International Journal Vol.7 No.6

This work deals with the determination of crack openings in 2D reinforced concrete structures using the Finite Element Method with a smeared rotating crack model or an embedded crack model. In the smeared crack model, the strong discontinuity associated with the crack is spread throughout the finite element. As is well known, the continuity of the displacement field assumed for these models is incompatible with the actual discontinuity. However, this type of model has been used extensively due to the relative computational simplicity it provides by treating cracks in a continuum framework, as well as the reportedly good predictions of reinforced concrete members’ structural behavior. On the other hand, by enriching the displacement field within each finite element crossed by the crack path, the embedded crack model is able to describe the effects of actual discontinuities (cracks). This paper presents a comparative study of the abilities of these 2D models in predicting the mechanical behavior of reinforced concrete structures. Structural responses are compared with experimental results from the literature, including crack patterns, crack openings and rebar stresses predicted by both models.

Role of Culture in the Relations Between Hungary and Korea

András Szüts,István Medvigy 한국외국어대학교 EU연구소 2023 EU연구 Vol.0 No.65

Globalization, Instability and the New World Economic Order: comparing development strategies of Brazil and the Republic Korea

André Moreira Cunha,Andrés Ferrari 한국 포르투갈-브라질 학회 2009 포르투갈-브라질 연구 Vol.6 No.2

Este trabalho procura comparar as estratégias recentes de desenvolvimento de Brasil e Coréia do Sul, considerando suas experiências no período que se seguiu ao término da Segunda Grande Guerra Mundial, quando ambos se tornaram nações industrializadas. Procura‐se enfatizar os últimos quinze anos, que têm sido caracterizados, dentre outras coisas: (i) a conformação de uma nova ordem internacional, econômica e política, onde o unilateralismo do imediato pós‐guerra fria se transformou em um ambiente muito mais complexo; (ii) a emergência de novas potências, especialmente a China; (iii) uma crescente instabilidade financeira; e (iv) a decadência das estratégias nacionais inspiradas no Consenso de Washington.

Finite element computational modeling of externally bonded CFRP composites flexural behavior in RC beams

André Luís Gamino,Túlio Nogueira Bittencourt,José Luiz Antunes de Oliveira e Sousa 사단법인 한국계산역학회 2009 Computers and Concrete, An International Journal Vol.6 No.3

This paper focuses on the flexural behavior of RC beams externally strengthened with Carbon Fiber Reinforced Polymers (CFRP) fabric. A non-linear finite element (FE) analysis strategy is proposed to support the beam flexural behavior experimental analysis. A development system(QUEBRA2D/FEMOOP programs) has been used to accomplish the numerical simulation. Appropriate constitutive models for concrete, rebars, CFRP and bond-slip interfaces have been implemented and adjusted to represent the composite system behavior. Interface and truss finite elements have been implemented (discrete and embedded approaches) for the numerical representation of rebars, interfaces and composites.

Systems biology approaches to investigating the roles of extracellular vesicles in human diseases

András Gézsi,Árpád Kovács,Tamás Visnovitz,Edit I. Buzás 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

substantial attention as carriers of complex intercellular information. They have been implicated in a wide variety of biological processes in health and disease. They are also considered to hold promise for future diagnostics and therapy. EVs are characterized by a previously underappreciated heterogeneity. The heterogeneity and molecular complexity of EVs necessitates high-throughput analytical platforms for detailed analysis. Recently, mass spectrometry, nextgeneration sequencing and bioinformatics tools have enabled detailed proteomic, transcriptomic, glycomic, lipidomic, metabolomic, and genomic analyses of EVs. Here, we provide an overview of systems biology experiments performed in the field of EVs. Furthermore, we provide examples of how in silico systems biology approaches can be used to identify correlations between genes involved in EV biogenesis and human diseases. Using a knowledge fusion system, we investigated whether certain groups of proteins implicated in the biogenesis/release of EVs were associated with diseases and phenotypes. Furthermore, we investigated whether these proteins were enriched in publicly available transcriptomic datasets using gene set enrichment analysis methods. We found associations between key EV biogenesis proteins and numerous diseases, which further emphasizes the key role of EVs in human health and disease.

Lateral pterygoid muscle volume and migraine in patients with temporomandibular disorders

Sérgio Lúcio Pereira de Castro Lopes,André Luiz Ferreira Costa,Thiago de Oliveira Gamba,Isadora Luana Flores,Adriana Dibo Cruz,Li Li Min 대한영상치의학회 2015 Imaging Science in Dentistry Vol.45 No.1

Purpose: Lateral pterygoid muscle (LPM) plays an important role in jaw movement and has been implicated in Temporomandibular disorders (TMDs). Migraine has been described as a common symptom in patients with TMDs and may be related to muscle hyperactivity. This study aimed to compare LPM volume in individuals with and without migraine, using segmentation of the LPM in magnetic resonance (MR) imaging of the TMJ. Materials and Methods: Twenty patients with migraine and 20 volunteers without migraine underwent a clinical examination of the TMJ, according to the Research Diagnostic Criteria for TMDs. MR imaging was performed and the LPM was segmented using the ITK-SNAP 1.4.1 software, which calculates the volume of each segmented structure in voxels per cubic millimeter. The chi-squared test and the Fisher’s exact test were used to relate the TMD variables obtained from the MR images and clinical examinations to the presence of migraine. Logistic binary regression was used to determine the importance of each factor for predicting the presence of a migraine headache. Results: Patients with TMDs and migraine tended to have hypertrophy of the LPM (58.7%). In addition, abnormal mandibular movements (61.2%) and disc displacement (70.0%) were found to be the most common signs in patients with TMDs and migraine. Conclusion: In patients with TMDs and simultaneous migraine, the LPM tends to be hypertrophic. LPM segmentation on MR imaging may be an alternative method to study this muscle in such patients because the hypertrophic LPM is not always palpable.