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Alfia Dewi FADWATI,Fanny Hidayati,Mohammad Naiem 한국목재공학회 2023 목재공학 Vol.51 No.3
Eucalyptus pellita is one of the fast-growing tree species and has become predominant in Indonesian forest plantations. Meanwhile, tree breeding programs with clone development are the best way to provide greater genetic advantages. A better understanding of genetic control on growth and basic density in E. pellita is important for increasing wood productivity and quality. In this study, growth characteristics (tree height, diameter, and volume), basic density and its genetic parameters (heritability, genetic gain and genetic correlation) were determined. The number of clones tested in both trials was 50, divided into 5 blocks, and 5 trees/plot. The results showed that there were significant differences in growth and basic density among clones. There was an interaction between genetics and the environment further indicating the existence of unstable clones. The high heritability was found in tree height (0.82–0.86), diameter (0.82–0.90), and basic density (0.91–0.93). This implies that E. pellita has good opportunities for genetic improvement to increase wood productivity and quality. In addition, the results of genetic correlations among growth characteristics (height, diameter, and volume) and basic density showed positive moderate to highly significant value. It is suggested that these characters may be used to the advantage of the breeder for bringing improvement in these traits simultaneously. Therefore, this study provides important information of the genetic improvement of wood quality in E. pellita in Indonesia.
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El-Rahman, Atef Abd El-Mohsen Abd,El-Shafei, Sally Mohamed Abd El-Aziz,Ivanova, Elena Vladimirovna,Fattakhova, Alfia Nurlimanovna,Pankova, Anna Victorovna,El-Shafei, Mohamed Abd El-Aziz,El-Morsi, El-M Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17
Trichoderma spp. are known as a rich source of secondary metabolites with biological activity belonging to a variety of classes of chemical compounds. These fungi also are well known for their ability to produce a wide range of antibiotic substances and to parasitize other fungi. In search for new substances, which might act as anticancer agents, the overall objective of this study was to investigate the cytotoxic effects of Trichoderma harzianum and Trichoderma asperellum cultural filtrates against human cervical and breast cancer cell lines (HeLa and MCF-7 cells respectively). To achieve this objective, cells were exposed to 20, 40, 60, 80 and 100 mg/ml of both T. harzianum cultural filtrate (ThCF) and T. asperellum cultural filtrate (TaCF) for 24h, then the cell viability and the cytotoxic responses were assessed by using trypan blue and 3-(4,5-dimethylthiazol-2yl)-2,5-biphenyl tetrazolium bromide (MTT) assays. Morphological changes in cells were investigated by phase contrast inverted microscopy. The results showed that ThCF and TaCF significantly reduce the cell viability, have cytotoxic effects and alter the cellular morphology of HeLa and MCF-7 cells in a concentration dependent manner. A concentration of 80 and 100mg/ml of ThCF resulted in a sharp decline in the cell viability percent of HeLa and MCF-7 respectively (25.2%, 26.5%) which was recorded by trypan blue assay. The half-maximal inhibitory concentrations ($IC_{50}$) of ThCF and TaCF in HeLa and MCF-7 were recorded as 16.6, 12.0, 19.6 and 0.70mg/ml respectively by MTT assay. These results revealed that ThCF and TaCF have a substantial ability to reduce the viability and proliferation of human cervical and breast cancer cells.
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