http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 정봉남(Bong-Nam Joung) (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
-
-
감자 "추백"에 발생한 Tobacco mosaic virus의 특성
김정수 ( Joung Soo Kim ),김재현 ( Jae Hyun Kim ),최국선 ( Gug Seoun Choi ),채수영 ( Soo Young Choi ),김현란 ( Hyun Ran Kim ),정봉남 ( Bong Nam Joung ),최용문 ( Yong Mun Choi ) 한국식물병리학회 2003 식물병연구 Vol.9 No.2
An isolate of Tobacco mosaic virus (TMV) was isolated from potato cultivar ``Chubak`` showing vein clearing and mild mosaic at Namhae in Korea. This isolate, TMV-St, was differentiated from other tobamoviruses based on biological properties, serological relationships and nucleotide sequence analyses of coat protein genes. TMV-St caused typical symptoms on four indicator plants as compared to the tobamovirus of TMVU1, Pepper mild mottle virus (PMMoV), and Tomato mosaic virus (ToMV), which caused economic losses in Solanaceous vegetables, tomato, pepper, and eggplant. Remarkably, the TMV-St induced distinctly different symptom of systemic chlorotic spots on Chenophodium murale. On C. murale, Gomphorena globosa, and Nicotiana rustica, the four viruses were classed by the virulence of systemic or local infections. In serological test TMV-St antiserum showed a precipitation line with each other tabamovirus. The CP gene of TMV-St contain 477 nucleotides, and the nucleotides sequence was the most similar to that of TMV-U1.
-
TSWV 저항성 파프리카 품종 개발을 위한 분자 표지의 검정
김현정(Hyoun-Joung Kim),양희범(Hee-Bum Yang),정봉남(Bong Nam Chung),강병철(Byoung-Cheorl Kang) 한국원예학회 2008 원예과학기술지 Vol.26 No.4
Tomato spotted wilt virus(TSWV)는 최근에 국내 고추에서 발병하여 보고된 후, 점차적으로 발생피해가 증가하고 있는 병원성이 강한 바이러스이다. 국내 고추에는 저항성 품종이 알려진 바가 없어 TSWV 저항성 품종을 육성하는 일이 주목 받고 있으나, 저항성 고추를 육종하는 과정에서 저항성과 이병성을 선별하는 TSWV 접종도 쉽지 않은 것으로 보고되고 있다. 따라서, 효과적인 저항성 개체 선발을 위한 TSWV 분자 표지의 개발이 절실하다. 지금까지 개발된 TSWV 분자 표지로는 random amplified polymorphic DNA(RAPD) 5개와 cleaved amplified polymorphic sequence(CAPS)한 개가 보고되었으나, 이들 분자 표지를 저항성 개체 선발에 활용하기 위해서는 육종집단에 적용하여 재현성과 선별 정도를 조사하는 연구가 선행되어야 한다. 따라서 본 연구는 보고된 TSWV 분자 표지와 저항성 유전자(Tsw) 근처의 conserved ortholog set II(COSII), simple sequence repeat (SSR) 분자 표지를 수집하여 TSWV 저항성 재료인 PI152225, PI159236 계통, 저항성과 이병성 파프리카 품종을 이용하여 다형성을 조사하였다. RAPD, COSII, SSR 분자 표지는 다형성 밴드가 나타나지 않았으나 CAPS 표지인 SCAC568는 Zeraim 회사의 저항성 품종과 PI152225에서 이병성과 구별되는 다형성 밴드가 증폭되었고, BCF1 집단 92주 중 90주에서 표현형과 유전형이 일치하는 결과를 보였다. SCAC568 표지는 Zeraim 품종을 재료로 하는 TSWV 저항성 파프리카 품종(Marker-assisted selection) 육종에 유용하게 활용될 것으로 판단된다. The incidence of Tomato spotted wilt virus (TSWV) in pepper was recently reported and TSWV has been rapidly spreading in Korea. However, TSWV-resistant pepper cultivars have not been developed in Korea so far. To develop resistant cultivars, establishment of a reliable resistance screening method is most critical. Mechanical inoculation of TSWV is known to be very inefficient due to many escapes. Hence, deployment of molecular markers linked to the TSWV resistance gene (Tsw) will greatly improve efficiency of TSWV- resistant cultivar breeding. In order to deploy TSWV resistance-linked markers in a paprika breeding program, we applied previously developed molecular markers, which include five random amplified polymorphic DNA (RAPD) and one cleaved amplified polymorphic sequence (CAPS) markers, on six resistant and 11 susceptible paprika cultivars along with original TSWV-resistant materials, Capsicum chinense PI152225 and PI159236. In addition, several other markers close to the resistance locus (Tsw) were selected by a map comparison and applied on the same materials. Except for the CAPS marker SCAC568, no markers showed consistent polymorphisms between resistant and susceptible pepper materials. SCAC568 produced polymorphisms between three resistant cultivars of Zeraim and all susceptible cultivars. When the SCAC568 marker was applied on a BCF₁ population of 92 individuals, genotypes of SCAC568 were cosegregated with phenotypes in 90 individuals out of 92. These results demonstrate that SCAC568 can be deployed in pepper breeding programs in combination with TSWV-resistant cultivars from Zeraim.
-
Saccharomyces cerevisiae에서 GAL 또는 GAP 프로모터 조절에 의한 재조합 Inulinase의 발현 및 분비
남수완,임현정,정봉현,장용근 동의대학교 기초과학연구소 1997 基礎科學硏究論文集 Vol.7 No.1
To investigate the promoter effect on heterologous gene expression in S. cerevisiae, the recombinant plasmids pYI11, pYI12, pYI10-2, and pYIGP was constructed to contain the inulinase gene (INU1) as a reporter under the control of GAL10, GAL7, GAL1 and GAP promoters, respectively. When the yeasts transformants were cultivated on galactose-containing rich media, the cell growth reached to 36-39 OD_600 at 72 hours of cultivation. The specific growth rates of the cells harboring the four different plasmids decreased similarily : they dropped from 0.24 h^-1 during the glucose-consuming period to 0.04-0.10 h^-1 during the galactose-consuming period (gene expression phase for GAL promoter system). After the depletion of glucose, the expression of inulinase gene was started and reached to maximal levels of 4.3(GAL1 promoter), 4.0(GAL10 promoter), 3.8(GAL7 promoter), and 1.6(GAP promoter) unit/mL at 72 hours of cultivation. Based on the maximal expression level and activity staining on the plate, the promoter strength was in the order of GAL1, GAL10, GAL7, and GAP promoter. While the GAL-promoter systems showed a high plasmid stabilities of more than 78%, the GAP-promoter plasmid revealed a lower plasmid stability of 55%. Most of inulinase activity (98%) was found in the extracellular medium, indication that the secretion efficiency of inulinase is independent on the type of promoter.
ScienceON연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
